Sodium bis[2-chloro-4-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-5-hydroxybenzenesulphonamidato(2-)]chromate(1-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 March 1994 to 31 March 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: 11
- Expiration date of the lot/batch: December 1998

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability under storage conditions: December 1998
- Stability under test conditions: < 1 g/l in water

Analytical monitoring:

yes

Details on sampling:

Concentrations: The nominal concentrations tested were 4.6, 10.0, 21.0, 46.0 and 100 mg test substance/l and a control.
Concentrations in excess of 100 mg/l have not been tested.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test organism:
The study was performed with young daphnids (at the start of the test 6 - 2 4 hours old) of a clone of the species Daphnia magna Straus. The clone is bred in the laboratories of RCC under standardized conditions. The cultivation of the parental daphnids was performed in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.

- Experimental conditions:
In each test concentration and control 20 daphnids were tested, divided into four groups of five animals, each group in 125 ml test medium in a
glass-beaker (250 ml). The daphnids were not fed during the test period.
The test was performed in an air-conditioned room.
Water temperature: 21.1 - 21.2 °C during test period.
Light: 16 hours per day, approximately 200 - 600 Lux.
The daphnids have not been fed, and the test media have not been airated during the test period.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.5 mmol/l (= 250 mg/l) as CaCO3

Test temperature:

21.1-21.2 °C

pH:

Control: 8.1 (start, 0h) and 8.0 (end, 48 h)
At 100 mg/l: 7.9 (start, 0h) and 8.0 (end, 48 h)

Dissolved oxygen:

Control: 8.8 mg/l (start, 0h)
At 100 mg/l: 9.0 mg/l (start, 0h)

After 48 h:
8.7-9.0 mg/l

Salinity:

Ratio of Ca : Mg = 4 : 1; Na : K =.10 : 1

Nominal and measured concentrations:

Nominal concentration: 100.0, 46.0, 21.0, 10.0 and 4.6 mg/l
Measured concentrations:

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS:
Test water: reconstituted water: in deionized water with a conductivity lower than 0.1 µS/cm (Milli-Q water) analytical grade salts were dissolved to following nominal concentrations, and the water was aerated to oxygen saturation:
CaCl2 x 2H20: 2.0 mmol/l (= 294 mg/l)
MgS04 x 7H20: 0.5 mmol/l (= 123 mg/l)
NaHC03: 0.75 mmol/l (= 65 mg/l)
KCl: 0.075 mmol/l (= 5.8 mg/l)

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

75.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 46.0-123.1 mg/l

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Test results:
24-h EC 50: > 100 mg test substance/l
24-h EC 0: > 100 mg test substance/l

48-hour EC 50:75.3 mg test substance/l
(95 % conf. limits: 46.0 - 123.1 mg test substance/l)

48-hour EC 0: 4.6 mg test substance/l
48-hour NOEC: 4.6 mg test substance/l
48-hour EC 100: > 100 mg test substance/l
48-hour EC 90: 369 mg test substance/l
(95 % conf. limits : 126 - 1080 mg test substance/l)

Conclusions:

The 24 and 48 h EC50 of FAT 20028/D is > 100 mg/l and 75.3 mg/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l), respectively.

Executive summary:

The 48-h toxicity test was carried out to evaluate the influence of the test substance on the mobility respectively survival of daphnids. Therefore, juvenile daphnids are exposed to the test substance for 48 h, added to the water at a range of concentrations. Under otherwise identical test conditions, and an adequate range of test substance concentrations, different concentrations of the test substance result in different percentages of daphnids being no longer capable of swimming at the end of the test or being dead. The acute toxicity of the test substance FAT 20'028/D to Daphnia magna Straus was determined in a 48-h static test according to the Commission Directive 92/69/EEC, Annex Part C.2, dated December 29, 1992 and the OECD Guideline for Testing of Chemicals, Section 2, No. 202, Part I, adopted April 04, 1984. The test was performed in compliance with Good Laboratory Practice Regulations. The nominal concentrations tested were 4.6, 10.0, 21.0, 46.0 and 100 mg test substance/1 and a control. Concentrations in excess of 100 mg test substance/l have not been tested (in accordance with the Commission Directive 92/69/EEC).

The biological test results:

24 -h EC ₅₀: > 100 mg/l

24-h EC ₀: > 100 mg/l

48-h EC ₅₀:75.3 mg test substance/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l)

48-h EC ₀: 4.6 mg test substance/l

48-h NOEC: 4.6 mg test substance/l

48-h EC ₁₀₀: > 100 mg test substance/l

48-h EC ₉₀: 369 mg test substance/l (95 % conf. limits : 126 - 1080 mg test substance/l)

The analytically determined test substance concentrations in the test media varied in the range from 108.8 % to 121.0 % of the nominal concentrations. During the test period the test substance concentrations slightly decreased to 91.3 % to 110.7 % of the nominal values, obviously due to a sedimentation of the suspended test substance. However, in spite of this sedimentation the mean measured test substance concentrations still amounted from 102.2 % to 115.3 % of the nominal test concentrations. Therefore, all reported biological results are related to the nominal concentrations of the test substance.

Description of key information

The 48 h EC50 and NOEC of Acid Red 211 in an acute toxicity to Daphnia magna Straus was 75.3 mg/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l) and 4.6 mg/l, respectively. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

75.3 mg/L

Additional information

The 48-h toxicity test was carried out to evaluate the influence of the test substance on the mobility respectively survival of daphnids. Therefore, juvenile Daphnia magna are exposed to the test substance for 48 h, added to the water at a range of concentrations. Under otherwise identical test conditions, and an adequate range of test substance concentrations, different concentrations of the test substance result in different percentages of daphnids being no longer capable of swimming at the end of the test or being dead.

The acute toxicity of the test substance FAT 20'028/D to Daphnia magna Straus was determined in a 48-h static test according to the Commission Directive 92/69/EEC, Annex Part C.2, dated December 29, 1992 and the OECD Guideline for Testing of Chemicals, Section 2, No. 202, Part I, adopted April 04, 1984. The test was performed in compliance with Good Laboratory Practice Regulations.

The nominal concentrations tested were 4.6, 10.0, 21.0, 46.0 and 100 mg test substance/l and a control. Concentrations in excess of 100 mg test substance/l have not been tested (in accordance with the Commission Directive 92/69/EEC).

The biological test results:

24 -h EC₅₀: > 100 mg/l

24-h EC₀: > 100 mg/l

48-h EC₅₀:75.3 mg test substance/l (95 % conf. limits: 46.0 - 123.1 mg test substance/l)

48-h EC₀: 4.6 mg test substance/l

48-h NOEC: 4.6 mg test substance/l

48-h EC₁₀₀: > 100 mg test substance/l

48-h EC₉₀: 369 mg test substance/l (95 % conf. limits : 126 - 1080 mg test substance/l)