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Diss Factsheets
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EC number: 227-563-7 | CAS number: 5888-87-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06/2016 to 08/2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 15112601
- Expiration date of the lot/batch: Nov. 2020
- Purity test date: Nov. 2015
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room Temperature (20 ± 5°C); Keep away from humidity
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: <0.1g/L;
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not known
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any): none
- Final preparation of a solid: applied as saturated solution - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
4.6 to 100 mg/L nominal
1.3 to 35.1 mg/L measured
- Sampling method: HPLC - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A saturated solution of the test item was prepared for the test. This was done by weighing
the nominal load 100 mg/L (exact loads are given below), adding the corresponding
amount of dilution water and shaking vigorously for 24 hours. The resulting solution was
filtrated through 0.45 μm filters (Nylon). The lower treatments were prepared by diluting the
saturated solution with dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS Berlin
- Source: LAUS GmbH
- Age of parental stock (mean and range, SD): 0 to 24 hours
- Feeding during test
- Food type: green algae (Desmodesmus subspicatus)
0.15 - 0.20 mg org. carbon/animal/day, the amount of
carbon is based on photometric measurement (linear correlation
between absorption at 440 nm and measured
DOC concentration). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 267 to 356 mg CaCO3/l
- Test temperature:
- 19.4 - 22.2°C
- pH:
- 7.4 to 8.5
- Dissolved oxygen:
- 7.7 to 10.8 mg/l
- Nominal and measured concentrations:
- 4.6 to 100 mg/L nominal
1.3 to 35.1 mg/L measured - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Type (delete if not applicable): open
- Aeration: passive
- Renewal rate of test solution (frequency/flow rate): three times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Deionised water with an enrichment of certain minerals was used in the test. The minerals
which are added comply with the composition M4 (full composition see annex 5, page 42)
derived by ELENDT as stated in the OECD-Guideline.
OTHER TEST CONDITIONS
- Adjustment of pH: not required
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: 1000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
reproduction rate
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2.2
- Test concentrations:
4.6 to 100 mg/L nominal
1.3 to 35.1 mg/L measured - Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 16.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Reported statistics and error estimates:
- Calculation of results was performed with the help of validated software (Microsoft Excel
®). The estimation of the biological results was accomplished using the software Tox-
Rat® Professional, version 3.2.1
The number of offspring in treatment 10 mg/L (measured concentration 3.3 mg/L) was
higher than in the control and in this treatment no mortality was observed. Therefore only
treatment 22 mg/L (measured concentration 7.7 mg/L) was used for statistical evaluation.
The details of calculation are stated in Annex 4: Statistical calculation using ToxRat® Professional
3.2.1. Biological results were stated using two significant digits. - Validity criteria fulfilled:
- yes
- Conclusions:
- In the OECD 211 study, the NOEC and the LOEC were determined to be 7.7 mg/L and 16.5 mg/L, respectively.
- Executive summary:
One valid experiment was performed. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal concentration. 10 daphnia each were exposed to the test item and the blank controls for 21 days. The offspring of the animals was counted each working day. Reproduction in the treatments 4.6 mg/L – 22 mg/L did not statistically deviate from reproduction in the blank control whereas in the treatments 46 mg/L and 100 mg/L no reproduction was observed. In the treatment 100 mg/L, until day 9 all daphnia died. In treatment 46 mg/L only 4 daphnia survived until the end of the test. The number of offspring in treatment 10 mg/L was higher than in the blank control and in this treatment no mortality was observed. Therefore, only treatment 22 mg/L was used for statistical evaluation. The content of the test item in the test solutions was determined using HPLC determination. Because of the poor solubility of the test item, the correlation between nominal and measured concentration was weak. But the analysis of the test item concentrations in the treatments before and after exposition showed sufficient stability of the test item during exposition. Therefore, the determination of the results was based on the geometric means of the measured concentrations. The following results were determined for the test item BRUGGOLEN M12: NOEC = 7.7 mg/L LOEC = 16.5 mg/L
Reference
Reproduction
SD = standard deviation; RSD = relative standard deviation = coefficient of variation M = mortality of parent animal
Replicate | Blank control | 4.6 mg/L | 10 mg/L | 22 mg/L | 46 mg/L | 100 mg/L |
1 | M | 115 | 125 | M | 0 | M |
2 | 119 | 117 | 90 | 106 | M | M |
3 | 125 | 78 | 116 | M | 0 | M |
4 | 138 | M | 87 | 119 | M | M |
5 | 123 | 130 | 146 | 58 | 0 | M |
6 | 84 | M | 108 | M | M | M |
7 | 114 | 115 | 135 | 84 | M | M |
8 | 116 | 44 | 101 | 105 | 0 | M |
9 | 70 | 96 | 103 | M | M | M |
10 | M | 127 | 121 | 108 | M | M |
Mean | 111.1 | 102.8 | 113.2 | 96.7 | 0 | -- |
SD | 22.6 | 29.1 | 19.0 | 22.1 | -- | -- |
RSD | 20.3 | 28.4 | 16.8 | 22.8 | -- | -- |
Summary of Fecundity
Treatment | Total Offspring | Surviving Parent Animals | Mean Offspring per Surviving Parent Animal | Inhibition of Reproduction in % |
Blank Control | 889 | 8 | 111 | 0 |
4.6 mg/L | 822 | 8 | 103 | 8 |
10 mg/L | 1132 | 10 | 113 | -2 |
22 mg/L | 580 | 6 | 97 | 13 |
46 mg/L | 0 | 4 | 0 | 100 |
100 mg/L | 0 | 0 | 0 | 100 |
Description of key information
In the OECD 211 study, the NOEC and the LOEC were determined to be 7.7 mg/L and 16.5 mg/L, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 7.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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