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EC number: 459-290-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2005 january 21st to 2005 january 26th
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study performed according to the OECD Guideline 202.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Sample Reference No. : DEV75332
Appearance : Tan powder
Aqueous solubility : < 0.05 mg/L
Structure confirmation : Confirmed to be consistent with the proposed structure
Purity determination : 98.8 wt.%
Stability : Stable
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organisms were cultured within the testing facility. An organism origination
statement and taxonomic verification are located in a central file maintained by the
Ecotoxicology group, Room 2020, Building 320, Kodak Park. The following taxonomic
characterization applies to the daphnids:
Phylum - Arthropoda
Class - Crustacea
Order - Cladocera
Family - Daphnidae
Genus - Daphnia
Species - magna (Straus)
The test organisms are cultured in 100-L stainless steel tanks provided with a continuous
flow of filtered-treated-tempered water. The water depth is maintained by use of an
overflow pipe that discharges into a floor drain. The tanks are continuously aerated by
passing oil-free filtered air through air stones. The culturing facility is maintained at a
constant temperature of 20°C and is illuminated with fluorescent lighting for 16 hours
followed by a 30-minute transition period leading to 8 hours of darkness.
The daphnids are fed an ample amount of spinach-yeast-fish food slurry that may be
supplemented with a yeast-Cerophyll leaves-trout chow (YCT) mixture or green algae,
such as Selenastrum capricornutum. The dietary components of the YCT are analyzed
routinely to identify contaminants that could interfere with the outcome of the study. The
findings of these analyses are located in a central file maintained by the Ecotoxicology
group. No known contaminants at concentrations that could interfere with the outcome of
studies have been identified.
The colonies are observed daily and maintenance is performed as necessary. Logbooks
are maintained that document organism feeding, behavioral observations,
physio-chemical parameters and maintenance activities.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- The temperature of the test substance solutions ranged from 19.6 to 19.7°C.
- pH:
- The pH of the test substance ranged from 8.2 to 8.3.
- Dissolved oxygen:
- The dissolved oxygen concentrations of the test substance solutions ranged from 9.1 to 9.3 mg/L.
- Nominal and measured concentrations:
- Exposure solutions containing the test substance at nominal concentrations of 0.1 mg/L
were tested. The exposure solutions were prepared by direct addition of a stock solution
containing the test substance to test media. Two test substance stock solutions were
prepared by weighing approximately 25.1 and 25.6 mg of test substance into weigh boats
and transferring it into separate 25-mL volumetric flasks. The flask was then brought to
volume with a solvent, N,N-dimethyl formamide (DMF). The 0.1-mg/L test substance
exposure solutions were prepared by pipetting 2 mL of stock solution into 22-L mixing
vessels containing 20 L of laboratory dilution water. Carrier solvent control solutions
were also prepared by pipetting 2 mL of DMF into 20 L of laboratory dilution water. The
solutions were stirred for approximately 2 hours using stir plates and Teflon-coated stir
bars. A 200-mL aliquot was removed from the middle of each mixing vessel’s water
column and transferred into the 250-mL test vessels. - Details on test conditions:
- Study Observations
The physical parameters of the exposure solutions were measured at test start and test
end.
The appearances of the exposure solutions were observed and recorded at test start, after
4 hours of exposure and at the end of each 24-hour period.
The test organisms were observed for behavioral abnormalities at test start, after 4 hours
of exposure and at the end of each 24-hour period.
Collection of Samples for Concentration Verification
Samples of the controls and test substance exposure solutions were analyzed at test start
and test end to verify test substance concentration. The samples analyzed at test start
were collected from approximately mid-depth of the 22-L mixing vessels using a
spigot-valve assembly. The samples analyzed at test end were poured from the 250-mL
test vessels into the sample containers. Samples were analyzed using HPLC/UV.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.11 mg/L
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.11 mg/L
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Conclusions:
- The results of this study indicate that the 48-hour EC50 value is greater than the measured
aqueous solubility of the substance. Based on this test, the test substance would not be classified
according to the European Union’s labeling directive [ l l ] and would correspond to a “low
concern level” according to the U.S. EPA’s assessment criteria [12].
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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