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Diss Factsheets
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EC number: 203-148-6 | CAS number: 103-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- AN IMMUNOTOXICITY ASSESSMENT OF FOODFLAVOURING INGREDIENTS
- Author:
- C. L. GAWORSKI,
- Year:
- 1 994
- Bibliographic source:
- Fd Chem. Toxic. , 1994
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Immunotoxicity of test chemical was assessed in CD1 mice.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Phenylacetic acid
- EC Number:
- 203-148-6
- EC Name:
- Phenylacetic acid
- Cas Number:
- 103-82-2
- Molecular formula:
- C8H8O2
- IUPAC Name:
- phenylacetic acid
- Details on test material:
- - Name of test material (as cited in study report): phenylacetic acid
- Substance type: Organic
- Physical state: Solid
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Details on test animal
TEST ANIMALS
- Source: Charles River Breeding Laboratories
- Age at study initiation: 6-8 wk of age
- Weight at study initiation: no data
- Fasting period before study: no data
- Housing: group housed in polypropylene cages with hardwood chip bedding
- Diet (e.g. ad libitum): Certified Purina Rodent Chow 5002 Ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18-26°C
- Humidity (%): 10-70%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12-hr light/dark cycle.
IN-LIFE DATES: From: To: no data
Administration / exposure
- Route of administration:
- other: intragastrically
- Vehicle:
- other: 1% methyl cellulose
- Details on oral exposure:
- PREPARATION OF DOSING SOLUTIONS:
Test chemical was diluted with 1% methyl cellulose
Amount of vehicle : 10ml/kg - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 5 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 250, 500 or 1000 mg/kg bw
Basis:
- No. of animals per sex per dose:
- Total :120
0 mg/kg bw:30 females
250 mg/kg bw:30 females
500 mg/kg bw:30 females
1000 mg/kg bw:30 females - Control animals:
- yes, concurrent vehicle
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: No data
DETAILED CLINICAL OBSERVATIONS: No data
BODY WEIGHT: Yes
-observed twice each day
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study):
No data
FOOD EFFICIENCY: No data
WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): Yes / No / No data
- Time schedule for examinations:
OPHTHALMOSCOPIC EXAMINATION: No data
HAEMATOLOGY: No data
CLINICAL CHEMISTRY: No data
URINALYSIS: No data
NEUROBEHAVIOURAL EXAMINATION: No data
OTHER:
Body weights of the mice used in the PFC assay were measured at the time of dosing initiation, exposure day 5, and at autopsy. Spleen and thymus weights were measured following removal from all animals in
the PFC assay, with organ/body weight ratios calculated using individual body weights measured at the
time of autopsy. PFC assay was carried out - Sacrifice and pathology:
- GROSS PATHOLOGY: No data
HISTOPATHOLOGY: No data - Statistics:
- Dunnett's test and chi-square analysis were used to evaluate mean survival time and mortality data in the host resistance assay. For continuous response data, analysis of variance (two tailed) and appropriate post-hoe comparisons using Dunnett's test were performed on natural logarithmic or Iog it transformed PFC data, For the positive control group, post-hoc comparisons were made to the naive control group using a Student's t-test. Individual groups of data were evaluated for outliers prior to statistical analysis (Dixon, 1953)
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- Mortalities were observed at all the dose concentration
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not specified
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Gross pathological findings:
- not examined
- Neuropathological findings:
- not specified
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Other effects:
- not specified
Effect levels
- Dose descriptor:
- NOEL
- Effect level:
- 1 000 other: mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- other: no effect observed on body weight, mortality observed in all dose group, no plaque forming cells were observed
- Remarks on result:
- other: No toxic effects were observed
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- The No observed effect level (NOEL) of test chemical in CD1 rats in a 5-day study was observed at a dose concentration of 1000 mg/kg bw.
- Executive summary:
Immunotoxicity of test chemical was assessed in CD1 mice. Female CD1 mice were administered intragastrically with test chemical at a dose concentration of 0, 250 or 500, 1000mg/kg bw on a daily basis for 5 days. A host resistance assay and plaque-forming cell (PFC) response to sheep erythrocytes were carried out. The results indicated that the test chemical did not modulate the cell-mediated or humoral immune response. no cheange in body weight was observed .Hence No observed effect level (NOEL) for test chemical was considered to be 1000 mg/kg bw,.
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