[29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]iron

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 1 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. DMSO used as a solvent, because of limited solubility of tested sample. Chemical was tested at 0, 0, 1.2, 2.4, 5, 10 and 20 mg/l nominal concentrations. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 4.43 mg/L on the basis of mobility inhibition effects in a 48 hour study.

 

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.

 

Additional information

Short term toxicity to aquatic invertebrate:

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 1 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. DMSO used as a solvent, because of limited solubility of tested sample. Chemical was tested at 0, 0, 1.2, 2.4, 5, 10 and 20 mg/l nominal concentrations. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 4.43 mg/L on the basis of mobility inhibition effects in a 48 hour study.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to determine the freshwater algal growth inhibition test on the growth of Desmodesmus subspicatus with the test substance. Test was conducted according to OECD Guideline 201. The stock solution 20 g/l was prepared by dissolving dark blue powder in DMSO. Test solutions 21 mg/l were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. It was not possible to test at higher concentration because of formation of precipitation. Test conducted at limit test concentration 21 mg/l. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. Based on the growth rate inhibition of Desmodesmus subspicatus due to the exposure of chemical for 72 hrs, 1.6 % inhibition was observed at 21 mg/l limit concentration after the exposure of 72hrs.

 

On the basis of all above effects and results, it was observed that the chemical was insoluble in water and thus the study performed by dissolving chemical in vehicle and limit test were performed at higher solubility level. Thus by conseding the criteria of insolubility chemical cannot be consider to be toxic and not classified as per the CLP classification criteria.