Methanesulphonic acid

Endpoint summary

Additional information

In vitro:

In two Ames tests the Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538 were exposed to methanesulfonic acid concentrations of 100, 500, 1000, 2500 or 5000 µg/plate (USEPA 2003) and 10, 32, 100, 316, or 1000 µg/plate (Pennwalt 1989), respectively, in the presence and absence of metabolic activation. In both assays there was no increase in revertant colonies seen up to the cytotoxicity threshold of 5000 and 1000 µg/plate methanesulfonic acid, respectively. The extent of response to the positive controls was not indicated in any study. These findings were supported by another Ames test (Zeiger 1989) using S. typhimurium strains TA100 and TA1535. Futhermore, methanesulfonic acid was tested in a modification of the Ames test (McMahon et. al. 1979) in various Salmonella typhimurium and E. coli strains including E. coli WP2 and WP2 uvrA-. According to the publication, methanesulfonic acid belonged to negative substances in this modified Ames Test. In a gene mutation HPRT test (BASFAG 2010) performed according to OECD guideline 476 and GLP, the test substance did not lead to a biologically relevant increase in the number of mutant colonies either without S9 mix or after the addition of a metabolizing system in two experiments performed independently of each other. The mutant frequencies at any concentration were within the range of the concurrent negative control values and within the range of our historical negative control data.

Combining all data, Methanesulfonic acid was not mutagenic in any of the reported in vitro assays.

 

In vivo:

In a mouse micronucleus assay (Pennwalt 1989), 5 male and female mice each were administered 0, 20, 100 or 500 mg/kg bw methanesulfonic acid by a single oral gavage. Three males at the 500 mg/kg bw dose had to be excluded from the analysis because two of them died and one was sacrificed due to its general condition. Surviving high-dose males showed piloerection, one male and one female each exhibited rales. Bone marrow samples were taken 24, 48 or 72 hours after treatment. The positive control produced a significant increase in micronucleated polychromatic erythrocytes. The test substance did not induce micronuclei in bone marrow erythrocytes. Methanesulfonic acid did not induce chromosomal aberrations in the Mammalian Erythrocyte Micronucleus Test. Additionally, in a Drosophila SLRL test (Roehrborn 1958) performed comparable to the later published OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster), no mutagenic effects were observed.

Short description of key information:
Methane sulfonic acid induced negative results in ames assay, in-vitro gene mutation (HPRT) assay, in-vivo micronucleus assay and Drosophila SLRL test.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

EU classification according to Annex I of Directive 67/548/EEC: no classification required GHS classification (GHS UN rev.3, 2009): no classification required