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Diss Factsheets
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EC number: 202-439-5 | CAS number: 95-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: No informations on GLP and Guideline followed, but sufficiently documented in the publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3,4-xylenol
- EC Number:
- 202-439-5
- EC Name:
- 3,4-xylenol
- Cas Number:
- 95-65-8
- Molecular formula:
- C8H10O
- IUPAC Name:
- 3,4-dimethylphenol
- Details on test material:
- - Name of test material (as cited in study report): 3,4-Xylenol
- Analytical purity: > 95 %
- Other: Commercial Source, not repurified before test
Constituent 1
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- AFNOR (1974) reconstituted hard water for toxicity tests (pH, 7.8-8.2; hardness, 200mg 1-1 expressed as CaCO3).
Acetone was used as dispersent-solvent (<0.1 ml/l)
Test organisms
- Test organisms (species):
- other: Daphnia magna Straus 1820
- Details on test organisms:
- Daphnids (Daphnia magna Straus 1820) from the IRCHA Laboratory and have been cultured parthenogenetically in
the Pasteur Institute Laboratory since 1975. Daphnids were cultured in 10-1 tanks, with aerated hardwater (7.5 + 0.4mg 1-1 as Ca; 5.2 + 0.3mg 1 1 as Mg;pH = 7). The breeding room was maintained at a regulated temperature of 22 + I°C under a photoperiod of 16 h daylight/8 h darkness. The Daphnids were fed with a diet of Chlorella vulgaris (1.0 x 106 cells/daphnid/h).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Hardness:
- (7.5 + 0.4mg 1-1 as Ca; 5.2 + 0.3mg 1 1 as Mg
- Test temperature:
- 22 + I°C
- pH:
- pH = 7
- Dissolved oxygen:
- >= 25% saturation (2.27 ml-1 02 at 20°C)
- Details on test conditions:
- Observations of test populations were carried out after 24h exposure. Daphnids which were unable to swim within 15 s after stimulation by gentle agitation of the water were considered to be immobilized, even if they could still move their antennae. Dissolved oxygen, pH, and temperature were measured at the end of the toxicity tests. Percentages of immobilization (between 10 and 90% on the basis of total number of Daphnia per concentration) were recorded and plotted as a function of concentration on log-probit paper. The points obtained were fitted to a straight line from which the IC50 was read as the abscissa of the point corresponding to 50% immobilization. The results were considered valid if dissolved oxygen measured at the end of the test was at least equal to 25% saturation (2.27 ml- 1 02 at 20°C), if the percentage of immobilization observed for the controls was zero (ISO, 1980), and if the IC50 of the reference compound (potassium dichromate) ranged between 0.9 and
1.5mg 1-1 after 24h exposure. Each chemical was assayed in duplicate with a minimum of three replicates on the following days. The 24-h IC50 and 95%. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 24 h
- Dose descriptor:
- IC50
- Effect conc.:
- 0.153 mmol/L
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 0.136-0.163
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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