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EC number: 229-539-1 | CAS number: 6598-63-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short term toxicity to aquatic invertebrate
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to invertebrate was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Intoxication value was estimated to be 1399.30 mg/l for Daphnia magna for 48 hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to aquatic invertebreate.
Toxicity to aquatic algae and cyanobacteria
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary
descriptor and considering the closest read across substances, the toxicity to algae was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Growth rate inhibition value was estimated to be 115.066 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to aquatic algae.
Additional information
Short term toxicity to aquatic invertebrate
Following studies include the experimental studies for the structurally similar read across to conclude the toxicity extent of trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) towards aquatic invertebrate is summarized as follows:
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to invertebrate was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Intoxication value was estimated to be 1399.30 mg/l for Daphnia magna for 48 hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to aquatic invertebreate.
The predicted data of target chemical is supported by the structurally similar read across Succinic acid (CAS: 110 -15 -6) from the publication Journal of the Water Pollution Control Federation 1980, suggests that The acute static tests were conducted as described in Ecological Research Series, EPA-600/3-75-009 ("Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians"). First instar Daphnia were used for all tests. The tests were conducted in duplicate for 48 hours at 22°C in a constant-temperature chamber. All test substance concentrations were nominal.
The Effective concentration to 50% of Daphnia magna is 374.2 mg/l at which 50% immobility was observed.
On the basis of the value obtained it can be concluded that the Succinic acid is not toxic to Daphnia magna at environmentally relevant concentrations and can be considered as “not Classified” under CLP regulation.
Further it is supported by another structurally similar read across Malonic acid(CAS: 141-82-2) from the similar publication Journal of the Water Pollution Control Federation 1980, also suggests thatIn Short term toxicity test, Daphnia magna was exposed to Malonic acid for 48 hrs.The effective concentration EC50 value for Daphnia magna when exposed to Malonic acid for 48 Hour is 275 mg/l.At which 50 % immobility was observed.On the basis of obtained value it can be concluded that the Malonic acid is not toxic to Daphnia magna and is considered to be “not classified” under CLP regulation.
Thus based on the effect concentrations which is in range of 275 mg/l to 1399.30 mg/L gives the conclusion that test substance trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.
Toxicity to aquatic algae and cyanobacteria
Following studies include the experimental studies for the structurally similar read across to conclude the toxicity extent of trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) towards aquatic algae is summarized as follows:
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary
descriptor and considering the closest read across substances, the toxicity to algae was predicted trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6). Growth rate inhibition value was estimated to be 115.066 mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to aquatic algae.
Similar prediction is done for the target chemical by using the three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the short term toxicity on green algae was predicted for test substancetrisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl}(CAS:6598 -63 -6). The average EC50 value was given by the third model i.e, Battery model. On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substancetrisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} is estimated to be 2809.387 mg/l for Pseudokirchneriella subcapitata during 72 hr exposure duration. Thus, on the basis of EC50 value,it can be concluded that the test chemicaltrisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl}can be considered asnon-toxicto green algae at environmentally relevant concentrations and can be considered "not-classified" as per the CLP classification criteria.
The Predicted data for the target chemical is assissted by the experimental study of structurally similar read acrossMetformin (CAS: 657 -24 -9) from the publication Toxicology Letters 2003,Toxicity to aquatic algae and cyanobacteria assessment was carried out to study the effects of Metformin on Desmodesmus subspicatus. Effective concentration to 50% of Desmodesmus subspicatus at which decrease in population growth rate was measured is 320 mg/L. It can be concluded that the Metformin is not toxic to the aquatic environment and can be considered as “not classified” as CLP regulation.
Similar experimental study of another structurally simialr read acrossBenzoic Acid (CAS: 65 -85 -0) from the publication Journal of Hazardous Materials 2009, suggests that theShort term toxicity of Benzoic acid toPseudokirchneriella subcapitata(green algae) study was carried out for 48 hrs.The study was based on the effects of the test compound onPseudokirchneriella subcapitatain a static fresh water system. On the basis of population of the test organismPseudokirchneriella subcapitata(green algae), the 48 hr EC50 value was determined to be 207.5 mg/l. Thus, based on this value, it can be concluded that the substance Benzoic acid can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.
Thus based on the effect concentrations which is in range of 115.066 mg/l to 2809.387 mg/L gives the conclusion that test substance trisodium 4-[(E)-2-{1-hydroxy-6-[({5-hydroxy-6-[(E)-2-phenyldiazen-1-yl]-7-sulfonatonaphthalen-2-yl} (CAS: 6598-63-6) was likely to be not toxic to algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.
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