Methyl chloroformate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study, however only 4 strains tested

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

4 strains only

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroformate

EC Number:

201-187-3

EC Name:

Methyl chloroformate

Cas Number:

79-22-1

Molecular formula:

C2H3ClO2

IUPAC Name:

methyl chloroformate

Details on test material:

- Name of test material (as cited in study report): chloroformic acid methyl ester
- Physical state: colorless liquid
- Analytical purity: 99.2 %
- Lot/batch No.: 2/i v Mai 87
- Storage condition of test material: 4°C

Method

Target gene:

his +/-

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium TA 98, 100, 1535, 1537

Metabolic activation:

with and without

Metabolic activation system:

S9 -mix

Test concentrations with justification for top dose:

0.0005 µl - 5.0 µl/Platte (TA 1535); 0.0005 - 1.0 µl/Platte (TA 100, 1537, 98), respectively 0.5-5000 µg/Platte (TA 1535); 0.5 - 1000µg/Platte (TA 100, 1537, 98)

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: ethanol
- Justification for choice of solvent/vehicle: stability and soloubility

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 2- amino anthracene

Remarks:

all strains with s9 mix

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: TA 100; TA 1535: N-methyl-N'-nitro-N-nitrosoguanidine; TA 98 : 4-nitro-o-phenylendiamine; TA 1357 : 9-aminoacridine

Remarks:

with out S-9 mix

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation); preincubation

DURATION
- Preincubation period: 20 min
- Exposure duration: 48-72 h

DETERMINATION OF CYTOTOXICITY
- Method: relative total growth

Evaluation criteria:

The test chemical is considered positive in this assay if the following criteria are met:

In general, the test is considered as positive if the following requirements are fulfilled:
 
Doubling of the spontaneous mutation rate (control)
Dose response relationship
Reproducibility of the results

Statistics:

mean + sd

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Raw data see attached file.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative