Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Physical & Chemical properties

Explosiveness

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
explosive properties of explosives
Data waiving:
study technically not feasible
Justification for data waiving:
other:

Description of key information

In accordance with REACH Annex XI, section 2, a study to test for explosive properties is not required as testing is technically not possible as a consequence of the properties of the substance.


The substance, nitrile hydratase, is a substance that is handled in a state where a mixture of the enzyme and the dead bacteria that produced it is dispersed in water.


A fundamental requirement for testing the physicochemical properties of a substance, is that the substance is tested as such, excluding all solvents. However, since the higher order structure of the constituent molecules of the proposed substance (nitrile hydratase) is damaged by the removal of water, it cannot be said that the proposed substance would be correctly evaluated by performing this test after the removal of water. It should therefore be avoided.


In order to avoid deterioration it would be possible to conduct the test by leaving the substance as a suspension (ie dispersed in water). However, to do so would render the test results meaningless as the physical properties of water would greatly influence the results of such a test. As such, it would not be possible to consider the results of any such test as indicative of the physicochemical properties of the substance as such. Furthermore, as enzymes are proteins produced by fermentation, i.e. in solution, they are not associated with explosive properties. The substance, nitrile hydratase, is a substance that is handled in a state where a mixture of the enzyme and the dead bacteria that produced it is dispersed in water. A fundamental requirement for testing the physicochemical properties of a substance, is that the substance is tested as such, excluding all solvents. However, since the higher order structure of the constituent molecules of the proposed substance (nitrile hydratase) is damaged by the removal of water, it cannot be said that the proposed substance would be correctly evaluated by performing this test after the removal of water. It should therefore be avoided. In order to avoid deterioration it would be possible to conduct the test by leaving the substance as a suspension (ie dispersed in water). However, to do so would render the test results meaningless as the physical properties of water would greatly influence the results of such a test. As such, it would not be possible to consider the results of any such test as indicative of the physicochemical properties of the substance as such. As such it is technically impossible to obtain a meaningful result.

Key value for chemical safety assessment

Explosiveness:
non explosive

Additional information

Justification for classification or non-classification

In accordance with the criteria for classification as defined in Annex I, Regulation (EC) No 1272/2008, the substance does not require classification with respect to explosive properties.