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EC number: 224-226-6 | CAS number: 4253-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11/02/97 - 13/3/97
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- See principles of method
- Principles of method if other than guideline:
- According to the OECD method 301B, CO2 should be removed from the test flask after the addition of the inoculum but before the addition of the test substance. As the test substance is only sparingly soluble, it was added directly to the test flasks and dispersed into emulsion, with CO2 removed before the addition of the inoculum.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Inoculum was taken from a urban biological water treatment plant in Versailles. The inoculum was retrieved 2 days prior to test initation and aerated then centrifuged at 20°C for 20 minutes at 4000g.The final inoculated medium was diluted to a concentration of 1.5 E5 bacteria/ml.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- other: TOC
- Initial conc.:
- 45.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 82.6 mg/L
- Based on:
- other: CO2 Th
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Inocluated mineral medium containing set concentrations of test substance was kept in the dark and aerated by passing CO2 through it. The test substance (at concentrations of between 10 and 20 mg/l of COD or TOC) was degraded by micro-organisms, as their sole carbon and energy source, through organotrophic chemotherapy. The degradation was recorded by analysing the CO2 produced over the 28 days of the test. Measurements were made on days 0, 1, 4, 6, 8, 11, 14, 22, 27 and 28. The inhibitory effects of the substance were determined by measuring degradation, through CO2 release, in a flask with reference substance, test substance and inoculum. After the CO2 traps were renewed on the last day of testing, the test flasks were acidified with 1ml of concentrated hydrochloric acid in order to release the CO2, and after 24 hours, the barium hydroxide was titrated.
- Reference substance:
- other: The reference substance, sodium benzoate (57.7% C), was 3.9 mg/ml was diluted in the test medium, giving 39mgCOD or a mass of 82.5 theoretic CO2 mgCO2Th. In the inhibition control, equilavents quantities of test substance and reference were introduced, gi
- Preliminary study:
- No data reported
- Test performance:
- DEGRADATION: 13% after 28 days.
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 13
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 45
- Sampling time:
- 14 d
- Remarks on result:
- other: Reference substance in inhibition control
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 47
- Sampling time:
- 28 d
- Remarks on result:
- other: Reference substance in inhibition control
- Details on results:
- According to the OECD guideline 301, the test substance cannot be considered as readily biodegradable.
- Results with reference substance:
- No inhibition of inoculum observed.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Biodegradation was 13% after 28 days, therefore polysulfides, di-tert-butyl, is not considered readily biodegradable.
- Executive summary:
Thiebaud (1997) is a 28 day, aerobic, GLP-compliant, ready biodegradability carbon dioxide evolution test with aerobic domestic activated sludge and control groups, following OECD guideline 301B. The study is considered reliable and suitable for use as a key study for this endpoint.
Reference
Description of key information
Biodegradation was 13% after 28 days, therefore polysulfides, di-tert-bu, is not considered readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Thiebaud (1997) is a 28 day, aerobic, GLP-compliant, ready biodegradability carbon dioxide evolution test with aerobic domestic activated sludge and control groups, following OECD guideline 301B. The study is considered reliable and suitable for use as a key study for this endpoint.
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