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EC number: 445-760-8 | CAS number: 122886-55-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30/10/2002-30/10/2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (1984)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- the test solutions at 31.6, 100, 316 and 1000 mg/L were prepared by direct addition of the test item in the test vessels, followed by agitation of these solutions for a few minutes.
The test solution of 10 mg/L was prepared by diluting a stock (suspension in water) of 20 mg/L with dechlorinated water. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Origin: water treatment plant of Evreux
- Laboratory culture: before the test, the inoculum was maintained under agitation for one day.
- Method of cultivation: 50 ml of sewage feed were added per L, just before agitation
- Preparation of inoculum for exposure: the inocolum was maintained in the dark, at temperatures between 21 and 23 deg C. The aeration was performed at a rate of 0.5 to 1 L/min and the pH was adjusted to 6.0- 8.0 using NaHCO3 or HCl.
-Preparation: the inocolum was left to settle, the supernatant rejected, filtered and washed with dechlorinated water. The quantity of the suspended matter was 4g/L +/- 10%
- Initial inoculum concentration in test: 1.6 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 +/- 20 mg/L Ca CO3
- Test temperature:
- 21 -23 deg C
- pH:
- 6.0-8.0
- Dissolved oxygen:
- air inserted at a rate of 0.5 to 1 L/min
- Nominal and measured concentrations:
- Nominal
10; 31.6; 100; 316; 1000 mg/L
Measured
analysis not performed - Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Type: not reported
- Material, size, headspace, fill volume: not reported
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 1.6 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated water
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 3h-EC50 (oxygen concentration)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
- Range finding study is the final study - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: yes
- As the respiration rate of the highest tested concentration (1000 mg/L) was equivalent to the respiration rate of the controls, the oxygen consumption of the four other test concentrations was not determined - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: inhibition of respiration; EC50 8.63 mg/L, 95% C.L. (7.17-10.2 mg/L) - Validity criteria fulfilled:
- yes
- Remarks:
- The difference between the two control respiration rates was ≤ 15% (0%) and the EC50 value of the positive reference substance 3,5-dichlorophenol was between 5 and 30 mg/L (8.63 mg/L).
- Conclusions:
- Under the conditions of the current test, KY-EU has no effect on the microorganisms of a water treatment plant.
NOEC = 1000 mg/L
EC50 > 1000 mg/L - Executive summary:
An activated sludge respiration inhibition test was carried out according to OECD 209 (1984) and GLP.
Nominal concentrations of 10, 31.6, 100, 316 and 1000 mg/L were tested. As the respiration rate of the highest tested concentration (1000 mg/L) was equivalent to the respiration rate of the controls, the oxygen consumption of the four other test concentrations was not determined.
No effects were observed. Therefore, it was concluded that the NOEC was 1000 mg/L and the EC50 value was > 1000 mg/L.
The validity criteria were met and the study is considered to be reliable without restrictions.
Reference
Description of key information
Under the conditions of the current test, KY-EU has no effect on the microorganisms of a water treatment plant.
NOEC = 1000 mg/L
EC50 > 1000 mg/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
An activated sludge respiration inhibition test was carried out according to OECD 209 (1984) and GLP.
Nominal concentrations of 10, 31.6, 100, 316 and 1000 mg/L were tested. As the respiration rate of the highest tested concentration (1000 mg/L) was equivalent to the respiration rate of the controls, the oxygen consumption of the four other test concentrations was not determined.
No effects were observed. Therefore, it was concluded that the NOEC was 1000 mg/L and the EC50 value was > 1000 mg/L.
The validity criteria were met and the study is considered to be reliable without restrictions.
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