[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

liquid

Details on test material:

- Physical state: Liquid
- Storage condition of test material: room temperature, darkness (Optimal temperature at 20°C)

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Strain/clone: clone 5
- Age at study initiation (mean and range, SD): < 24 hours old
- Source: LIEBE - CNRS UMR 7146 - UFR SciFA - Université de Lorraine Campus Bridoux - Bât. IBISE, 8, rue du Général Delestraint - 57070 METZ, bred in the Laboratoires des Pyrénées et des Landes.

Study design

Test type:

static

Water media type:

other: reconstituted water

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

None

Post exposure observation period:

No

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tubes
- Type (delete if not applicable): loosely capped test tubes
- Material, size, headspace, fill volume: 15 mL capacity glass tubes, filled with 9.95 mL of reconstituted water
- No. of organisms per vessel: 5 daphnids / unit
- No. of vessels per concentration (replicates): four replicate unites for each five treatment concentrations
- No. of vessels per control (replicates): 4 replicates for the water control and 4 replicates for the solvent control.
- Reference item treatment : Potassium dichromate was tested at three different concentrations and with four replicates per concentration.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: On-going

Applicant's summary and conclusion

Conclusions:

The EC50 (48h) for the immobilization of Daphnia magna by the test substance (On-going)

Executive summary:

The influence of the test substance on immobilisation of Daphnia magna was investigated according to OECD Guideline 202, with GLP statement. Daphnids were exposed to the test item under static conditions, for 48 h. The results are not yet available (Study on going).