2-chloro-4-nitrophenol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reveiwed journal

Justification for type of information:

Data is from peer reveiwed journal

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms.

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC Name: 2-chloro-4-nitrophenol
- Common Name: Nitrofungin
- Mol. formula: C6H4ClNO3
- Molecular Weight: 173.555 g/mol
- Smiles: c1(cc(c(O)cc1)Cl)[N+](=O)[O-]
- InChI: 1S/C6H4ClNO3/c7-5-3-4(8(10)11)1-2-6(5)9/h1-3,9H
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

- Method: The stock solutions of the compounds were prepared with distilled water and stored at 31C, and all the tested concentrations were nominal.

Test organisms (species):

other: Vibrio qinghaiensis nov.Q67

Details on inoculum:

- Laboratory culture: Department of Biology, East China Normal University.
- Initial biomass concentration: 5.87 × 10-3

Test type:

static

Water media type:

freshwater

Total exposure duration:

20 min

pH:

8.8-9

Nominal and measured concentrations:

1.018 mg/l Nominal concentration

Details on test conditions:

- No. of vessels per concentration (replicates): 9

Reference substance (positive control):

not specified

Key result

Duration:

20 min

Dose descriptor:

EC50

Effect conc.:

292.891 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Inhibition of bioluminescence

Remarks on result:

other: Bioluminescence of Q67 was then determined by Veritas TM luminometer. (-log EC50 = 4.40 mol/l)

Reported statistics and error estimates:

Concentration–response curves (CRCs) of all phenolic compounds and their mixtures were plotted on origin 7.5 software.

Validity criteria fulfilled:

not specified

Conclusions:

Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l.

Executive summary:

Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms Vibrio qinghaiensis nov.Q67. Test was performed in 96 well microplate. In the stud 36 blank controls (use deionized water in place of the toxicant) for a toxicant were performed. Vibrio was very sensitive to the chemical and thus exposed for 20 min only. Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l (-log EC50 = 4.40 mol/l).

Description of key information

Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms Vibrio qinghaiensis nov.Q67. Test was performed in 96 well microplate. In the stud 36 blank controls (use deionized water in place of the toxicant) for a toxicant were performed. Vibrio was very sensitive to the chemical and thus exposed for 20 min only. Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l (-log EC50 = 4.40 mol/l).

Key value for chemical safety assessment

EC50 for microorganisms:

292.891 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of 2-chloro-4-nitrophenol (619-08-9) on the microorganisms. The studies are as mentioned below:

In the first key study for 2-chloro-4-nitrophenol (619-08-9), Ecotoxicology and Environmental Safety 74 (2011), Determination of toxicity of chemical on Vibrio qinghaiensis sp.nov.Q67 by inhibiting the bioluminescence of microorganisms Vibrio qinghaiensis nov.Q67. Test was performed in 96 well microplate. In the stud 36 blank controls (use deionized water in place of the toxicant) for a toxicant were performed. Vibrio was very sensitive to the chemical and thus exposed for 20 min only. Based on the inhibition of Bioluminescence of Q67 due to the exposure of chemical 2-Chloro-4-nitrophenol for 20 min, the EC50 was 292.891 mg/l (-log EC50 = 4.40 mol/l).

 

Similarly in the second supporting study for 2-chloro-4-nitrophenol (619-08-9), (International Journal Environment Research 2011, and SAR QSAR Environ Res. 20110), toxicity of 2-chloro-4-nitrophenol was checked on the microorganisms. Determination of toxicity of chemical 2-Chloro-4-nitrophenol on the growth of Tetrahymena Pyriformis. Test performed in the static system. After the exposure of chemical growth inhibition was measured where 50% inhibition observed. Based on the growth inhibition of Tetrahymena Pyriformis due to the exposure of chemical, the IGC50 was 0.275 mg/l (– Log IGC50 = 1.59 mm/l).