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EC number: 205-860-2 | CAS number: 156-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- one eye washed after 20 seconds
- GLP compliance:
- yes
Test material
- Reference substance name:
- trans-dichloroethylene
- EC Number:
- 205-860-2
- EC Name:
- trans-dichloroethylene
- Cas Number:
- 156-60-5
- Molecular formula:
- C2H2Cl2
- IUPAC Name:
- (1E)-1,2-dichloroethene
- Details on test material:
- -Purity: 99.64%
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Age at study initiation: Young adult
- Weight at study initiation: 2806 and 3289 grams
- Housing: housed singly in suspended, stainless steel, wire-mesh cages
- Diet: Purina Certified Rabbit Chow® #5322 (ad libitum)
- Water: ad libitum
- Acclimation period: approximately 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): targeted for a temperature of 20°C ± 2°C
- Humidity (%): 50% ± 10%
- Photoperiod (hrs dark / hrs light): 12-hour light/12-hour dark cycle
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The left eye of each rabbit was not treated and served as a control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.01 mL aliquot - Duration of treatment / exposure:
- single exposure
- Observation period (in vivo):
- 1 and 4 hours and 1, 2, and 3 days following administration
- Number of animals or in vitro replicates:
- 2
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): one rabbit had eyes washed
- Time after start of exposure: 20 seconds
SCORING SYSTEM: Draize
TOOL USED TO ASSESS SCORE: biomicroscope, fluorescein, Hemastix® reagent strips
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: discharge
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 3
- Max. score:
- 3
- Reversibility:
- fully reversible
- Remarks on result:
- other: unwashed
- Irritation parameter:
- iris score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 1
- Max. score:
- 2
- Reversibility:
- fully reversible
- Remarks on result:
- other: washed eye
- Irritation parameter:
- conjunctivae score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 2
- Max. score:
- 3
- Reversibility:
- fully reversible
- Remarks on result:
- other: washed eye
- Irritation parameter:
- chemosis score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 3
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: washed eye
- Irritation parameter:
- other: discharge
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 3
- Max. score:
- 3
- Reversibility:
- fully reversible
- Remarks on result:
- other: washed eye
- Irritation parameter:
- cornea opacity score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 4
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: washed eye
- Irritation parameter:
- cornea opacity score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Remarks on result:
- other: unwashed
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 1 Hour
- Score:
- 0
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 4 Hours
- Score:
- 0
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 24 h
- Score:
- 0
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 48 h
- Score:
- 0
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 72 h
- Score:
- 0
- Irritation parameter:
- iris score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 1
- Max. score:
- 2
- Reversibility:
- fully reversible
- Remarks on result:
- other: unwashed
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 1 Hour
- Score:
- 1
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 4 Hours
- Score:
- 1
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 24 h
- Score:
- 0
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 48 h
- Score:
- 0
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 72 h
- Score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 2
- Max. score:
- 3
- Reversibility:
- fully reversible
- Remarks on result:
- other: unwashed
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 1 Hour
- Score:
- 1
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 4 Hours
- Score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 24 h
- Score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 48 h
- Score:
- 1
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 72 h
- Score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- other: range
- Time point:
- other: 1 hour to 72 hours
- Score:
- >= 0 - <= 2
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: unwashed
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 1 Hour
- Score:
- 2
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- other: 4 Hours
- Score:
- 2
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 24 h
- Score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 48 h
- Score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- Unwashed Eye
- Time point:
- 72 h
- Score:
- 0
- Irritant / corrosive response data:
- The test substance produced severe corneal opacity in the washed eye. In addition, it produced moderate iritis and conjunctival redness and copious blood-tinged discharge (confirmed with Hemastix® reagent strips) in both treated eyes, and moderate and mild chemosis in the washed and unwashed eye, respectively. Fluorescein stain examinations were positive for corneal injury in the washed eye and negative in the unwashed eye. Biomicroscopic examinations revealed moderate to severe corneal injury in the washed eye and no corneal injury in the unwashed eye. The treated eyes of both rabbits were normal by 3 days after treatment.
Any other information on results incl. tables
Unwashed Rabbit Eye |
|||||
Time |
1 Hour |
4 Hours |
1 Day |
2 Days |
3 Days |
Corneal Opacity |
0 |
0 |
0 |
0 |
0 |
Iris |
1 |
1 |
0 |
0 |
0 |
Redness |
1 |
2 |
2 |
1 |
0 |
Chemosis |
2 |
2 |
0 |
0 |
0 |
Discharge |
3(H+) |
2(H+) |
0 |
0 |
0 |
Biomic of Cornea |
- |
- |
0 |
0 |
0 |
H+ = Hemastix® evaluation positive for blood. |
|||||
Fluorescein examinations: negative for corneal injury at all intervals |
Washed Rabbit Eye |
|||||
Time |
1 Hour |
4 Hours |
1 Day |
2 Days |
3 Days |
Corneal Opacity |
1 |
2 |
4 |
1 |
0 |
Iris |
1 |
1 |
1 |
0 |
0 |
Redness |
1 |
2 |
2 |
1 |
0 |
Chemosis |
3 |
3 |
1 |
1 |
0 |
Discharge |
3(H+) |
3(H+) |
0 |
0 |
0 |
Biomic of Cornea |
- |
- |
4 |
2 |
0 |
H+ = Hemastix® evaluation positive for blood. |
|||||
Fluorescein examinations: positive for corneal injury 1 day after treatment. |
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
Under the conditions of this study, the test item was an eye irritant. - Executive summary:
The test substance was evaluated for acute eye irritation potential in 2 young adult New Zealand white rabbits. An aliquot of 0.01 mL of the test substance was administered to 1 eye of each animal. The eyes of one rabbit were washed following treatment. The untreated eye served as a control. The conjunctiva, iris, and cornea of each treated eye were evaluated and scored according to the Draize scale approximately 1 and 4 hours and 1, 2, and 3 days following administration.
The substance produced severe corneal opacity in the washed eye. In addition it produced moderate iritis and conjunctival redness and copious blood-tinged discharge (confirmed with Hemastix® reagent strips) in both treated eyes, and moderate and mild chemosis in the washed and unwashed eye, respectively. Fluorescein stain examinations were positive for corneal injury in the washed eye and negative in the unwashed eye. Biomicroscopic examinations revealed moderate to severe corneal injury in the washed eye and no corneal injury in the unwashed eye. The treated eyes of both rabbits were normal by 3 days after treatment. Under the conditions of this study, the test item was an eye irritant.
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