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EC number: 916-737-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study done to guideline, in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane
- EC Number:
- 272-669-9
- EC Name:
- 2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane
- Cas Number:
- 68901-32-6
- Molecular formula:
- C15H24O2
- IUPAC Name:
- 2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane
- Test material form:
- other: liquid
- Details on test material:
- Please note that although the substance is stated as the mono-constituent substance 2-(6-methyl-8-(-1-methylethyl)bicyclo(2.2.2)oct-5-en-2(3)-yl-1,3-dioxolane in the report, analytical data on the same batch shows that the test substance is in fact the multi-constituent substance Reaction mass of 2-(7-isopropyl-5(6)-methylbicyclo[2.2.2]oct-5-en-2-yl)-1,3-dioxolane and 2-(8-isopropyl-5(6)-methylbicyclo[2.2.2]oct-5-en-2-yl)-1,3-dioxolane.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analytical confirmation of exposure concentrations were taken at the start, after 24 and 72 hours of exposure. Samples taken from all concentrations were analysed. The actual concentrations ranged from 0.90 to 9.5 mg/l, at the start of the test. Measured concentrations decreased to 63-74% of initial at the end of the test. Based on these results, the Time Weighted Average concentrations were calculated to correspond to 0.73, 1.3, 2.5, 4.1 and 7.5 mg/l in solutions containing 10, 18, 32, 56 and 100% WSF, respectively.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Nominal and measured concentrations:
- Nominal: 10, 18, 32, 56 and 100% of water soluble fraction
Measured: 0.73, 1.3, 2.5, 4.1 and 7.5 mg/L (time weighted average) - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 7.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL not determined
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 4.2-5.4
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: not applicable
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: not applicable
- Duration:
- 72
- Dose descriptor:
- EC10
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 3.1-3.8
- Duration:
- 72
- Dose descriptor:
- EC10
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.9-1.5
- Results with reference substance (positive control):
- The reference test was carried out to check the sensitivity of the test system used by WIL Research Europe to Potassium dichromate (Merck, Art. 1.04864, Batch K44879664). Closed test vessels were used as to mimic testing a volatile test article and results were compared with standard ‘open’ systems.
Algae were exposed for a period of 72 hours to K2Cr2O7 (Potassium dichromate) concentrations of 0.18, 0.32, 0.56, 1.0, 1.8 and 3.2 mg/l and to a control. The initial cell density was 1.0 x 104 cells/ml.
The EC50 for growth rate inhibition (72h-ERC50) was 1.5 mg/l with a 95% confidence interval ranging from 1.4 to 1.7 mg/l. The historical ranges for growth rate inhibition using standard ‘open’ systems range between 0.82 and 2.3 mg/l. Hence, the 72h-ERC50 for the present test in closed systems is within the historical range.
The EC50 for yield inhibition (72h-EYC50) was 0.41 mg/l with a 95% confidence interval ranging from 0.38 to 0.44 mg/l. The historical ranges for yield inhibition using standard ‘open’ systems lie between 0.43 and 1.1 mg/l. Hence, the 72h-EYC50 for the present test in closed systems was just below this range.
It was concluded that the sensitivity of algae exposed in closed test systems towards Potassium dichromate was not significantly different from the sensitivity of algae exposed in standard ‘open’ reference studies during the past years. These results indicate that the algae are responding normally to toxicant stress under the modified "sealed system" test conditions and that these deviations from test guidelines do not affect the results of this study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
Under the conditions of the present study with Pseudokirchneriella subcapitata, Glycolierral inhibited growth rate of this fresh water algae species significantly at a TWA concentration of 4.1 mg/l and higher.
The EC50 for growth rate inhibition (72h-ERC50) was beyond the range tested, i.e. exceeded a TWA concentration of 7.5 mg/l (100% WSF, considered to be the solubility limit of this test item under test conditions).
The EC50 for yield inhibition (72h-EYC50) was 4.7 mg/l with a 95% confidence interval ranging from 4.2 to 5.4 mg/l.
The 72h-NOEC and EC10 for growth rate inhibition were 2.5 mg/l and 3.4 mg/L, respectively . The 72h-NOEC and EC10 for yield inhibition were 1.3 mg/l and 1.2 mg/L, respectively.
The preferred observational end point in this study is algal growth rate inhibition because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1) and classification shall be based on the ErC50 (EU CLP regulation No 1272/2008 and its adaption 286/2011). In terms of the NOEC and EC10 values, there is a preference for use of the EC10 value because it is derived from the dose response curve. In contrast the NOEC depends on the experimental design (e.g. the concentrations used in the test). Thus the 72-h EC50 and EC10 based on growth rate are used for classification purposes.
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