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EC number: 200-589-6 | CAS number: 64-67-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: No LD50 or NOEC determined because only 1 concentration was tested. Very few details are available in this short note.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- MUTATION STUDIES WITH DIETHYL SULFATE (DES)
- Author:
- Corcos A
- Year:
- 1 981
- Bibliographic source:
- Arabidopsis inf serv 18: 150-152
- Reference Type:
- publication
- Title:
- Further mutation studies with Diethyl sulfate (DES)
- Author:
- Corcos A
- Year:
- 1 982
- Bibliographic source:
- Arabidopsis inf serv 19: 123
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Exposure of seeds to one concentration of DES and the growth and survival of the plants are followed up to the production of seeds and germination of this second generation.
In the second publication, the same study was repeated with 0.15% of DES. - GLP compliance:
- not specified
Test material
- Reference substance name:
- Diethyl sulphate
- EC Number:
- 200-589-6
- EC Name:
- Diethyl sulphate
- Cas Number:
- 64-67-5
- Molecular formula:
- C4H10O4S
- IUPAC Name:
- diethyl sulfate
- Details on test material:
- no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
Test organisms
- Species:
- other: Arabidopsis thaliana
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: thale cress
- Plant family: Brassicaceae
- Variety: Columbia
- Source of seed: no data
- Prior seed treatment/sterilization: no data
- Historical germination of seed (germination of seed lot tested): no data
- Seed storage: no data
Study design
- Test type:
- other: whole life cycle experiment
- Study type:
- laboratory study
- Substrate type:
- not specified
- Limit test:
- no
- Remarks:
- no data
Results and discussion
Effect concentrationsopen allclose all
- Species:
- other: Arabidopsis thaliana
- Dose descriptor:
- other: effect at the only concentration tested
- Effect conc.:
- 0.5 other: %
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: germination, growth, flowering, seed production and germination of the M2 generation
- Species:
- other: Arabidopsis thaliana
- Dose descriptor:
- other: effect at the only concentration tested
- Effect conc.:
- 0.15 other: %
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: seed germination of the M2 generation
Any other information on results incl. tables
Germination was not affected by the treatment since 111 out of 114 seeds germinated (97.3%). Among the control, 40 out of 40 germinated.
The control plants flowered 19 days after germination. So did 38 of the treated plants (33.3% of the treated seeds), however, 71 (62.3% of the treated seeds) did not flower by that time and 5 had died (4%).
The control plants produced seeds 56 days after germination. So did the 38 non delayed plants. Among the delayed ones, 24 had flowered by that time (33.8% of the delayed plants), but the plants were small. The other plants died without flowering (66.2% of the delayed plants).
Germination of the M2 plants has been seriously affected by the treatment with DES (Table 1). The size of the M2 plants was in general smaller than the controls. Only among the progeny of M1 plants 4,5,and 6 were normal sized plants.
Table 1: Germination results of M2 plants (exposure at 0.5%)
M1 |
numbers |
% |
Plant 1 |
13 out of 40 |
32.5 |
Plant 2 |
15 out of 40 |
37.5 |
Plant 3 |
15 out of 40 |
37.5 |
Plant 4 |
11 out of 40 |
27.5 |
Plant 5 |
27 out of 40 |
64.5 |
Plant 6 |
25 out of 40 |
62.5 |
Table 2: Germination results of M2 plants (exposure at 0.15%)
M1 |
numbers |
% |
Plant 1 |
8 out of 20 |
40 |
Plant 2 |
3 out of 10 |
30 |
Plant 3 |
9 out of 20 |
45 |
Plant 4 |
4 out of 20 |
20 |
Plant 5 |
4 out of 20 |
20 |
Plant 6 |
6 out of 20 |
30 |
Plant 7 |
5 out of 20 |
25 |
Plant 8 |
4 out of 20 |
20 |
Plant 9 |
4 out of 20 |
20 |
Plant 10 |
7 out of 24 |
29 |
Plant 11 |
6 out of 43 |
14 |
Plant 12 |
1 out of 10 |
10 |
Plant 13 |
6 out of 36 |
16 |
Plant 14 |
23 out of 43 |
53 |
Plant 15 |
4 out of 14 |
28 |
Plant 16 |
4 out of 9 |
44 |
Plant 17 |
3 out of 7 |
42 |
Plant 18 |
1 out of 6 |
16 |
Plant 19 |
6 out of 18 |
33 |
Total |
108 out of 333 |
32 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- Not a standard study
- Conclusions:
- The drenching of seeds in DES solution at 0.5% induced effects on survival, plant growth, flowering time and seed production and germination of the second generation. The repetition of the study with 0.15% had the same effect on the germination of the M2 plants.
- Executive summary:
Seeds of Arabidopsis thaliana were exposed to DES (0.5%) and then grown. Survival, germination, growth, flowering time and seed production were determined. Seeds from some of these plants were then reexposed to DES and germination was determined.
DES produced effects on survival, plant growth, flowering time and seed production of the M1 generation and also decreased the germination rate of M2 generation (average germination: 44%).
The repetition of the study with 0.15% had the same effect on the germination of the M2 plants (32%).
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