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EC number: 231-445-0 | CAS number: 7554-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-methylpyrazole
- EC Number:
- 231-445-0
- EC Name:
- 4-methylpyrazole
- Cas Number:
- 7554-65-6
- Molecular formula:
- C4H6N2
- IUPAC Name:
- 4-methyl-1H-pyrazole
- Details on test material:
- - Name of test material (as cited in study report): 4-Methylpyrazol
- Physical state: liquid, clear, yellow
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- To each sample of a volume of 2 mL, 5 mL internal standard solution were added.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Separately for each concentration 0.4637, 1.00, 2.15, 4.64 and 10.00 g test substance were added to the test water using an ultra-turrax stirrer.
From the beginning of preparing the test solutions until termination of placing the fish into the aquaria 20 minutes were needed.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish (Brachydanio rerio)
- Strain: HAM. and BUCH.
- Source: Charles River Aquatics, Someren, Netherlands
- Age at study initiation: about 3 months
- Length at study initiation: 3.32 (2.5 - 4.2) cm
- Weight at study initiation: 0.203 (0.10 - 0.39) g
- Food type: "TETRA MIN" standard feed for aquarium fish, TETRA - WERKE, MELLE, FRG, ad libitum; and on workdays generally additionally live artemia
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 250 mg/L (CaCO3)
- Test temperature:
- 22 °C
- pH:
- 8.0 - 8.6
- Dissolved oxygen:
- 6.9-9.1 mg/L
- Nominal and measured concentrations:
- 46.4, 100, 215, 464, 1000 mg/L (nominal)
98.3-105.1% recovered in analysis - Details on test conditions:
- TEST SYSTEM
- Test vessel: All-glass aquaria (30 x 22 x 24 cm); one aquarium for each test group
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 10 liters (water depth about 17 cm)
- Aeration: with oil-free air
- Type of flow-through (e.g. peristaltic or proportional diluter): non-chlorinated tap water, passed through a charcoal filter
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.2 g/L
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 215 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 468 - 1 010 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Reported statistics and error estimates:
- Determination or calculation of the median lethal concentration (LC50), the NOEC and the symptoms of the nominal concentrations after 1, 4, 24, 48, 72 and 96 hours, the analytical concentrations after 1, 96 and 1+96 hours and, if possible, the LC5 and the LC95 for the calculations using the probit analysis.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Measured concentration (mg/L)
Number of fish start
Number of fish dead after 96h
45.6
10
0
100
10
0
215
10
0
468
10
0
1010
10
10
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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