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EC number: 271-178-7 | CAS number: 68516-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25.05.2012 - 28.08.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - Elendt M7 medium solvent for the test item
- The test item is soluble only in concentrated sulfuric acid
- The solubility limit of the test item is 91 μg/L (i.e. 0.091 mg/L) in water. After sonication, heating and shaking the powder test item in the test medium is visually a heterogeneous suspension.
- The mixture of 10 mg/L was prepared by weighing the powder test item and transferring it into a flask by multiple washing with test medium. The mixture was sonicated for 15 min at 40°C.
- The mixture was heated and shaken at 68-70°C for 2 h (in incubator [SOP/W/75]) and then shaken at room temperature for 72 h [SOP/W/57].
- The non-dissolved part of the test concentration was separated by filtration. A sequential filtration was performed to ensure complete removal of particles from the test solution: once through 0.45 μm, once through 0.2 μm and twice through 0.025 μm pre-conditioned nitrocellulose membrane filters.
- The filtrate after the three filtration steps is the Water Accommodated Fraction (WAF) used for exposure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology
- Age of parental stock: < 24 h old
- Feeding during test: yes
- Food type: algal suspension Pseudokirchneriella subcapitata
- Amount: supplying ~ 0.2 mg organic carbon per parent Daphnia
- Frequency: daily - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Hardness:
- 185.0 - 228.6 mg CaCO3/L
- Test temperature:
- 20.3 – 23.1 ºC
- pH:
- 7.55 - 8.28
- Dissolved oxygen:
- 7.70 - 8.72%
- Nominal and measured concentrations:
- Nominal test concentrations: control and 10 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Size, fill volume: 150 ml, 50 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): complete renewal three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Conductivity: 564 - 583 μS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h darkness
- Light intensity: 609 - 741 lx - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: No toxic effects within the range of solubility.
- Details on results:
- Based on number of alive offspring, immobilization and growth (body length) of parent daphnids.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is with high probability chronically not harmful to aquatic invertebrates. No toxic effects occur at the range of the substances solubility.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 25.05.2012 - 28.08.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- see Rationale and Justification for the Analogue Read-Across Approach used for the Registration of Pigment Brown 41 (Chapter 13)
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- - Elendt M7 medium solvent for the test item
- The test item is soluble only in concentrated sulfuric acid
- The solubility limit of the test item is 91 μg/L (i.e. 0.091 mg/L) in water. After sonication, heating and shaking the powder test item in the test medium is visually a heterogeneous suspension.
- The mixture of 10 mg/L was prepared by weighing the powder test item and transferring it into a flask by multiple washing with test medium. The mixture was sonicated for 15 min at 40°C.
- The mixture was heated and shaken at 68-70°C for 2 h (in incubator [SOP/W/75]) and then shaken at room temperature for 72 h [SOP/W/57].
- The non-dissolved part of the test concentration was separated by filtration. A sequential filtration was performed to ensure complete removal of particles from the test solution: once through 0.45 μm, once through 0.2 μm and twice through 0.025 μm pre-conditioned nitrocellulose membrane filters.
- The filtrate after the three filtration steps is the Water Accommodated Fraction (WAF) used for exposure. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology
- Age of parental stock: < 24 h old
- Feeding during test: yes
- Food type: algal suspension Pseudokirchneriella subcapitata
- Amount: supplying ~ 0.2 mg organic carbon per parent Daphnia
- Frequency: daily - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Hardness:
- 185.0 - 228.6 mg CaCO3/L
- Test temperature:
- 20.3 – 23.1 ºC
- pH:
- 7.55 - 8.28
- Dissolved oxygen:
- 7.70 - 8.72%
- Nominal and measured concentrations:
- Nominal test concentrations: control and 10 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Size, fill volume: 150 ml, 50 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): complete renewal three times a week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Conductivity: 564 - 583 μS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h darkness
- Light intensity: 609 - 741 lx - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: No toxic effects within the range of solubility.
- Details on results:
- Based on number of alive offspring, immobilization and growth (body length) of parent daphnids.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is with high probability chronically not harmful to aquatic invertebrates. No toxic effects occur at the range of the substances solubility.
Referenceopen allclose all
- mortality of the parent animals in the control was 0% (should not exceed 20% at test termination)
- the mean number of live offspring produced per parent animal surviving at test termination in the control was 111.3 (should be higher or equal to 60)
- mortality of the parent animals in the control was 0% (should not exceed 20% at test termination)
- the mean number of live offspring produced per parent animal surviving at test termination in the control was 111.3 (should be higher or equal to 60)
Description of key information
The substance is with high probability chronically not harmful to aquatic invertebrates.
No toxic effects occur at the range of the substance solubility.
NOEC (21d) >= 10 mg/L (nominal) for Daphnia magna reproduction (OECD 211)
Key value for chemical safety assessment
Additional information
The long-term aquatic toxicity was investigated in a GLP guideline study according to OECD 211 for one ‘red disazo condensation pigment’ (PR221) [lnstitute of lndustrial Organic Chemistry, Branch Pszczyna, 2012]. The waterfleas (Daphnia magna) were exposed under semi-static conditions to 10 mg/L substance (nominal). Based on reproduction and mortality no effects observed during the 21 days of test duration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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