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EC number: 202-000-8 | CAS number: 90-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- Zahn-Wellens-Test
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-08-02 - 1989-08-30
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- no GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 Part 25 (Zahn Wellens Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- GLP compliance:
- no
- Remarks:
- study conducted before 2008
Test material
- Reference substance name:
- 6-amino-4-hydroxynaphthalene-2-sulphonic acid
- EC Number:
- 202-000-8
- EC Name:
- 6-amino-4-hydroxynaphthalene-2-sulphonic acid
- Cas Number:
- 90-51-7
- Molecular formula:
- C10H9NO4S
- IUPAC Name:
- 6-amino-4-hydroxynaphthalene-2-sulphonic acid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly industrial (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): aeration tank of a sewage treatment plant (Leverkusen-Bürrig, Germany), (predominantly industrial sewage with minor amounts of domestic effluents)
- Preparation of microbial inoculum for exposure: separation of the sludge by centrifugation (10 min at 3000 rpm), drying for 2 hours at 120°C
- Concentration of sludge: 1 g dry weight / L
- Sludge amount: 83.5 mL/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 208.4 mg/L
- Based on:
- test mat.
- Initial conc.:
- 480 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test apparatus: Zahn-Wells-equipment (3 - 4 L glass flask, stirrer, aeration)
- Test temperature: 20 ± 3 °C
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 9
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 96% degradation after 28 days has been determined with the reference substance aniline.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The study was conducted according to DIN 38412 Part 25 (Zahn Wellens Test) and OECD 302 B on the registered substance itself. The method is to be considered scientifically reasonable and suitable for the test item with some deficiencies in documentation, but the available information allows the conclusion that the test was properly conducted. Hence, the results can be considered as reliable to assess inherent biodegradability of the test item. A degradation of 9 % was determined, hence, the test item needs to be classified as not inherently biodegradable.
- Executive summary:
This report is based on a study report “Biodegradability of Gammasäure” (1989), the corresponding raw data and additional information from the executing laboratory “Institut für Umweltanalyse”, Dr. Kanne. Recent regulatory requirements necessitate this re-evaluation.
The study was conducted in accordance with DIN 38412 Part 25 (Zahn Wellens Test). This test method is in all essential parts identical with OECD Guideline 302 B.
A solution of Gammasäure in a mineral medium was inoculated with activated sludge and incubated under aerobic conditions. Degradation was followed by DOC determinations at 3h, 1d, 7 d, 14 d, 21 d, 27 d and 28 d. The reference compound Aniline showed 100 % degradation after 14 days.
Gammasäure showed:
1 % degradation after 3 hours
6 % degradation after 1 days
7 % degradation after 7 days
6 % degradation after 14 days
11 % degradation after 21 days
11 % degradation after 27 days
9 % degradation after 28 days
The result after 3 hours shows that adsorption does not play a significant role. Therefore the results describe biodegradation rather than elimination; the test item is considered to be “not inherently biodegradable“.
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