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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: detailed report, incl. all relevant information; GLP; vehicle not reported

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
3-hydroxy-2'-methyl-2-naphthanilide
EC Number:
205-205-0
EC Name:
3-hydroxy-2'-methyl-2-naphthanilide
Cas Number:
135-61-5
Molecular formula:
C18H15NO2
IUPAC Name:
3-hydroxy-N-(2-methylphenyl)naphthalene-2-carboxamide
Constituent 2
Reference substance name:
Naphtol AS-D
IUPAC Name:
Naphtol AS-D

Method

Test concentrations with justification for top dose:
1 Experiment: 4 - 10000 µg/plate
2 Experiment: 4 - 5000 µg/plate
Vehicle / solvent:
no data
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: several, related to strain and presence of S9mix
Details on test system and experimental conditions:
METHOD OF APPLICATION: in agar (plate incorporation);
DURATION no data


NUMBER OF REPLICATIONS: 3 * 2
DETERMINATION OF CYTOTOXICITY
- Method: other:desity of bacterial lawn was estimated microscopically
Evaluation criteria:
increase of revertant colonies
Statistics:
no data

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Positive controls validity:
valid
Species / strain:
E. coli WP2 uvr A
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

Naphtol AS-D was not mutagenic in the Ames Test