Phosphoric acid, bis(phenylmethyl) ester, potassium salt (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

1996-01-21

Test material

Test material information

Constituent 1

Reference substance name:

potassium dibenzyl phosphate

EC Number:

616-628-9

Cas Number:

78543-37-0

Molecular formula:

C14-H14-O4-P.K

IUPAC Name:

potassium dibenzyl phosphate

Test material form:

solid

Details on test material:

Batch 97/8

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test material was prepared by a direct dispersion in water.
An amount of 100mg was dispersed in reconstituted water and the volume adjust to 1 litre to give the 100 mg/L test concentration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Justification for species other than prescribed by test guideline: common species
- Source: Daphnia magnawere maintained in a laboratory culture originating from a strain supplied by the Institut National de Recherche Chilique Appliquée (IRCHA), France.
- Feeding during test: No

ACCLIMATION
- No acclimatation: internal culture
- Type and amount of food: feed daily with a suspension of mixed algae (Predominantly Chlorella spp)

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Adult Daphnia were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water in a temperature controlled room at 21 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle. Each culture was fed daily with a suspension of mixed algae (predominantly Chlore/la spp.).
Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated 24 hours prior to the initiation of the test; the young daphnids produced overnight were then removed for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study.

Study design

Test type:

static

Water media type:

other: Reconstituted water

Total exposure duration:

48 h

Test conditions

Hardness:

approximately 270 mg/L CaCO3

Test temperature:

21 °C

pH:

7.8 to 7.9

Dissolved oxygen:

7.6 to 8.1 mg O2/L

Salinity:

Not applicable: Fresh water

Conductivity:

< 5 µS

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass jars
- Material, size, headspace, fill volume: 200 mL
- Aeration: Covered to prevent evaporation
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
Reconstituted water
- CaCl2.2H2O: 11.76 g/L
- MgSO4.7H2O: 4.93 g/L
- NaHCO3: 2.59 g/L
- HCl: 0.23 g/L

OTHER TEST CONDITIONS
- Adjustment of pH: if neccessary
- Photoperiod: 16h light and 8h darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility


RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation duriong the range finding test at 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Cumulative immobilization data in the range-finding study:

Nominal concentration (mg/L)	Cumulative immobilized Daphnia Initial Population: 10 per replicate
Control	0	0
0.10	0	0
1.0	0	0
10	0	0
100	0	0

 

Cumulative immobilization data in the definitive study:

Nominal concentration (mg/L)		Cumulative immobilized Daphnia Initial Population: 10 per replicate
		24 hours			48 hours
		Number per replicate	Total	%	Number per replicate	Total	%
Control	R1	0	0	0	0	0	0
	R2	0			0
100	R1	0	0	0	0	0	0
	R2	0			0
	R3	0			0
	R4	0			0

R1 – R4: Replicates 1 - 4

Physico-chemical measurments:

Nominal concentration (mg/L)		0 Hours			24 hours	48 hours
		pH	mg O2/L	T° (°C)	T° (°C)	pH	mg O2/L	T° (°C)
Control	R1	7.9	8.1	21	21	7.8	7.6	21
	R2	7.9	8.1	21	21	7.8	7.7	21
100	R1	7.9	8.1	21	21	7.8	7.7	21
	R2	7.9	8.1	21	21	7.8	7.7	21
	R3	7.9	8.1	21	21	7.9	7.7	21
	R4	7.9	8.1	21	21	7.8	7.6	21

R1 – R4: Replicates 1 - 4

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the Dibenzyl Potassium Phosphate on Daphnia Magna has been determinated to be greater than 100 mg/L

Executive summary:

The acute toxicty on aquatic invertebrates of Dibenzyl Potassium Phosphate has been determinated according to OECD 202 test guideline in compliance with GLP.

The 48h-EC50 was determinated to be greater than 100 mg/L.