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EC number: 851-152-7 | CAS number: 1374570-57-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 December 2021 - 2 February 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test vessels were 1 L cylindrical glass bottles closed with PTFE screw caps. A magnetic stirring bar was placed in each test vessel. Blank controls were prepared with 16 mL synthetic sewage feed and 234 mL test water. Test mixtures were prepared by addition of the required amounts of test item in pre-filled test vessels (with synthetic sewage feed and test water). The 3,5-DCP stock solution was diluted with test water as necessary to obtain the required test concentrations into reference mixtures after filling with synthetic sewage and water. Then, 250 mL of sludge suspension were sequentially added in all test vessels. After addition of the inoculum, the test vessels were immediately closed and the mixtures were stirred under aeration for 3 hours.
See Table 2 for information on the contents of the test vessels.
The test was carried out without adjustment of the pH.
- Test concentration separation factor: Approx 3.16
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Origin: Activated sludge was collected (13 Dec. 2021) from a wastewater treatment plant (Aureilhan, France) receiving predominantly domestic sewage.
- Pre-treatment: Upon arrival in the test facility, the sludge was sieved (using a 1 mm2 mesh sieve), washed 3 times with chlorine-free tap water and re-suspended in tap water. The activated sludge was aerated and fed with the synthetic sewage feed (50 mL per L of sludge suspension) until the next day (final test). The dry weight of the sludge suspension was determined, then the sludge was diluted in chlorine-free tap water to obtain the required sludge solids concentration of approximately 3.0 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- Temperatures recorded continuously were situated between 18.4 and 21.6°C throughout the test (average value: 20.2°C)
- pH:
- The pH of the reaction mixtures at the end of the test period ranged from 7.44 to 8.11 (see Table 3)
- Nominal and measured concentrations:
- nominal: 10, 32, 100, 316 and 1000 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All-glass bottles of approximately 1 L capacity closed with PTFE screw caps, containing 500 mL of reaction mixture. Each test vessel was uniquely identified with study code, replicate number, start time of the test and type of series.
- Test water: Deionised water, containing less than 1 mg/L DOC (Dissolved Organic Carbon); determined value: < 0.3 mg/L DOC; DOC analysis was performed in accordance with ISO 17025.
- Inoculum: The activated sludge concentration in all test, reference and blank mixtures was approx. 1.5 g/L of suspended solids.
- 6 replicates bottles containing synthetic sewage feed, test water and inoculum (blank controls)
- 3 replicates bottles per test concentration containing synthetic sewage feed, test water, test item and inoculum (test mixtures)
- 1 replicate per reference substance concentration containing synthetic sewage feed, test water, reference substance and inoculum (reference mixtures)
- No. of vessels per abiotic control (replicates): The test item was not known to have strong reducing properties, therefore it was judged not necessary to perform abiotic controls.
The content of the test vessels is given in table 2 below.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Aeration: All suspensions were aerated and stirred during the exposure period.
- Details on termination of incubation: At the end of incubation, samples were withdrawn to measure the rate of decrease of dissolved O2 in completely filled all-glass BOD (Biological Oxygen Demand) bottles of approximately 300 mL capacity.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Total oxygen uptake inhibition was determined (carbon and ammonium oxidation not separated)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Approx 3.16
- Range finding study
- Test concentrations: 10, 100, 320 and 1000 mg/L
- Results used to determine the conditions for the definitive study:
After 3 hours of exposure, no significant effect on the total respiration rate was observed at 100 mg/L, but significant effects were found at 320 and 1000 mg/L (see Table below); all validity criteria were met (CV of oxygen total respiration rate in control replicates: 2.4%; mean total oxygen uptake rate in the blank controls: 29.9 mg O2.g-1 dry weight activated sludge.h-1; EC50 of 3,5-DCP for total respiration: 15.1 mg/L).
The test item had not a greater effect on the oxidation of ammonium (RN=RT- RH) than on general heterotrophic oxidation; this is why it was decided to perform the measurement of total oxygen uptake inhibition only in the final test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 17 mg/L
- 95% CI:
- >= 11.8 - <= 22.5
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 116 mg/L
- 95% CI:
- >= 99.3 - <= 135
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium:
No
- Blank controls oxygen uptake rate: Mean oxygen uptake rate was 29.5 mg O2/g dry weight activated sludge/h, and therefore was well above the (minimum) required value of 20 mg O2/g/h.
- Coefficient of variation of oxygen uptake rate in control replicates:7.0% at the end of the definitive test (below the recommended upper limit of 30%). - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: The EC50 of 3,5-DCP was 8.2 mg/L for total respiration and thus remained within the prescribed range of 2 to 25 mg/L. - Reported statistics and error estimates:
- The statistical outputs from ToxRat are reported in Appendix IV (attached)
- Validity criteria fulfilled:
- yes
- Executive summary:
Introduction
A study was performed to assess the effects of test item N,N-dimethyldodec-9-enamide on microorganisms from activated sludge by performing a respiration inhibition test. The method followed was designed to be compliant with OECD TG 209, "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)", referenced as Method C.11 of Commission Regulation No. 440/2008.
Methods
A definitive test was performed following the results of a range-finding test. Microorganisms from activated sludge were exposed to the test item at nominal concentrations of 10, 32, 100, 316 and 1000 mg test item/L (three replicates for each concentration) and to six blank controls. Furthermore, an exposure of the test organism to chemical 3,5-dichlorophenol (3,5-DCP) was performed in order to check the sensitivity of the batch of activated sludge used on the day of exposure. All the mixtures contained an activated sludge concentration of about 1.5 g/L of suspended solids.
After 3 hours of exposure under aeration and stirring, samples of each mixture were poured into BOD bottles to continuously measure the oxygen concentration using an oxygen electrode and determine the rate of decrease of dissolved O2. The measurement of total oxygen uptake inhibition was determined only (carbon and ammonium oxidation not separated).
Results
The validity of the test was demonstrated by an oxygen uptake rate in the blank controls higher than 20 mg O2/g/h, a coefficient of variation of oxygen uptake rate in control replicates below 30% and an EC50 of 8.2 mg/L for 3,5-DCP (within the prescribed range of 2 to 25 mg/L for total respiration).
The ECx and NOEC values at the end of the test were as follows:
Parameter
Respiration rate - Critical effect and
threshold concentration (mg/L)
95% confidence limits (mg/L)
3-hour EC10
17.0
11.8 – 22.5
3-hour EC20
32.8
25.1 – 40.7
3-hour EC50
116
99.3 – 135
3-hour NOEC
10.0
-
Statistical analyses were performed by the computer program ToxRat.
Conclusion
The effect of test item N,N-dimethyldodec-9-enamide on microorganisms from activated sludge was investigated in a respiration inhibition test. Under the experimental conditions and based upon nominal concentrations, the 3-hour EC50 value was determined to be 116 mg/L.
Reference
Range-finding test results (respiration rate and inhibition at the end (3h) of the test)
Total respiration
(RT) without ATU |
Heterotrophic respiration (RH)
with ATU |
||||
Content |
Concentration (mg/L) |
Oxygen uptake rate (mg O2.g-1.h-1) |
Inhibition (%) |
Oxygen uptake rate (mg O2.g-1.h-1) |
Inhibition (%) |
Control (1) |
0 | 29.546 | - | - | - |
Control (2) | 0 | 29.969 | - | 31.122 | - |
Ref (1) | 5.00 | 19.072 | 2.896 | ||
Ref (2) | 8.40 | 29.664 | 0.917 | ||
Ref (3) | 14.21 | 13.904 | 53.557 | ||
Ref (4) | 23.81 | 6.253 | 79.115 | ||
Ref (5) | 40.02 | 4.548 | 84.809 | ||
Test item | 10.0 | 35.587 | -18.866 | 25.129 | 13.807 |
Test item | 10.0 | 34.401 | -14.904 | 26.812 | 8.034 |
Test item | 100.0 | 29.412 | 1.761 | 20.378 | 30.102 |
Test item | 100.0 | 29.459 | 1.602 | 22.333 | 23.397 |
Test item | 320.0 | 20.702 | 30.853 | 13.087 | 55.112 |
Test item | 1000.0 | 9.395 | 68.619 | 8.203 | 71.865 |
Test item | 1000.0 | 8.753 | 70.763 | 8.059 | 72.359 |
Control (3) | 0 | 29.288 | - | - | - |
Control (4) | 0 | 30.952 | - | 27.187 | - |
Activated sludge respiration inhibition test
Table 3 and Figure 1 (attached) show the responses recorded during the final test.
Table 3. Test results (oxygen uptake rate, inhibition and pH at the end of the test).
Content |
Concentration (mg/L) |
Oxygen uptake rate (mg O2/g/h) |
Inhibition (%) |
pH |
Control (1) |
0 |
27.243 | - | 7.88 |
Control (2) |
0 |
28.001 |
- |
7.80 |
Ref (1) |
5.03 |
20.125 |
31.851 |
7.89 |
Ref (2) |
8.44 |
14.804 |
49.871 |
7.82 |
Ref (3) |
14.27 |
8.303 |
71.884 |
7.94 |
Ref (4) |
23.92 |
4.505 |
84.744 |
7.93 |
Ref (5) |
40.20 |
3.119 |
89.437 |
7.97 |
Control (3) |
0 |
29.120 |
- |
7.44 |
Control (4) | 0 | 28.710 | - | 7.81 |
Test item | 11.40 | 28.183 | 4.563 | 7.72 |
Test item | 11.42 | 28.399 | 3.833 | 7.76 |
Test item | 10.92 | 29.231 | 1.017 | 7.74 |
Test item | 32.68 | 24.673 | 16.450 | 7.74 |
Test item | 32.42 | 24.721 | 16.290 | 7.74 |
Test item | 33.18 | 25.508 | 13.622 | 7.76 |
Test item | 100.66 | 13.189 | 55.338 | 7.86 |
Test item | 101.92 | 15.254 | 48.346 | 7.82 |
Test item | 100.02 | 14.874 | 49.633 | 7.85 |
Test item | 316.36 | 7.994 | 72.931 | 8.00 |
Test item | 316.34 | 6.964 | 76.418 | 8.00 |
Test item | 315.98 | 6.609 | 77.622 | 8.02 |
Test item | 1001.16 | 4.401 | 85.097 | 8.09 |
Test item | 1000.32 | 4.282 | 85.499 | 8.09 |
Test item | 998.86 | 3.458 | 88.289 | 8.11 |
Control (5) | 0 | 31.786 | - | 7.74 |
Control (6) | 0 | 32.325 | - | 7.74 |
Description of key information
The effect of test item N,N-dimethyldodec-9-enamide on microorganisms from activated sludge was investigated in a respiration inhibition test performed according to OECD TG 209. Under the experimental conditions and based upon nominal concentrations, the 3-hour EC50 value was determined to be 116 mg/L. The 3 -hour EC10 and NOEC were 17.0 and 10.0 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 116 mg/L
- EC10 or NOEC for microorganisms:
- 17 mg/L
Additional information
A study was performed to assess the effects of test item N,N-dimethyldodec-9-enamide on microorganisms from activated sludge by performing a respiration inhibition test. The method followed was designed to be compliant with OECD TG 209, "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)", referenced as Method C.11 of Commission Regulation No. 440/2008.
A definitive test was performed following the results of a range-finding test. Microorganisms from activated sludge were exposed to the test item at nominal concentrations of 10, 32, 100, 316 and 1000 mg test item/L (three replicates for each concentration) and to six blank controls. Furthermore, an exposure of the test organism to chemical 3,5-dichlorophenol (3,5-DCP) was performed in order to check the sensitivity of the batch of activated sludge used on the day of exposure. All the mixtures contained an activated sludge concentration of about 1.5 g/L of suspended solids.
After 3 hours of exposure under aeration and stirring, samples of each mixture were poured into BOD bottles to continuously measure the oxygen concentration using an oxygen electrode and determine the rate of decrease of dissolved O2. The measurement of total oxygen uptake inhibition was determined only (carbon and ammonium oxidation not separated).
The validity of the test was demonstrated by an oxygen uptake rate in the blank controls higher than 20 mg O2/g/h, a coefficient of variation of oxygen uptake rate in control replicates below 30% and an EC50 of 8.2 mg/L for 3,5-DCP (within the prescribed range of 2 to 25 mg/L for total respiration).
The ECx and NOEC values at the end of the test were as follows:
Parameter |
Respiration rate - Critical effect and threshold concentration (mg/L) |
95% confidence limits (mg/L) |
3-hour EC10 |
17.0 |
11.8 – 22.5 |
3-hour EC20 |
32.8 |
25.1 – 40.7 |
3-hour EC50 |
116 |
99.3 – 135 |
3-hour NOEC |
10.0 |
- |
Statistical analyses were performed by the computer program ToxRat.
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