1,3,6-Naphthalenetrisulfonic acid, 7-[2-[2-[(aminocarbonyl)amino]-4-[[4-chloro-6-[[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]phenyl]diazenyl]-, sodium salt

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 April 2007 to 23 August 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

air comparison pycnometer (for solids)

Type:

relative density

Density:

1.54 g/cm³

Temp.:

24.5 °C

Two test series with different weights were performed at ambient temperature (24.5 °C).

1^strun

Weight 12.4672 g	p1 / psi	p2 / psi	Volume / cm^3	DensityρPR/ g / cm^3
1. measurement	19.575	11.268	8.0423	1.5502
2. measurement	19.540	11.245	8.0592	1.5470
3. measurement	19.540	11.241	8.0828	1.5424
Mean			8.0614	1.5465

 

2^ndrun

Weight 12.5925 g	p1 / psi	p2 / psi	Volume / cm^3	DensityρPR/ g / cm^3
1. measurement	19.608	11.263	8.1835	1.5388
2. measurement	19.627	11.275	8.1771	1.5400
3. measurement	19.514	11.208	8.1894	1.5377
Mean			8.1833	1.5388

 

Mean density ρPR for the two test series: 1.543 g/cm³

Relative density (compared to water at 4°C) D₄^R:

D₄^R= ρ_PR/ ρ_W4= 1.543 g/cm³/ 1.000 g/cm³= 1.54

Conclusions:

The relative density (compared to water at 4°C) is DR4 = 1.54

Executive summary:

The purpose of this study was the determination of relative density of the test item according to the consolidated version of Council Directive67/548/EEC Annex V (Council Directive 92/69/EEC),Part A: Methods for the determination of physico-chemical properties, A.3. Density of liquids and solids and OECD test guideline OECD 109 (1995)Density of liquids and solids.The study was performed in accordance with the Principles of Good Laboratory Practice (GLP) and reported with a valid GLP certificate.

 

The density of a solid in any form can be measured at ambient temperature (R) with the gas comparison pycnometer. Before the measurement the apparatus with test item was swept with 19.5 psi Helium for 10 times. Thereby a certain amount of gas (Helium, purity min. 99.99 %) was expanded from the volume with the test item (V1=36.31cm³)into an additional volume (V2=20.84cm³). From the pressure drop from p1 to p2 the volume of the test item can be calculated. For the calculation of density a mass measurement was taken before performing the volume measurement.

 

The relative density (compared to water at 4°C) is D^R₄= 1.54

Description of key information

The relative density (compared to water at 4°C) is D^R₄= 1.54

Key value for chemical safety assessment

Relative density at 20C:

1.54

Additional information

The relative density of the test item was determined according to methods A.3. Density of liquids and solids and OECD test guideline OECD 109 (1995) Density of liquids and solids.

The density of a solid in any form can be measured at ambient temperature (R) with the gas comparison pycnometer. Before the measurement the apparatus with test item was swept with 19.5 psi Helium for 10 times. Thereby a certain amount of gas (Helium, purity min. 99.99 %) was expanded from the volume with the test item (V1=36.31cm³) into an additional volume (V2=20.84 cm³). From the pressure drop from p1 to p2 the volume of the test item can be calculated. For the calculation of density a mass measurement was taken before performing the volume measurement.

 The relative density (compared to water at 4°C) is D^R₄= 1.54