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EC number: 247-825-4 | CAS number: 26586-02-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiments were carried out between 1991-06-20 and 1992-05-14.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study. Only 4 strains of S. typhimurium tested.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Principles of method if other than guideline:
- There was the following deviation from the requirements of the above mentioned principles:
- Analytical investigation (stability of the test substance in DMSO and aqua dest) was not carried out.
The Ames-standard plate test (SPT) was carried out according to:
- Ames BN et al (1973). Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci USA 70: 2281-2285; and
- Ames BN et al (1975). Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res 31: 347-364.
The Ames-preincubation test (PIT) was carried out according to:
- Yahagi T et al (1977). Mutagenicities of N-nitrosoamines in Salmonella. Mutat Res 48: 121-130; and
- Matsushima T et al (1980). Factors modulating mutagenicity in microbial tests. In: Short-Term Test Systems for Detecting Carcinogens. Edited by Norpoth KH and Garner RC. Springer Verlag Berlin, Heidelberg, New York.
The Liquid suspension assay (LSA) was carried out according to:
- Rannug U et al (1976). The mutagenicity of chloroethylene oxide, chloroacetaldehyde, 2-chloroethanol and chloroacetic acid, conceivable metabolites of vinyl chloride. Chem Biol Interaction 12: 251-263; and
- Lutz D et al (1982). Structure-mutagenicity relationship in alpha,beta-unsaturated carbonylic compounds and their corresponding allylic alcohols. Mutat Res 93: 305-315 - GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (E)-3-formylbut-2-enyl acetate
- EC Number:
- 247-825-4
- EC Name:
- (E)-3-formylbut-2-enyl acetate
- Cas Number:
- 26586-02-7
- Molecular formula:
- C7H10O3
- IUPAC Name:
- (2E)-3-methyl-4-oxobut-2-en-1-yl acetate
- Details on test material:
- - Name of test material (as cited in study report): C5-Acetat
- Physical state: colourless liquid
- Analytical purity: 98.8%
- Impurities (identity and concentrations): no data
- Lot/batch No.: no data; date of manufacturing: 1991-04-05
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions: the stability of the test substance throughout the study period was verified analytically by reanalysis
- Storage condition of test material: room temperature (N2 conditions)
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S-9
- Test concentrations with justification for top dose:
- 4 - 5000 µg/plate (SPT)
4 - 1000 µg/plate (PIT)
4 - 1250 µg/plate (LSA)
For details, see freetext. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene, N-methy-N'-nitro-N-nitrosoguanidine, 4-nitro-o-phenylenediamine, 9-aminoacridine chloride monohydrate, crotonaldehyde, methylvinyl ketone
- Remarks:
- Positive control substances depended on the tester strain and test conditions.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation; in suspension
Five independent experiments were carried out; see freetext.
NUMBER OF REPLICATIONS: 3 plates per dose and control
DETERMINATION OF CYTOTOXICITY
- Method: reduced his- background growth, decrease in the number of his+ revertants - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfil the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 500 µg/plate; see additional information on results
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 500 µg/plate; see additional information on results
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 500 µg/plate; see additional information on results
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: The test substance was completely soluble; no precipitation was noted in any experiment under any test condition.
ADDITIONAL INFORMATION ON CYTOTOXICITY:
A bacteriotoxic effect was observed at doses >= 1500 µg/plate (SPT; all tester strains), at 1000 µg/plate (PIT; TA 1537 and TA 98) and at doses >= 500 µg/plate (LSA; TA98). - Remarks on result:
- other: other: all strains (SPT with and without S-9; PIT with and without S-9; LSA with S-9);strains TA 1535 and TA1537 (LSA without S-9)
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No increase in the number of revertants was observed for:
· SPT without S-9; all tester strains
· SPT with S-9; all tester strains
· PIT without S-9; all tester strains
· PIT with S-9; all tester strains
· LSA without S-9; tester strains TA 1535 and TA 1537
· LSA with S-9; all tester strains.
Controversial results were observed in the LSA without S-9 for TA 98 (slightly enhanced figures at 500 - 1000 µg/plate; factor 1.8-2.0; this could not be confirmed by a second experiment).
Positive reaction was observed in the LSA without S-9 for TA100 from about 250 µg/plate (factor 1.8) onward with an increase in the number of mutant colonies by a factor of 8.1 at 1250 µg/plate.
Positive controls gave the expected results.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
other: negative in the standard Ames Test( Standard Plate Test and in the Preincubation Test); positive in the modified Ames Test (Liquid Suspension Assay)
According to the authors, the test substance is mutagenic in a modified Ames test (liquid suspension assay) under the experimental conditions chosen.
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