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Diss Factsheets
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EC number: 228-782-0 | CAS number: 6358-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979-06-12 to 1979-07-03
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well conducted study prior to GLP and OECD-TG.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
- Principles of method if other than guideline:
- Administration of the test substance by oral gavage. 10 Animals per dosage group. After administration observation of lethal symptoms, mortality and time of death. Weekly weighting of animals. Necropsy and gross pathology of the dead animals and all surviving animals sacrificed after end of 14 day observation time.
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4-chloro-2,5-dimethoxyaniline
- EC Number:
- 228-782-0
- EC Name:
- 4-chloro-2,5-dimethoxyaniline
- Cas Number:
- 6358-64-1
- Molecular formula:
- C8H10ClNO2
- IUPAC Name:
- 4-chloro-2,5-dimethoxyaniline
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- - Strain: Hoe WISKf(SPF71)
- Source: Hoechst AG, own breed
- Weight: 180 - 196 g (mean: 187.2 g +/- 4.92 g)
- Diet: Altromin 1324, ad libitum
- Water: tap water, ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Details on oral exposure:
- - Concentration of test substance in vehicle 25%
- Fasting 16 hours before and 2 hrs after administration. - Doses:
- 800, 2000, 5000 mg/kg bw
- No. of animals per sex per dose:
- 10 females
- Control animals:
- no
- Details on study design:
- - Weekly weighing of animals during 14 day observation period after administration of the test substance
- Necropsy and gross pathology of the dead animals and all surviving animals sacrificed after end of 14 day observation time. - Statistics:
- - LD50 using probit analysis (Method of Linder and Weber)
- Confidence levels using method of Fieller
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 260 mg/kg bw
- 95% CL:
- 798 - 1 850
- Mortality:
- 800 mg/kg: 2 of 10 animals
2000 mg/kg: 8 of 10 animals
5000 mg/kg: 10 of 10 animals - Clinical signs:
- other: drowsiness, crouched position, ruffeled fur, uncoordinated walk, dark yellow brown discoloration of urine, abdominal position, lateral position, irregular breathing rate, increased lacrimal discharge, narcosis.
- Gross pathology:
- Necrosis of dead animals: hepatic lobules altered, bladder filled with brown fluid
Necrosis of surviving animals sacrificed at end of study: None.
Applicant's summary and conclusion
- Interpretation of results:
- other: weakly toxic
- Remarks:
- Criteria used for interpretation of results: other: Spector "Handbook of Toxicology"
- Conclusions:
- In the present study a LD50 of 1260mg/kg bw was determined.
- Executive summary:
In the present study a LD50 of 1260mg/kg bw was determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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