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EC number: 224-030-0 | CAS number: 4170-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster)
- Principles of method if other than guideline:
- Dose administered by feeding
- GLP compliance:
- not specified
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- Crotonaldehyde
- EC Number:
- 224-030-0
- EC Name:
- Crotonaldehyde
- Cas Number:
- 4170-30-3
- Molecular formula:
- C4H6O
- IUPAC Name:
- but-2-enal
- Details on test material:
- molecular weight: 70.10
Source: Aldrich (JD 031397)
purity: 98-99%
Constituent 1
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: Canton-S
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- standard temperature: 23-25C
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- Solvent of choice for adult feeding study was solution of 5% sucrose in distilled water or 5% sucrose buffered to pH 6.8 with phosphate buffer
- Details on exposure:
- adult feeding : Males were treated for 3 days in glass shell vials containing a glass fiber disc soaked with 0.2 - 0.5 ml of solution
- Duration of treatment / exposure:
- feeding: 3 days
- Frequency of treatment:
- NA
- Post exposure period:
- for feeding: none
Doses / concentrations
- Remarks:
- Doses / Concentrations:
4000 ppm
Basis:
nominal conc.
- No. of animals per sex per dose:
- unknown
- Positive control(s):
- unknown
Examinations
- Tissues and cell types examined:
- not applicable
- Details of tissue and slide preparation:
- Not applicable
- Evaluation criteria:
- Lethal mutations were declared if no wild-type males were recovered among 20 or more Basc males or Bascl +/_ females. Retests were performed to confirm lethality. In these cases where a few F2 or F3 wild-type males survived among a large number of progeny, a lethal was declared if wild type males were less than 5% of the total Basc males or BACSL +/_ females.
A translocation was judged to have occured only in crosses with total counts of 20 or more progeny. Retests were performed to confirm translocations. T (Y;2;3) events were counted as two translocations. - Statistics:
- Lethal test: normal test [Margolin et al 1983]. Calculation of lethal frequencies and statistical tests were performed after clusters were removed.
Reciprocal translocation test: Conditional binomial test [Kastenbaum and bowman, 1970]
cluster analysis: by use of the formula for the cumulative Poisson distibution [Owens et al 1962] with a 0.01 alpha value. All data from a parental male producing a cluser were excluded.
Results and discussion
- Additional information on results:
- Negative for mutations if crotonaldehyde was administered via food
Any other information on results incl. tables
Results of Sex Linked Recessive Lethal Mutation Tests
Dose |
Route of Administration |
Percent Mortality |
Percent sterility |
Lethals |
Tests |
Total lethals |
Total tests |
Percent lethals |
||||
Br1 |
Br2 |
Br3 |
Br1 |
Br2 |
Br3 |
|||||||
4000 |
Feeding |
3 |
0 |
2 |
1 |
3 |
3150 |
2821 |
2606 |
6 |
8577 |
0.07 |
0 |
Feeding |
|
|
1 |
2 |
1 |
2399 |
2284 |
2107 |
4 |
6790 |
0.06 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative oral administration
Crotonaldehyde was negative in vivo for inducing mutation upon oral administration. - Executive summary:
Crotonaldehyde was tested for mutagenicity in Drosphilia melanogaster by adult feeding for the induction of sex-linked recessive lethal mutations in meotic and postmeitic germ cells stages on Canton-S males. Crotonaldehyde adminsitered via oral feed was not mutagenic in this assay.
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