[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test performed according to relevant guideline and compliant to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

EC Number:

248-236-5

EC Name:

[29H,31H-phthalocyaninetrisulphonyl trichloridato(2-)-N29,N30,N31,N32]copper

Cas Number:

27121-30-8

Molecular formula:

C32H13Cl3CuN8O6S3

IUPAC Name:

Copper, [29H,31H-phthalocyanine-C,C,C-trisulfonyl trichloridato(2-)-kN29,kN30,kN31,kN32]-

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling
- Representative sample of total water amount 10L: 20mL
- Appearance of sample : blue solution
- Temperature of water samples during the toxicity testing 22°C+1°C
- 0h, 48h, 96h: one control and two treatment samples respectively

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was weighed into a beaker. Water for dilution was added and the mixture homogenized using an Ultra-Turrax and poured into the chamber under stirring with a glass rod. The chamber contents were then stirred for approximately 30 minutes with a KPG-stirrer and a glass rod. All tested concentrations were present as a blue solution. Slight particulate matter was observed on the bottom of the test chamber.

In order to determine the substance concentration, water samples were taken from the middle of the test chamber by pipetting approx. 20 mL into a screw-neck glass bottle at study start, and after 48 and 96 hours.

The following values were determined during the study:
Nominal concentration: 100 mg/L
Measured concentrations after 0h: 105 mg/L, 98.3 mg/L
Measured concentrations 48h: 101 mg/L, 105 mg/L
Measured concentrations 96h: 103 mg/L, 99.8 mg/L

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Strain: Brachydanio rerio (HAMILTON-BUCHANAN)
- Source: breeding station Corporate Toxicology (Hoechst AG)
- Age at study initiation (mean and range, SD): appr. 7 months
- Length at study initiation (length definition, mean, range and SD): the body length of 7 representative fish from the batch was measured: 2.8 - 3.2 cm (mean 3.0, SD 0.14)
- Food type: Tetra Min, Tetra Werke, Melle
- Frequency: twice daily ad libitum

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test): 22 ± 1 °C, light period 12 h daily, oxygen content > 80 % of the saturation value, population density < 1 g fish/L water (200 L vessel)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

The total hardness of the dilution water was determined weekly and was 2.3 mmol [Ca(2+) + Mg(2+)] / L during the study.

Test temperature:

Concentration group: 21.4 - 22.0
Control group: 21.4 - 22.0

pH:

Concentration group: 7.8 - 8.2
Control group: 7.9 - 8.2

Dissolved oxygen:

[mg/L]
Concentration group: 7.4 - 7.8
Control group: 7.9 - 8.3

Nominal and measured concentrations:

Nominal concentration: 100 mg/L (corresponds to 41,5 mg/L registration substance and 58.5 mg/L water)
Measured concentrations after 0h: 105 mg/L, 98.3 mg/L
Measured concentrations 48h: 101 mg/L, 105 mg/L
Measured concentrations 96h: 103 mg/L, 99.8 mg/L

All measured concentrations were in a range of ± 10 % of the theoretical value.

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L glass chambers, 30 cm x 22 cm x 24 cm

- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water accordiing to ISO/DIS 7346/1, aeration until oxygen saturation, pH 8.5

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: 700 lux

After the test concentrations had been prepared and water parameters recorded, 7 fish were assigned to each test and control chamber. The fish received no feed for the entire study period. Inspection of the fish took place after 3, 6, 24, 48, 72 and 96 hours and involved recording the lethality and visible changes in appearance and behavior. Dead fish were removed from the chambers. Fish were considered dead when there was a lack of opercular movement and no response to slight mechanical stimulus. The water parameters were measured and recorded before study start and after 0, 24, 48, 72 and 96 hours.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (corresponds to 41.5 mg/L registration substance and 58.5 mg/L water

Details on results:

Lethality:
In this 96-hour acute toxicity study in zebra fish (Brachydanio rerio) lethality occurred neither in the control nor in the 100 mg/L-group .
The LC50 after 96 hours therefore exceeds 100 mg/L

Appearance and behavior:
No changes in appearance and behaviour could be observed, but due to the blue colour of the solution observations were disturbed.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A 96h acute toxicity test with Brachydanio rerio had been performed as a limit test (poor solubility of test item) according to OECD 203 and compliant to GLP. LC50 (96h) > 100 mg/L nominal (corresponds to 41.5 mg/L registration substance and 58.5 mg/L water).

Executive summary:

When testing the lethal effect of the test item in zebra fish (Brachydanio rerio) over a total of 96 hours, no lethality occurred at 100 mg/L (nominal) whichcorresponds to 41.5 mg/L registration substance and 58.5 mg/L water. The LC50 after 96 hours therefore exceeds 100 mg/L. The nominal concentration tested was 100 mg/L and a negative control (0 mg/L). No changes in behaviour and appearance could be observed, but the observation was disturbed by the blue colour of the test item solution. The substance concentration was determined in the 100 mg/L group. The measured concentration values were within a range of ± 10 % of the theoretical value.