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EC number: 215-951-9 | CAS number: 1459-93-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Similar to guideline protocol, not GLP
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxicity Study of Dimethyl Isophthalate in Bacterial and Mammalian Cell System
- Author:
- Chung, YS, Chol S, Hong E-K, Ryu JC, Lee E and Choi K
- Year:
- 2 007
- Bibliographic source:
- Molecular and Cellular Toxicology 3(1), 53-59, 2007.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dimethyl isophthalate
- EC Number:
- 215-951-9
- EC Name:
- Dimethyl isophthalate
- Cas Number:
- 1459-93-4
- Molecular formula:
- C10H10O4
- IUPAC Name:
- 1,3-dimethyl benzene-1,3-dicarboxylate
- Test material form:
- not specified
- Details on test material:
- purchased from the Fluka Company. No information on purity
Constituent 1
Method
- Target gene:
- histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 postmitochondrial supernatant fraction purchased from Molecular Toxicology, Inc.
- Test concentrations with justification for top dose:
- from 156.3 to 5000 µg/plate
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA 100 and TA 1535, without S9
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminofluorene
- Remarks:
- WP2 without S9
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA 98 without S9
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537 without S9
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- Remarks:
- all strains, with S9
- Details on test system and experimental conditions:
- Master strains were checked for characteristics, spontaneious reversion rate representing amino acid requirement, ampicillin resistance for R-factor and sensitivity to crystal violet for rfa membrance mutation and to UV for DNA repair.
METHOD OF APPLICATION: plate incorporation. Each of the tester strains, with or without tha addition of S9 mix, was incubated in a shaking incubator for 30 min at 37 degrees C and then the mixture was combined with top agar containing a minimal amount of histidine or tryptophan and then poured onto the surface of a sterile petri dish containing solidified bottom agar.
- Exposure duration: 48 h.
NUMBER OF REPLICATIONS: plated and assessed in triplicate
DETERMINATION OF CYTOTOXICITY:
- Method: no information on background lawn growth/survival - Evaluation criteria:
- no data
- Statistics:
- mean and standard deviation of frequency of revertants
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The frequency of revertant colonies for any of the bacterial strains, with any dose of DMIP, either with or without metabolic activation, was not increased.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
All positive and negative controls were valid.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Dimethyl isophthalate was tested for bacterial mutagenicity in a plate incorporation assay in a protocol performed similar to OECD 471. In all five strains of bacteria, there was no increased incidence of revertants. The substance is evaluated as non-mutagenic
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