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EC number: 700-208-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-09-23 - 2009-01-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to an internationally accepted guideline. All study parameters are based on the specific guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- A mobile phase consisting of 14 % methanol in water was used instead of 15 % methanol in water.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analysis of test solutions for P 70/05/S-4 0 hours and 48 hours after test start resulted in concentrations ranging between 94 and 107 % of nominal. The test concentrations were found within ± 20 % of nominal throughout the test. Therefore, all results are based on the nominal concentration.
- Vehicle:
- yes
- Details on test solutions:
- The test item was dissolved directly in dilution water.
The test solutions were prepared in the following way:
- Weighing of 50.0 mg P 70/05/S-4
- Filling with dilution water up to 250 mL
(= stock solution A, containing 200 mg P 70/05/S-4/L)
The test solutions of the chosen concentration was prepared directly in the test vessels by dilution of the stock solution with dilution water immediately before the transfer of the Daphnia into the test vessels. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Holding and breeding in the test facility under standardised laboratory conditions,
Test animals derived from a healthy stock, not first brood progeny,
Age of the Daphnia used in the test: < 24 hours Holding conditions,
Breeding medium: M 4 medium (according to ELENDT, OECD 2002)
Breeding conditions: breeding was performed in glass beakers (50 mL) under the same conditions as in the test; test animals were separated from the adults by means of a pipette
Food: Alga Desmodesmus subspicatus, origin: Albrecht-v.-Haller-lnstitut für Pflanzenwissenschaften, Nikolausberger Weg 18, D-37073 Göttingen
Feeding: daily; Daphnia of different age were fed with a defined suspension of algae:≤ 1 day old: 0.5 mL/50 mL M 4, 3 to 10 days old: 1.0 mL/50 mL M 4, > 10 days old: 1.5 mL/50 mL M 4 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2 mmol/L CaC03 which corresponds to a hardness of 200 mg/L CaC03 (valid: > 140 and < 250 mg/L).
- Test temperature:
- 19.5-20.1 °C
- pH:
- 7.92
- Dissolved oxygen:
- 8.58 mg/L
- Salinity:
- The sum of calcium and magnesium ions in the dilution water was 2.5 mmol/L. The proportion of Ca to Mg ions was 4:1 and of Na to K ions 10:1. The acid capacity Ka of the solution was 0.8 mmol/L.
- Nominal and measured concentrations:
- Analysis of test solutions for P 70/05/S-4 0 hours and 48 hours after test start resulted in concentrations ranging between 94 and 107 % of nominal. The test concentrations were found within ± 20 % of nominal throughout the test. Therefore, all results are based on the nominal concentration.
- Details on test conditions:
- Test vessel:glass beaker
Test volume:10 ml
Number of Daphnia/ test vessel (= replicate): 5
Number of test vessels/ concentration: 4 + 3 (1 for measuring, 1 for analysis, 1 retain specimen)
Number of daphnia/concentration: 20
Loading: 2 mL test solution per Daphnia
Test medium: dilution water: reconstituted water according to ISO 6341, prepared 1 day in advance, using deionised water conductivity of deionised water: ≤ 10 µ S/cm (measured 0.600 µS/cm) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The short-term toxicity of N-[3(5)-Methyl-1 H-pyrazol-1 -yl-methyl]acetamide to aquatic invertebrates was determined according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation). Under laboratory conditions no effects of the test item on immobilisation of daphnids were determined. The NOEC (no observed effect concentration) after 48 hours was determined to be 100 mg/L.
- Executive summary:
The short-term toxicity of N-[3(5)-Methyl-1 H-pyrazol-1 -yl-methyl]acetamide to aquatic invertebrates was determined according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation). Under laboratory conditions no effects of the test item on immobilisation of daphnids were determined. The NOEC (no observed effect concentration) after 48 hours was determined to be 100 mg/L.
Reference
Treatment group (mg P 70/05/S-4/L, nominal) |
Immobilised Daphnia (number) |
Immobility of Daphnia(%) |
||
|
24h |
48h |
24h |
48h |
Control (test medium) |
0 |
0 |
0 |
0 |
100.0 |
0 |
0 |
0 |
0 |
Description of key information
The short-term toxicity of N-[3(5)-Methyl-1 H-pyrazol-1 -yl-methyl]acetamide to aquatic invertebrates was determined according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation). Under laboratory conditions no effects of the test item on immobilisation of daphnids were determined. The NOEC (no observed effect concentration) after 48 hours was determined to be 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The NOEC (no observed effect concentration) after 48 hours was determined to be 100 mg/L.
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