Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-409-2 | CAS number: 3087-16-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source .
Data source
Reference
- Reference Type:
- secondary source
- Title:
- SIDS Initial Assessment Report For SIAM 13, CAS no: 16470-24-9(Fluorescent Brightener 220)
- Author:
- OECD SIDS SIAM 13
- Year:
- 2 001
- Bibliographic source:
- SIDS Initial Assessment Report for SIAM 13, Bern, Switzerland, 6-9 November 2001
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: Repeated insult patch test
- Principles of method if other than guideline:
- The skin sensitization study of Fluorescent Brightener 220 was performed by repeated insult patch test.
- GLP compliance:
- not specified
- Type of study:
- other: Repeated insult patch test
- Justification for non-LLNA method:
- No data available
Test material
- Reference substance name:
- Tetrasodium 4,4'-bis[[4-[bis(2-hydroxyethyl)amino]-6-(4-sulphonatoanilino)-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate]
- EC Number:
- 240-521-2
- EC Name:
- Tetrasodium 4,4'-bis[[4-[bis(2-hydroxyethyl)amino]-6-(4-sulphonatoanilino)-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonate]
- Cas Number:
- 16470-24-9
- Molecular formula:
- C40H44N12O16S4.4Na
- IUPAC Name:
- tetrasodium 2,2'-ethene-1,2-diylbis[5-({4-[bis(2-hydroxyethyl)amino]-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)benzenesulfonate]
- Test material form:
- solid
- Details on test material:
- Name of the test chemical:C.I. Fluorescent Brightener 220
Molecular Formula: C40H44N12O16S4.4Na
Molecular Weight:1165.048 g/mol
Substance Type: Organic
Physical State: Solid
Constituent 1
- Specific details on test material used for the study:
- Details on test material
Name of the test chemical:C.I. Fluorescent Brightener 220
Molecular Formula: C40H44N12O16S4.4Na
Molecular Weight:1165.048 g/mol
Substance Type: Organic
Physical State: Solid
In vivo test system
Test animals
- Species:
- other: human
- Strain:
- not specified
- Sex:
- female
- Details on test animals and environmental conditions:
- No data available
Study design: in vivo (non-LLNA)
Induction
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- 0.1%
- Day(s)/duration:
- 48hr
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- 0.1%
- Day(s)/duration:
- 48 hr
- No. of animals per dose:
- 103 female
- Details on study design:
- Details on study design
RANGE FINDING TESTS:
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures:10
- Exposure period:48hr per application
- Test groups:103
- Control group: No data available
- Site: back
- Frequency of applications: After 48hr for 10 application
- Duration:20 days
- Concentrations: 0.1%
B. CHALLENGE EXPOSURE
- No. of exposures: one
- Day(s) of challenge: After 14 days of last induction patch test
- Exposure period:48 hr
- Test groups:103
- Control group: No data available
- Site: back
- Concentrations: 0.1%
- Evaluation (hr after challenge):48hr
OTHER: No data available - Challenge controls:
- No data available
- Positive control substance(s):
- no
Study design: in vivo (LLNA)
- Statistics:
- No data available
Results and discussion
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 0.1%
- No. with + reactions:
- 0
- Total no. in group:
- 103
- Clinical observations:
- no indication of skin sensitization after the challenge
- Remarks on result:
- no indication of skin sensitisation
- Remarks:
- Not skin sensitizing in human
Applicant's summary and conclusion
- Interpretation of results:
- other: Not sensitizing
- Conclusions:
- The skin sensitization study of Fluorescent Brightener 220 (16470-24-9) was performed by repeated insult patch test in human was determined to be not skin sensitizing
- Executive summary:
The skin sensitization study of Fluorescent Brightener 220(16470-24-9) was performed by repeated insult patch test in 103 female volunteers using 0.1% aqueous solution.
In induction phase, 10 repeated patch applications was done using 0.1% test solution for 48hr per application at back of the volunteers.
In challenge phase, After 14 days rest period challenge application of 0.1% test solution was applied on back of the subject for 48hr.No skin sensitizing reaction observed after 48hr of challenge application .Hence it is considered thatFluorescent Brightener 220(16470-24-9) was not skin sensitizing in human
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.