Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-471-4 | CAS number: 60-34-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 09 June 2010 to 07 September 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: compliant to GLP and testing guideline (the deviation was not considered to have compromised the validity of the study); adequate coherence between data, comments and conclusions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2020
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- pH of mineral medium was not recorded at the beginning of the test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- as above
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Methylhydrazine
- EC Number:
- 200-471-4
- EC Name:
- Methylhydrazine
- Cas Number:
- 60-34-4
- Molecular formula:
- CH6N2
- IUPAC Name:
- methylhydrazine
- Details on test material:
- - Name of test material (as cited in study report): mono methyl hydrazine
- Substance type: monoconstituent
- Physical state: slightly yellow liquid
- Analytical purity: 99.3%
- Impurities (identity and concentrations): monomethylamaine : 0.5%, water: 0.3%
- Purity test date: 09 December 2009
- Lot/batch No.: 09TL120001
- Expiration date of the lot/batch: 01 January 2013
- Storage condition of test material: at room temperature and protected air (under nitrogen gaz)
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sampled from a water treatment plant receiving sewage from a predominantly domestic origin (collected from: the water treatment plant of Evreux (France).
- Storage conditions: 22°C +/- 2°C
- Preparation of inoculum for exposure: the inoculum contained approximately 11 x 104 bacteria per mL (determination with a Malassez cell counter). In order to obtain a final concentration of approximately 104 bacteria per liter in all test suspensions, inoculum was diluted 20-fold with mineral medium and 1.81 mL/L of (diluted) inoculum was added to each test vessel.
- Pretreatment: the inoculum was prepared by initially removing the biggest particles and seiving accross a filter paper (porosity 4-7 µm). In order to wash out the dissolved organic carbon (DOC) and to lower the carbon organic content, the inoculum was preconditioned for 5 days before use for the study. Air was bubbled through the inoculum during this preconditioning period. The inoculum was seived again accross a filter paper after the acclimation period.
- Concentration of sludge: 104 bacteria per liter in all test suspensions
- Initial cell/biomass concentration:
- Water filtered: yes/no - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 0 mg/L
- Based on:
- test mat.
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Initial conc.:
- 4 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: reconstituted water (OECD and EEC recommended) was prepared using deionized water and analytical grade reagents (see section "any other information on matherials and methods")
- Test temperature: between 20°C and 24°C
- pH: was not recorded at the beginning of the test
- Aeration of dilution water: at least 20 minutes and standing at test temperature between 20 and 24 hours before the beginning of the test
- Continuous darkness: yes
SAMPLING
- no samples were taken at day 1 and day 4 for chemical analysis (see datails on analytical methods)
CONTROL AND BLANK SYSTEM
- Inoculum blank: one group containing the inoculum
- Toxicity control: one group containing the test item (at 2 mg/L), the reference item (sodium acetate at 3 mg/L) and inoculum, and one group containing the test item (at 4 mg/L), the reference item (sodium acetate at 3 mg/L) and inoculum.
- Other: procedure control: one group containing the reference item (sodium acetate at 3 mg/L) and inoculum.
Reference substance
- Reference substance:
- acetic acid, sodium salt
- Remarks:
- at 3 mg/L
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 1 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 6.2
- Sampling time:
- 4 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 12
- Sampling time:
- 8 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 23
- Sampling time:
- 16 d
- Remarks on result:
- other: test substance at 4 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 44.9
- Sampling time:
- 28 d
- Remarks on result:
- other: test substance at 4 mg/L
- Details on results:
- For the test suspension at 2 mg/L:
No significant inhibition of the inoculum due to toxicity of the test item was noted since the toxicity control reached 45.4% of the ThOD after 14 days.
The 10-day window (the 10 days immediately following the attainment of 10% biodegradation) of the test item started on the 5th day. Biodegradation totalled 32% (mean of the two replicates) at the end of this 10-day window on the 15th day.
However for this test suspension at 2 mg/L biodegradtion values were considered like not relevant since the difference between the values of two replicates was too important and results at the end of the test day 25 and 29 were not relevant either.
Therefore results of this test concentration of 2 mg/L are not taking into account to assess the readily biodegradation of the test item. (However values are presented in the results of this report because results showed tendency of the test item to be not readily biodegradable).
For the test suspension at 4 mg/L:
No significant inhibition of the inoculum due to toxicity of the test item was noted since the toxicity control reached 39.7% of the ThOD after 14 days.
The 10-day window (the 10 days immediately following the attainment of 10% biodegradation) of the test item started on the 6th day. Biodegradation totalled 23% and 44.9% over the test period (mean of the two replicates) at the end of this 10-day window on the 16th day. Biodegradation in this control totalled 77.8% after 28 days.
BOD5 / COD results
- Results with reference substance:
- Day 1 : 0% biodegradation
Day 4: 52.1% biodegradation
Day 8: 66.5% biodegradation
Day 18: 71.6% biodegradation
Day 29: 77.4% biodegradation
Any other information on results incl. tables
All validity criteria were respected for the test concentration at 4 mg/L:
. dissolved oxygen depletion in the inoculum blank did not exceed 1.5 mg/L after 28 days,
. dissolved oxygen was >= 0.5 mg/L in all test suspension replicates during the test,
. biodegradation values of test item replicates deviated by less than 20% at the end of the test,
. biodegradation in the reference test was 71.6% after 14 days then it was at least 60% within this period,
. biodegradation in the toxicity control was 39.7% (based on ThOD) after 14 days then it was at least 25% within this period.
All validity criteria concerning inoculum blank, reference test and the toxicity control were respected as indicated above. However, for the test concentration at 2 mg/L:
. dissolved oxygen was < 0.5 mg/L in the test suspension replicates at day 29,
. biodegradation values of test item replicates at the end of the test deviated by more than 20%.
Such difference at the end of the test can occurred when the concentration of test item was low because the precision of the method is lesser in these conditions.
It was assumed that the invalidated criteria for the test concentration at 2 mg/L were not sufficient to question this study since all validity criteria concerning inoculum blank and reference test and the test concentration at 4 mg/L were respected.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable, but biodegradable to some extent
- Conclusions:
- The biodegradation of Mono Methyl Hydrazine reached 23% at the end of the 10-day window (day 16) and 44.9% at the end of the test (day 28).
Under the experimental conditions of the study, the test item Mono Methyl Hydrazine was therefore not readily biodegradable. - Executive summary:
Methods
Six groups (two replicates per group) were used to determine the Biological Oxygen Demand (BOD) evolved by the degradation of the test item:
. one group containing the inoculum (inoculum blank),
. one group containing the test item (at 2 mg/L) and inoculum (test suspension),
. one group containing the test item (at 4 mg/L) and inoculum (test suspension),
. one group containing the reference item (sodium acetate at 3 mg/L) and inoculum (procedure control),
. one group containing the test item (at 2 mg/L), the reference item (sodium acetate at 3 mg/L) and inoculum (toxicity control),
. one group containing the test item (at 4 mg/L), the reference item (sodium acetate at 3 mg/L) and inoculum (toxicity control).
The test and reference items were dissolved in reconstituted water (OECD mineral medium) prepared from deionized water with a conductivity < 10 µS/cm.The mineral medium was prepared the day before the beginning of the study. It was maintained under aeration for at least 20 minutes and standing at test temperature between 20 and 24 hours before the beginning of the test.
The inoculum consisted of secondary effluent sampled from a sewage treatment plant and then aeratedfor 5 days. Inoculum was added to the test suspensions in order to obtain approximately 104bacteria per liter in each group.
BOD flasks were filled to the brim and closed until O2 measurement.
The dissolved oxygen was measured in duplicate (two replicates per group) at the beginning of the test, every 3 or 4 days thereafter and at the end of the test, for each group.
Chemical analysis
As the test item was not found to be readily biodegradable in the worst case,i.e.taking onto account theThOD NH3, chemical analysis was not carried out in order to verify that the O2consumption was not due to nitrification.
Results
Validity criteria
All validity criteria were respected for the test concentration at 4 mg/L:
. dissolved oxygen depletion in the inoculum blank did not exceed 1.5 mg/L after 28 days,
. dissolved oxygen was >= 0.5 mg/L in all test suspension replicates during the test,
. biodegradation values of test item replicates deviated by less than 20% at the end of the test,
. biodegradation in the reference test was 71.6% after 14 days then it was at least 60% within this period,
. biodegradation in the toxicity control was 39.7% (based on ThOD) after 14 days then it was at least 25% within this period.
All validity criteria concerning inoculum blank, reference test and the toxicity control were respected as indicated above. However, for the test concentration at 2 mg/L:
. dissolved oxygen was < 0.5 mg/L in the test suspension replicates at day 29,
. biodegradation values of test item replicates at the end of the deviated by more than 20%.
Such difference at the end of the test can occurred when the concentration of test item was low because the precision of the method is lesser in these conditions.
It was assumed that the invalidated criteria for the test concentration at 2 mg/L were not sufficient to question this study since all validity criteria concerning inoculum blank and reference test and the test concentration at 4 mg/L were respected.
Test item biodegradation
The reference item degraded normally under the test conditions.
For the test suspension at 2 mg/L
No significant inhibition of the inoculum due to toxicity of the test item was noted since the toxicity control reached 45.4% of the ThOD after 14 days.
The 10-day window (the 10 days immediately following the attainment of 10% biodegradation) of the test item started on the 5th day. Biodegradation totalled 32% (mean of the two replicates and graphically determined) at the end of this 10-day window on the 15th day.
However for this test suspension at 2 mg/L biodegradation values were considered like not relevant since the difference between the values of two replicates was too important and results at the end of the test day 25 and 29 were not relevant either.
Therefore results of this test concentration of 2 mg/mL are not taking into account to assess the readily biodegradation of the test item. (However values are presented in the results of this report because results showed tendency of the test item to be not readily biodegradable)
For the test suspension at 4 mg/L
No significant inhibition of the inoculum due to toxicity of the test item was noted since the toxicity control reached 39.7% of the ThOD after 14 days.
The 10-day window (the 10 days immediately following the attainment of 10% biodegradation) of the test item started on the 6th day. Biodegradation totalled 23% and 44.9% over the test period (mean of the two replicates and graphically determined) at the end of this 10-day window on the 16th day. Biodegradation in this control totalled 77.8% after 28 days.
Conclusion
The biodegradation of Mono Methyl Hydrazine reached 23% at the end of the 10-day window (the 10 days immediately following the attainment of 10% biodegradation) on the 16 day and 44.9% at the end of the test.
Under the experimental conditions of the study, the test item Mono Methyl Hydrazine was therefore not readily biodegradable in the 28-day closed bottle test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.