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EC number: 204-857-3 | CAS number: 127-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from authoritative databases and secondary source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
- Qualifier:
- according to guideline
- Guideline:
- other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
- Principles of method if other than guideline:
- Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Purity: 97.8%
- Water solubility: 27.7 g/100 g (20°C) - Radiolabelling:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Length at study initiation (lenght definition, mean, range and SD): 8
- Sex: MF - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 6 wk
- Test temperature:
- 25°C
- pH:
- 6.0-8.5
- Details on test conditions:
- RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: TLm(48h) > 1000 mg/L, Rice fish (Oryzias latipes) - Nominal and measured concentrations:
- Nominal concentrations:
1st concentration area: 1 mg/l (1 ppm (w/v))
2nd concentration area: 0.1 mg/l (0.1 ppm (w/v)) - Reference substance (positive control):
- not specified
- Key result
- Conc. / dose:
- 1 mg/L
- Temp.:
- 25 °C
- Type:
- BCF
- Value:
- > 0.5 - < 1.6 L/kg
- Basis:
- whole body w.w.
- Time of plateau:
- 6 wk
- Remarks on result:
- other: pH ranges from 6-8.5, respectively
- Key result
- Conc. / dose:
- 0.1 mg/L
- Temp.:
- 25 °C
- Type:
- BCF
- Value:
- <= 5 L/kg
- Basis:
- whole body w.w.
- Time of plateau:
- 6 wk
- Remarks on result:
- other: pH ranges from 6-8.5, respectively
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively.
- Executive summary:
Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical.The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" andother guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively.Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/l and 0.1 mg/l, respectively. Range finding study involve theTLm(48h) > 1000 mg/LonRice fish(Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.
Reference
Bioaccumulation test results:
|
2 W |
3 W |
4 W |
6 W |
|
1st Concentration area
|
Test substance concentration (ppm (w/v)) |
0.943 |
0.908 |
0.869 |
0.859 |
Bioconcentration factor (L/kg) |
<= 0.5 |
<= 0.5 |
<= 0.5 |
<= 0.5 |
|
<= 0.5 |
<= 0.5 |
<= 0.5 |
<= 0.5 |
||
2nd Concentration area
|
Test substance concentration (ppm (w/v)) |
0.087 |
0.085 |
0.088 |
0.085 |
Bioconcentration factor (L/kg) |
<= 5 |
<= 5 |
<= 5 |
<= 5 |
|
<= 5 |
<= 5 |
<= 5 |
<= 5 |
Description of key information
Bioaccumulation study from authoritative database (J-CHECK & BCF database, 2017 and EnviChem, 2014) and secondary source (IUCLID dataset, 2000) was conducted on test organism Cyprinus carpiofor 6 weeks for evaluating the bioconcentration factor (BCF value) of Sodium 3-nitrobenzene sulphonate(CAS no. 127-68-4).The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" andother guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively.Cyprinus carpio (length - 8 dimensionless) was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/land 0.1 mg/l, respectively. Range finding study involve theTLm(48h) > 1000 mg/Lon Rice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance Sodium 3 -nitrobenzene sulphonate on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the chemical Sodium 3-nitrobenzenesulphonate is not expected to bioaccumulate in the food chain.
Key value for chemical safety assessment
- BCF (aquatic species):
- 0.5 L/kg ww
Additional information
Experimental study and predicted data for the target chemical and various supporting studies for its structurally similar read across substances were reviewed for the bioaccumulation end points which are summarized as below:
In an experimental key study from authoritative database (J-CHECK & BCF database, 2017 and EnviChem, 2014) and secondary source (IUCLID dataset, 2000) for the target chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical .The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/land 0.1 mg/l. Range finding study involve the TLm(48h) > 1000 mg/L on Rice fish(Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively.
From CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application) V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical), the bioaccumulation i.e BCF for test chemical was estimated to be 5.05 dimensionless . The predicted BCF result was based on the 5 OECD principles.
In a next weight of evidence study from authoritative database (J-CHECK, 2017) for the test chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish) and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 0.5mg/land 0.05 mg/l, respectively. Analytical method involve the recovery ratio: Test water: Fish: 91.0 %, - Limit of quantitation: Test water: 1st concentration area : 0.018 mg/L, 2nd concentration area : 0.0018 mg/L, Fish : 0.20 microg/g. Range finding study involve theLC50 (48h) 326 mg/L on Rice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 4.1% at the start of exposure. The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be ≤ 0.4 L/Kg at a conc. of 0.5 mg/l and ≤ 4.1 L/Kg at a conc. of 0.05 mg/l, respectively.
In another weight of evidence study for test chemical, the bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical (J-CHECK, 2017). Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1mg/land 0.1 mg/l, respectively. Range finding study involves the TLm (48 hr) ˃ 1000 mg/l (w/v) on Rice fish (Oryzias latipes). The bioconcentration factor (BCF value) test chemical on Cyprinus carpio was determined to be in the range of 0.2-0.5 L/Kg at a conc. of 1 mg/l and ≤ 2 L/Kg at a conc. of 0.1 mg/l, respectively.
On the basis of above results for target chemical (from authoritative J-CHECK, BCF database, EnviChem, secondary source & modelling database CompTox Chemistry Dashboard,2017) it can be concluded that the BCF value of test chemical ranges from 0.5–5.05 which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.
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