1,4-Benzenedisulfonic acid, 2,2'-[1,2-ethenediylbis[(3-sulfo-4,1-phenylene)imino[6-[bis(2-hydroxypropyl)amino]-1,3,5-triazine-4,2-diyl]imino]]bis-, sodium salt (1:6)

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate

EC Number:

240-245-2

EC Name:

Disodium 4,4'-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)amino]stilbene-2,2'-disulphonate

Cas Number:

16090-02-1

IUPAC Name:

disodium 2,2'-ethene-1,2-diylbis{5-[(4-anilino-6-morpholin-4-yl-1,3,5-triazin-2-yl)amino]benzenesulfonate}

Details on test material:

- Name of test material (as cited in study report): FAT-65'023/N
- Batch No.: Lab.No. EBT 474/BSG
- Expiration date: December 1995
- Purity: According to the sponsors files
- Physical state: solid
- Appearance: beige powder
- Stability: guaranteed by the sponsor

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Farm-Research, D-7867 Wehr/Baden, West-Germany
- Age at test initiation: Adult mature with clitellum ( > 2 months of age )
- Weight at test initiation: From 300 to 360 mg average weight

ACCLIMATION
- Acclimation period: 24 h in soil used for the test

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 ± 1 °C

pH:

5.5 - 5.6

Moisture:

At the start of exposure: 33 - 40 % of dry weight of soil
At the end of exposure: 30 - 38 % of dry weight of soil

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1 liter beakers, covered with perforated plastic film
- Amount of soil or substrate: 750 g of soil (wet weight)
- No. of organisms per container: 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- Composition of artificial substrate: According to guideline

OTHER TEST CONDITIONS
- Photoperiod: Continous illumination
- Light intensity: 400 - 500 lux of cold white fluorescent light

EFFECT PARAMETERS MEASURED: Mortality, Flaccidity

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3

Nominal and measured concentrations:

Nominal: 1.37, 4.1, 12.3, 37, 111, 333 and 1000 mg test substance / kg (dry weight of soil)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

1.37 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1.37 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Any other information on results incl. tables

Nominal conc.(mg/l)	Mortality (cumulated) after
	7 days		14 days
	Quantity	%	Quantity	%
0	0	0	0	0
1.37	0	0	0	0
4.1	0	0	2	5
12.3	0	0	1	2.5
37.0	0	0	2	5
111	0	0	1	2.5
333	0	0	2	5
1000	0	0	6	15