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A mixture of isomers of: 1,1'-[(3,5(or 2,4 or 4,6 or 2,6)-dihydroxy-o(or m or p)-phenylene)bis(azo-meta-phenyleneazo{1-[3-(dimethylamino)propyl]-1,2-dihydro-6-hydroxy-4-methyl-2-oxopyridine-5,3-diyl})]dipyridinium-dichloride-dihydrochloride; 1-(1-[3-(dimethylamino)propyl]-5-{3-[x-(4-{1-[3-(dimethylamino)propyl]-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-5-pyridinio-3-pyridylazo}phenylazo)-2,4(or 2,6 or 3,5 or 4,6)-dihydroxyphenylazo]phenylazo}-1,2-dihydro-6-hydroxy-4-methyl-2-oxo-3-pyridyl)pyridinium-dichloride-dihydrochloride (where x is variable)
EC number: 404-540-1 | CAS number: 159405-95-5 BRAUN HM 2763; BROWN HM 2763; BRUN HM 2763; BRUNO HM 2763
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
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- Nanomaterial surface chemistry
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- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 (96h) = 115 mg/l, corresponding to 143 mg/l as nominal concentration.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 115 mg/L
Additional information
Stability of test item after 96 h in water was less than 80 %. Results were calculated on the basis of 80 % relative to the nominal concentrations of the test substance, instead of geometric mean of actually analysed concentrations as proposed in the test guideline. The test result still is considered to reflect the real situation with an appropriate approximation, thus the study all in all is considered as reliable with restrictions.
After 1.5 hours of exposure all fish had died at nominal 560 mg/l and after 6.5 hours 9 out of 10 fish had died at nominal 320 mg/l. At 96 hours of exposure 100 % mortality was seen at nominal 320 mg/l, 70 % at 180 mg/l and 10 % at 100 mg/l and no mortality at the other concentrations and the blank.
Analysis of the samples taken at the start (t = 0) revealed actual concentrations of 78 to 86 % at the start of the exposure. During the exposure period the actual test concentration decreased below 80 % relative to the nominal concentration. At 32 and 100 mg/l this decrease was 37 % and 28 %, respectively, possibly due to the active uptake and elimination of test substance by the fish exposed.
All results were related to actual concentrations of 80 % relative to the nominal concentrations.
Under test conditions, the test substance induces mortality at actual 80 (nominal 100) mg/l and higher, with an actual (nominal) 96h-LC50 of 115 (143) mg/l.
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