Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 412-540-8 | CAS number: 22471-55-2 ET 344 SP
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- The inoculum was prepared by mixing equal volumes of following
three inoculum samples.
1)Filtrate of standard activated sludge
Source ; Chemical Biotesting Center, Chemical Inspection and Testing
Institute, Japan
Date of receipt ; January 21, 1993
2)Effluent from domestic sewage treatment plant
Source ; Research Center, Mitsubishi Kasei Co.
Date of collection ; April 9, 1993
3)River surface water
Source ; Kamoshida River
Date of collection ; April 9, 1993 - Duration of test (contact time):
- 28 d
- Initial conc.:
- 0.92 other: mg
- Based on:
- test mat.
- Details on study design:
- Test bottle :Run #I ; aniline + inoculum + standard dilution water
Run #II ; inoculum + standard dilution water
Run #III ; test substance + inoculum + standard dilution water
Run #IV ; standard dilution water
Run #V ; test substance + water
EXPOSURE
The test was conducted according to the following conditions.
1)Apparatus
Incubator, TAITEC Co., Model LX-30002 )
Condition
Temperature :20 +/- 1°C
Exposure period :28 days (under dark condition)
Test volume :300 mL
Concentration :test substance (Run III and V) ;3.07 mg/L aniline* (Run #I) ;2.00 mg/Lcomposite inoculum (Run #1-III ) ; 100 uL/L(*: Reference substance ; Showa Chemicals, Lot No.SC-3131)
MEASUREMENT OF DISSOLVED OXYGEN CONCENTRATION
The concentration of the dissolved oxygen in the BOD bottle was measured at day 0, 14 and 28.
1)Apparatus : DO meter ; Central Kagaku Co., Model UD-1
DOC MEASUREMENT
The concentration of the DOC was measured as follows.
1)Apparatus : TOC analyzer, Shimadzu Co., model TOC-5000
2)Condition : Furnace temperature : 680°C (TC)
Air flow rate : 150 ml/min.
Sensitivity : X1
Injection volume : 40 ul
3)Calibration curve
The following standard solutions were injected into the TOC analyzer. The calibration curve was prepared by the data processor of the analyzer .
Standard solutions
TC(total carbon) ; 10 mg-C/1 aqueous solution of potassium hydrogen phthalate.
IC(inorganic carbon); 10 mg-C/1 aqueous solution of sodium hydrogencarbonate.
4)Measurement of DOC in the bottles
After measuring dissolved oxygen concentration, 10 ml of the test solution in each bottle was transferred into 10 ml centrifuge tube and centrifuged at 3000 rpm for 10 minutes.
Five ml of supernatant was used for measurement of DOC.
pH MEASUREMENT
After sampling the test solution for DOC measurement, pH of each residual test solution was measured at day 0, 14 and 28.
1)Apparatus : pH meter, Denki Kagaku Keiki, Model COM-10 - Reference substance:
- aniline
- Test performance:
- ACTIVITY OF INOCULUM
Degradability of aniline based on oxygen consumption was 66, 68% at day 28. The activity of the inoculum was judged as satisfactory. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 0
- Sampling time:
- 28 d
- Details on results:
- DEGRADABILITY BASED ON OXYGEN CONSUMPTION
Oxygen consumption* in Run #II was 0.0, -0.1 mg/l, and both 0.1 mg/l in Run #V at day 28. (*:Theoretical value = 8.17 mg)
Degradability based on oxygen consumption were calculated to be 0, -1% i n Run #II, and both 1% i n Run #V.
DEGRADABILITY BASED ON RESIDUAL TEST SUBSTANCE CONCENTRATION
The residual test substance* was detected at concentrations of 2.93 and 2.91 mg/l i n Run #X , and 3.05, 2.93 mg/l in Run #V.
Degradability based on the residual test substance concentration were calculated to be 3, 4% i n Run #X and 2, 5% i n Run #V, respectively.
(*:Theoretical value = 3.07 mg/l)
DEGRADABILITY BASED ON DOC
DOC* i n Run #III was 1.05, 1.03 (as corrected with the average value of Run #II), and 1.59, 1.45 mg/l in Run #V. (*:theoretical value = 2.23 mg/l)
Degradability was not calculated because the test substance was insoluble in water.
pH MEASUREMENT
pH of the test solutions at day 28 were both 7.1 i n Run #X, and were 5.9, 5.8 in Run #V. - Results with reference substance:
- Points of degradation plot based on oxygen consumption:
57 % degradation after 14 d
67 % degradation after 28 d - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Based on the results of oxygen consumption, it was concluded that the test substance was not readily biodegradable under the conditions of the Closed Bottle Test.
- Executive summary:
STUDY TITLE
Ready biodegradability test of ET-344-SP
METHOD
The test was conducted according to the method described as "Ready Biodegradability, Closed Bottle Test" in the OECD Guidelines for Testing of Chemicals No.301D.
Test bottle :
Run #I ; aniline + inoculum + standard dilution water
Run #II ; inoculum + standard dilution water
Run #III ; test substance + inoculum + standard dilution water
Run #IV ; standard dilution water
Run #V ; test substance + water
Measurement :
Dissolved oxygen concentration ; at day 0, 14, 28
Residual test substance concentration ; at day 0, 14, 28
Dissolved organic carbon concentration (DOC) ; at day 0, 14, 28
RESULTS
Degradability ( % )
Run# III-1 III-2 V-1 V-2 oxygen consumption 0 -1 1 1 test substance 3 4 2 5 DOC not calculated because test substance was insoluble in water. CONCLUSION
Based on the results of oxygen consumption, it was concluded that the test substance was not readily biodegradable under the conditions of the Closed Bottle Test.
Reference
Description of key information
Based on the results of oxygen consumption, it was concluded that the test substance was not readily biodegradable under the conditions of the Closed Bottle Test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.