Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 627-132-7 | CAS number: 1227096-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of green algae
- Data waiving:
- other justification
- Justification for data waiving:
- other:
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 2016
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identification: Amines, di-C16-C18-alkyl methyl
Abbreviation: DAM M2HT
Chemical name: Amines, di-C16-C18-alkyl methyl
IUPAC name: N-C16-18 (even numbered)-alkyl-N-methyl, C16-18 (even
numbered)-alkyl-1-amine
CAS Number: 1227096-04-9
EC Number: 627-132-7
Batch: 100002434128
Purity: 100% UVCB
Water content: 0.03%
Physical state / Appearance: White solid paste
Expiry date: 16 February 2025
Storage conditions: Room temperature in the dark - Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Test organism: Raphidocelis subcapitata strain CCAP 278/4 were obtained from the Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland.
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- OECD TG 201 medium
- Limit test:
- no
- Total exposure duration:
- 72 h
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1990-07-31 to 1990-09-13
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- no data
- Vehicle:
- no
- Details on test solutions:
- A stock suspension of 1020 g/L was prepared by dispersing the test substance in deionized water (50°C) with an ultrasonic processor.
The selected test concentrations were prepared by dilution of this stock suspension in the inoculated test medium. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: ATCC 22662
ACCLIMATION
- Conditions: maintained on mineral salts agar slants in the light at room temperature. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- no data
- Test temperature:
- 22.5 - 23.0°C
- pH:
- 6.8 - 6.9 at the beginning of the test and 7.2 - 8.3 at the end of the test
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 0.01 - 0.03 - 0.09 - 0.27 - 0.81 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100-ml erlenmeyers containing 40 ml of mineral salts medium.
TEST MEDIUM / WATER PARAMETERS
- Conductivity: < 5 µS/cm
- Copper: < 0.01 mg/L
OTHER TEST CONDITIONS
- Illumination: continuous uniform illumination, provided in the spectral range of 400 to 700 nm.
- Light intensity: in the range of 6000 to 10000 lux.
- Continuous rotation of the culture flasks at 100 rpm to prevent sedimentation of the algae.
TEST CONCENTRATIONS
- Range finding study: in the range finding test, at nominal concentrations of 0.1 - 1 - 10 and 100 mg/L, growth of the algae is inhibited. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate, purity 95%
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL = 0.10 - 0.14 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.05 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL = 0.04 - 0.06 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.01 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- Cell concentrations were determined spectrophotometrically.
- Results with reference substance (positive control):
- potassium dichromate: ErC50 = 0.79 mg/L and EbC50 = 0.20 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- Validity: the test is valid.
- ErC50 and EbC50 of the reference compound, potassium dichromate (0.79 and 0.20 mg/L respectively).
- Increase of the extinction of the control by a factor 98 (must be at least a 16 fold increase).
- Deviation of no more than 1.5 pH unit.
Deviations:
- 1 minnor deviation: the KH2PO4 and NaHCO3 concentrations in the test medium were increased to 160 and 100 mg/L, respectively, to maintain a constant pH. - Executive summary:
Acute toxicity of Amines, bis(hydrogenated tallow alkyl) methyl to Selenastrum capricornutum has been investigated through OECD Guideline 201.
Selenastrum capricornutum were exposed for 72 hours to a solution of Amines, bis(hydrogenated tallow alkyl) methyl
at different nominal concentrations of 0.01 - 0.03 - 0.09 - 0.27 and 0.81 mg/L.ErC 50-72h determined was of 0.12 mg/L, EbC50-72h of 0.05 and NOEC of 0.01 mg/L.
Validity criteria were fulfilled.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.12 mg/L
- EC10 or NOEC for freshwater algae:
- 0.01 mg/L
Additional information
Three references have been identified for assessing impact of N-C16-18 (even numbered)-alkyl-N-methyl, C16-18 (even numbered)-alkyl-1-amine on algae. As for invertebrates, none of them are considered as Klimish 1 due to the absence of analytical confirmation of the exposure concentrations. The one which has been retained followed the OECD 201 requirements and did not use solvent for solubilizing the test item. Being the most compliant, this test was identified as a key study (Kroon, 1990) and resulted in a 72h ErC50 for algae of 0.12 mg/L.
A further algae study with Amines, C16-18-alkylmethyl is currently running and the proposed draft report is planned to receive the beginning of July 2023. The final report is expected to be available at the end of August 2023.
This information will be submitted a little bit later based on ECHA communication/decision number CCH-D-2114585941-37-01/F.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.