Pyrazolo[5,1-b]quinazolin-9(1H)-one, 2-methyl-

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-methyl-1H,9H-pyrazolo[3,2-b]quinazolin-9-one

EC Number:

616-079-5

Cas Number:

74336-55-3

Molecular formula:

C11 H9 N3 O

IUPAC Name:

2-methyl-1H,9H-pyrazolo[3,2-b]quinazolin-9-one

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, GER
- Age at study initiation: Young adult animals of a comparable weight were used.
- Weight at study initiation: 170g average weight
- Fasting period before study: The animale are given no feed 16 hours before administration, but water je available ad libitum.
- Housing: Five animals per cage (type: stainless steel wire mesh cages, DK-III).
- Diet: Ssniff R; SSNIFF Versuchstierdiäten, D-4470 Soest, FRG (ad libitum)
- Water: tap water ad libitum
- Acclimation period: at least one week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26°C
- Humidity (%): 45 - 75%
- Air changes (per hr): 15 -20 times/hour
- Photoperiod (hrs dark / hrs light): 12 h / 12 hr

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

0.5% aqueous carboxymethyl cellulose

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25% (w/v)
- Amount of vehicle (if gavage): 20 ml/kg
- Justification for choice of vehicle: The aqueous test substance preparation corresponds to the physiological medium.

Doses:

5000 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Recording of signs and symptoms < 15', 15', 30', 1 h, 2 h, 4 h and 5 h after administration of the test substance and then once each workday. Check for moribund and dead animals twice each workday and once on holidays. Body weights were recorded on days 0, 2, 7 and 13.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

None

Clinical signs:

other: No abnormalities detected

Gross pathology:

Organs: nothing abnormal detected

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met