L-Lysine, N2,N6-bis[N-(1-oxododecyl)glutamyl]-, sodium salt (1:?)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study was conducted between 23rd April 2003 and the 25th July 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Japanese MITI guideline

Deviations:

no

GLP compliance:

yes

Remarks:

This study was conducted in compliance with Good Laboratory Practice Standards for industrial chemicals.

Test material

Test material information

Constituent 1

Reference substance name:

Pellicer

IUPAC Name:

Pellicer

Details on test material:

Chemical name: Sodium salts of condensation products of N-Iauroyl-L-glutamic acid and L-Lysine
Lot number: 10L-10
Storage conditions: In the dark and at room temperature
Stability: The test item was stable under the storage conditions as shown by thre finding that IR spectra of the test item before the experimental start and after the experimental completion were identical.
Purity: 96%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

- Source of inoculum/activated sludge:
On-site sampling carried out at 10 locations in Japan. Return sludge was collected from sewage plants and surface water and surface soil in contact with the atmosphere was collected from rivers, lakes and the sea.
Sampling sites:
Fushikogawa city sewage plant (Sapporo-shi, Hokkaido) Fukashiba industrial sewage plant (Kashima-gun, Ibaraki)
Nakahama city sewage plant (Osaka-sIu, Osaka)
Ochiai city sewage plant (Shinjuku-ku, Tokyo)
Kitakami River (Ishinomaki-shi, Miyagi)
Srunano River (Niigata-shi, Niigata)
Yoshino River (Tokushima-shi, Tokushima)
Lake Biwa (Otsu-shi, Shiga)
Hiroshima Bay (Hiroshima-shi, Hiroshima)
Dokai Bay (Kitakyushu-shi, Fukuoka)

- Preparation of activated sludge:
Activated sludge was prepared as follows to maintain its uniformity. The filtrate (5 L) of the supernatant of the activated sludge (the activated sludge as cultivated using the method described below) was cultivated about for three months and was mixed with the mixed filtrate (5 L) of the supernatant of a sludge collected newly at each location. The mixed filtrate (10 L) was aerated, after the pH value of the mixture was adjusted to 7.0 ± 1.0

- Method of cultivation:
After ceasing aeration of the sludge mixture for approximately 30 minutes, supernatant corresponding to about one third of the whole volume was removed. Dechlorinated water was added to the remaining portion so that the total volume reached 10 L. This mixture was aerated for 30 minutes or more, and then a predetermined amount of synthetic sewage was added to the mixture so that the concentration of the synthetic sewage was 0.1% in the volume of dechlorinated water added. This procedure was repeated once every day. Cultivation was carried out at 25±2°C.

- Storage conditions: 25 +/- 2°C

- Storage length: Not stated in report

- Preparation of inoculum for exposure:
Pretreatment:
Synthetic sewage was prepared as follows:
Glucose, peptone and potassium dihydrogenphosphate were dissolved in purified water to obtain 50 g/L of the solution for each component. The pH ofthe solution was adjusted to 7.0±1.0 with sodiun hydroxide.

- Concentration of sludge:
30 mg/L

- Initial cell/biomass concentration: Concentration of the suspended solids in the activated sludge was 5600 mg/l.

- Water filtered: not stated in report

- Type and size of filter used, if any: not stated in report

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

other: BOD

Details on study design:

TEST CONDITIONS
- Composition of medium: Each 3 mL of solutions A, B, C and D were made up to 1000 mL with purified water, and then the pH was adjusted to 7
- Additional substrate: not applicable
- Solubilising agent (type and concentration if used): not applicable
- Test temperature: 25 deg C +/- 1 deg C
- pH: 7
- pH adjusted: yes
- CEC (meq/100 g): not stated in report
- Aeration of dilution water: yes
- Suspended solids concentration: 30 mg/l
- Continuous darkness: not stated in report


TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus
- Number of culture flasks/concentration: three
- Method used to create aerobic conditions: Prefiltered open air was used.
- Measuring equipment: Closed system oxygen consumption measuring apparatus
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: soda lime


SAMPLING
- Sampling frequency: days 7, 14, 21 and 28
- Sampling method: not stated
- Sterility check if applicable: not applicable
- Sample storage before analysis: not stated in report


CONTROL AND BLANK SYSTEM
In one test vessel, nothing was added to the basal culture medium (the volume was less than 300 mL by the volume (1.61 mL) of activated sludge inoculated).


STATISTICAL METHODS: Not stated in report

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

No preliminary study described in report.

Test performance:

The biodegradability test was judged valid since the measurement results fulfilled all the validity criteria of the guideline.

% Degradation

Parameter:

other: BOD

Value:

83

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance):
60% degradation after 7 d
79 % degardation after 14 d
83 % degradation after 28 d (average of 3 readings for BOD)
100% degradation after 28 days by HPLC

BOD5 / COD results

Results with reference substance:

oints of degradation plot (reference substance - aniline):
64 % degradation after 7 d
676% degradation after 14 d
77 % degradation after 21 d
79 % degradation after 28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item was biodegraded by microorganisms under the test conditions of this study.

Executive summary:

The study was conducted using the MITI guidelines, and also were conducted to the OECD 301C Guideline. The study also included the determination of dissolved organic carbon by a total organic carbon analysis (TOC).

The average percentage biodegradation for all three vessels was estimated to be 83% by BOD. The test item was considered to be readily biodegradable.