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EC number: 410-400-0 | CAS number: 88671-89-0 SYSTHANE TECHNICAL
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-21-04 to 2003-25-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Guideline study performed to GLP with supporting analysis.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- 0 hours and 96 hours
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- The toxicity test was conducted in 20.8-L glass aquaria, each containing 15 L of test solution. One test aquarium was established for each treatment level and the controls. A 200 mg/mL primary stock solution was prepared by adding 5.2258 g of myclobutanil (4.9959 g) to 25 mL of acetone. This stock solution was observed to be clear and yellow in color following two minutes of sonication. Each solution was mixed for one minute using a laboratory stirrer. Following mixing, the 8.0 and 16 mg/L exposure solutions were clear and colorless and contained a small oily film on the water surface. The remaining exposure solutions were observed to be clear and colorless. In addition, a control aquarium was also established and maintained under the same conditions as the treatment level solutions. The aquarium contained dilution water and no myclobutanil. The definitive test was initiated when rainbow trout were impartially selected and distributed to the test vessels until each test vessel contained 10 rainbow trout. At any given time during the exposure period, the maximum organism loading concentration did not exceed 0.8 g of biomass per liter of test solution.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Mean total length = 39 mm (range: 34 to 48 mm); N =30
Mean wet weight = 0.61 g (range: 0.36 to 0.98 g); N = 30 - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- well water
- Total exposure duration:
- 96 h
- Hardness:
- The dilution water consisted of well water with a hardness of 52 mg/L as CaC03 and an alkalinity of 39 mg/Ls as CaC03.
- Test temperature:
- 13 to 14 °C
- pH:
- 7.4 to 7.5
- Dissolved oxygen:
- 81 to 91% saturation
- Conductivity:
- 160 µmhos/cm
- Nominal and measured concentrations:
- Nominal concentrations: 1.0, 2.0, 4.0, 8.0. 16.0 mg/L
Mean measured concentrations: 0.85, 1.8, 3.0, 2.9 and 6.1 mg a.s./L - Details on test conditions:
- Test system:
The toxicity test was conducted in 20.8-L glass aquaria, each containing 15 L of test solution. Exposure levels were monitoring by measuring concentrations of myclobutanil at 0 and 96 hours. Nominal concentrations were 2.0, 4.0, 8.0 and 16 mg/L. Initial measured test concentrations were 0.85, 1.8, 3.0, 3.5 and 2.2 mg/L representing 85, 92, 75, 44 and 14% of the nominal concentration, respectively. Day 4 measured concentrations were 0.84, 1.7, 2.9, 2.4, 9.9 mg/L representing 84, 85, 73, 30, 62 % of nominal concentrations, respectively.
Test dilution water:
The dilution water (well water) used during this study was from the same source as the water used to culture rainbow trout and was characterized as having total hardness and total alkalinity (as CaC03) of 52 mg/L and 39 mg/L, respectively, a pH of 7.8 and a specific conductance of 160 µmhos/cm.
Test conditions:
Each 20.8-L aquarium, constructed entirely of glass and silicone sealant, was impartially placed in a temperature-controlled water bath designed to maintain exposure solution temperatures at 14 ± 1°C. A single aquarium was established for each treatment level and the control. The test area was illuminated with fluorescent bulbs at an intensity of 80 to 100 foot-candles at the water surface. The photoperiod during the test was the same as in the culture area. Sudden transitions from light to dark and vice versa were avoided. Light intensity was measured with a General Electric type 217 light meter.
All aquaria were examined after 0, 2, 3, 6, 24, 48, 72 and 96 hours of exposure as follows: mortalities were recorded and dead fish removed, biological observations, including sublethal effects (e.g., lethargy, loss of equilibrium) of the exposed rainbow trout and observations of the physical characteristics of the test solutions (e.g., presence of precipitate, film on the solution's surface) were made and recorded. Test solution temperature was continuously monitored during the definitive test in the 2.0 mg/L treatment level using a Fisher Scientific minimum-maximum thermometer. Gentle, oil-free aeration was initiated at the 72-hour interval to raise and maintain dissolved oxygen levels at or above 60% of saturation.
Evaluation of test conditions:
The water quality parameters were unaffected by the concentrations of myclobutanil tested and remained within acceptable ranges for the survival of rainbow trout. Daily measurement of the temperature in the test solutions and continuous temperature monitoring established that the exposure solution temperature ranged from 13 to 14°C during the definitive study.
Determination of EC50 and NOEC:
Both the initial measured concentrations and the mean measured concentrations tested and the corresponding immobilization data derived from the definitive toxicity test were used to estimate the median lethal concentrations (EC50) and 95% confidence intervals. The EC50 is defined as the concentration of the test substance in dilution water which caused immobilization of 50% of the test organism population at the stated time interval. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- Following 24 and 96 hours of exposure, 70, 100 and 100% mortality was observed among fished exposed to the 3.0, 2.9 and 6.1 mg/L treatment levels (mean measured concentrations), respectively.
No mortality or sublethal effects were observed in the 0.85 and 1.8 mg/L treatment levels or the controls. - Sublethal observations / clinical signs:
Nominal Concentration mg/L Initial Measured Concentrations as mg a.s./L (% if nominal) 96-Hour Measured Concentrations as mg a.s./L (% of nominal) Mean Measured Concentrations as mg. a.s./L (% of nominal) 24-Hours Cummulative % Immobilized 96-Hour Cummulative % Immobilized Control <0.10 <.10 0 0 0 Solvent Control <.10 <.10 0 0 0 1.0 0.85 (85) 3.4 (68) 0.85 (85) 0 0 2.0 1.8 (92) 8.4 (84) 1.8 (88) 0 0 4.0 3.0 (75) 16 (81) 3.0 (74) 70 70 8.0 3.5 (44) 18 (45) 2.9 (37) 100 100 16.0 2.2 (14) 34 (43) 6.1 (38) 100 100 - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on mean measured concentrations the 96-hour LC50 value for myclobutanil and Oncorhynchus mykiss under static conditions was determined to be 2.3 mg a.s./L, and the NOEC was determined to be 1.8 mg a.s./L.
- Executive summary:
The purpose of this study was to determine the toxicity of myclobutanil based on the results (LC50) of a 96-hour static acute toxicity test using rainbow trout (Oncorhynchus mykiss). Based on the initial measured concentrations (0-hour), the 96-hour LC50 value for myclobutanil and Oncorhynchus mykiss was determined to be 2.3 mg/L, with 95% confidence intervals of 1.8 to 2.9 mg/L. The No-Observed-Effect Concentration (NOEC) was determined to be 1.8 mg/L.
Reference
Description of key information
In a GLP-study performed according to OECD TG 203, the LC50-value of of myclobutanil was revealed to be 2.3 mg/L (96-hour). Test organism was Oncorhynchus mykiss.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 2.3 mg/L
Additional information
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