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Diss Factsheets
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EC number: 944-232-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 17, 2015 - April 24, 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Deviations:
- no
- GLP compliance:
- no
- Type of method:
- flask method
- Key result
- Water solubility:
- < 1 other: mg (TOC) /L
- Conc. based on:
- not specified
- Temp.:
- 20 °C
- pH:
- 6
- Conclusions:
- The water solubility of the test item was determined at a temperature of 20 °C according to OECD 105. Due to its insolubility in aqueous and organic solvents, it is not possible to analytically quantify Vat Black 25. Hence, the total organic carbon content (TOC) was measured. It was determined to be: < 1 mg (TOC) / L.
- Executive summary:
Study performed to determine the water solubility of the test substance using the flask method.
Study performed in accordance with the following guidelines:
EU Method A.6 (Water Solubility)
OECD Guideline 105 (Water Solubility)
In a simple preliminary test, mixtures of water and test item are prepared and visual assessed. The approximate water solubility of the test item, resulting from the preliminary test, indicates which of the two methods for the determination of the water solubility is applicable. Whereas the column elution method covers solubilities below 10 mg/L the flask method is suitable for solubilities above 10 mg/L.
Additionally, the results of the preliminary test provide information of the appropriate amount of test item to be used in the main test.
Flask method
The quantity of test item necessary to saturate the desired volume of water is estimated from the preliminary test. About five times that quantity is weighed into each of three glass vessels fitted with glass stoppers. The batches are stirred for different times at 30 °C (24 h experiment, 48 h experiment, 72 h experiment) in tightly closed flasks. A blank sample consisting of water only is stirred for 72 h at 30°. After equilibration (further stirring for 24 h at 20 °C), the suspensions and the blank are taken and prepared for the analytical determination. The pH values of the saturated solutions are determined.
Results
Preliminary visual estimation of the water solubility
Different amounts of the test item were stirred 100 mL and 500 ml distilled water, respectively and the mixtures were visually checked for any undissolved parts.
The preliminary visual experiments showed that the solubility of the test item in water is below 5 mg/l(without correction for the purity). As the test item is insoluble in organic solvents it is impossible to coat the sea sand of the columns with the test item. For this reason the column elution method is not applicable and the water solubility had to be determined by the flask method.
Results of the flask method
The flask method was performed. After the equilibration time precipitate was observed in the flasks of the experiments. No particles were seen in the blank experiment. As the test substance was neither soluble in water nor in organic solvents, the concentration of the test item dissolved in water was determined by measurement of the dissolved TOC.
The TOC concentration in the aqueous samples was determined to be < 1 mg/l TOC.
The water solubility of the test item was determined at a temperature of 20 °C according to OECD 105. Due to its insolubility in aqueous and organic solvents, it is not possible to analytically quantify Vat Black 25. Hence, the total organic carbon content (TOC) was measured. It was determined to be: < 1 mg (TOC) / L.
Reference
Individual results
Preliminary visual estimation of the water solubility
Different amounts of the test item were stirred 100 mL and 500 ml distilled water, respectively and the mixtures were visually checked for any undissolved parts.Table 2 shows the results from the preliminary test.
Table 2: Appearance of the test mixtures
Amount of test item in mg |
Total volume in ml |
Appearance of mixture |
12.6 |
100 |
undissolved1) |
11.7 |
500 |
undissolved1) |
2.4 |
500 |
undissolved1) |
1): After adding the indicated amount of water, the solution was stirred for approx. 72 h.
The preliminary visual experiments showed that the solubility of the test item in water is below 5 mg/l(without correction for the purity). As the test item is insoluble in organic solvents it is impossible to coat the sea sand of the columns with the test item. For this reason the column elution method is not applicable and the water solubility had to be determined by the flask method.
Results of the flask method
The flask method was performed After the equilibration time precipitate was observed in the flasks of the experiments. No particles were seen in the blank experiment.As the test substance was neither soluble in water nor in organic solvents,the concentration of the test item dissolved in water was determined by measurement of the dissolved TOC.The water solubility was calculated from the measured and unrounded concentration values and not from the rounded values given inTable3.
Table3:Solubility of the test item in water – experiments A to D
Experiment |
24 h experiment |
48 h experiment |
72 h experiment |
Blank experiment (72 h) |
Amount of test item (mg) |
5.4 |
5.4 |
5.6 |
-- |
Amount of distilled water (ml) |
200 |
200 |
200 |
200 |
Stirring time at 30 °C (h) |
24 |
48 |
72 |
72 |
pH at 23°C |
5.9 |
6.1 |
6.2 |
|
Measured TOC concentration |
< 0.51 |
< 0.51 |
< 0.51 |
|
The TOC concentration in the aqueous samples was determined to be < 1 mg/l TOC.
Description of key information
Key value determined in a laboratory test using the flask method, performed in accordance with OECD Guideline 105 and EU Method A.6.
The water solubility of the test item was determined at a temperature of 20 °C according to OECD 105. Due to its insolubility in aqueous and organic solvents, it is not possible to analytically quantify Vat Black 25. Hence, the total organic carbon content (TOC) was measured. It was determined to be: < 1 mg (TOC) / L.
Key value for chemical safety assessment
- Water solubility:
- 1 mg/L
- at the temperature of:
- 20 °C
Additional information
The water solubility of the test item was determined at a temperature of 20 °C according to OECD 105. Due to its insolubility in aqueous and organic solvents, it is not possible to analytically quantify Vat Black 25. Hence, the total organic carbon content (TOC) was measured. It was determined to be: < 1 mg (TOC) / L.
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