1-Octadecanaminium,N,N-Dimethyl-N-Octadecyl-,(Sp-4-2)-[29h,31h-Phthalocyanine2-Sulfonato(3-)-N 29,N 30,N 31,N 32]Cuprate(1-)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was performed according to guideline. Restriction due to lack of analytical monitoring of test substance concentration.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- The source of test organism was activated sludge freshly obtained from municipal sewage treatment plant: Waterschap de Aa, Schijndel, The netherlands. The activated sludge was aerated for 4 h. it was then left to settle for about 1 h. the supernatant was decanted to provide a sufficient large volume for 10% inoculum for each CO2 test flask.
- Laboratory culture: NDA
- Method of cultivation: NDA
- Storage conditions: NDA
- Storage length: NDA
- Preparation of inoculum for exposure: 30 ml of the inoculum was added to the test medium present in each of the 3L test bottles. This mixture was aerated with CO2 free air for 24 h.
- Pretreatment: NDA
- Concentration of sludge: 10% inoculum for each CO2 test flask.
- Water was first purified by reverse osmosis and thereafter passed over carbon and ion-exchange cartridges.

- Laboratory culture:
- Method of cultivation:
- Storage conditions:
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment:
- Concentration of sludge:
- Initial cell/biomass concentration:
- Water filtered: yes/no
- Type and size of filter used, if any:

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

10 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS

- Composition of medium: KH2PO4, 17 mg/L; K2HPO4, 43.6 mg/L; Na2HPO4•12H2O, 134.4 mg/L; NH4CL; , 3.4 mg/L; MgSO4•7H2O, 22.5 mg/L; CaCL; 2•2H2O, 36.4 mg/L; FeCL; 3, 0.6 mg/L; (NH4) 2SO4, 40.0 mg/L;

- Test temperature: 20 ± 2 °C

- pH: 7.4

- pH adjusted: yes

- Aeration of dilution water: no

TEST SYSTEM
- Culturing apparatus: 3 liter, 4 all-glass brown colored bottles.

- Number of culture flasks/concentration: 1

- Method used to create aerobic conditions: the CO2 free air (a mixture of 20% oxygen and 80% nitrogen) was sparged through the scrubbing solutions at a constant rate of approximately 1~2 bubbles per seconds, corresponding to 50~100 ml air/min.

CONTROL AND BLANK SYSTEM:
Innoculum Blank

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

Preliminary study:

No

Test performance:

No unusual observations during the test.

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

< 10

Sampling time:

28 d

Remarks on result:

other: 20 mg/L initial concentration

Remarks:

Using these data, greater than 90% of the material will likely have degraded in less than ½ yr.

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

15.4

Sampling time:

28 d

Remarks on result:

other: 10 mg/L initial concentration

Remarks:

Using these data, greater than 90% of the material will likely have degraded in less than ½ yr.

Details on results:

Points of degradation plot (10 mg/L test substance):
0 .2% degradation after 2 d
2.4 % degradation after 5 d
3.9 % degradation after 7 d
6.5 % degradation after 9 d
8.1 % degradation after 12d
10.8 % degradation after 15 d
13.2 % degradation after 21 d
15.3 % degradation after 28 d
15.1 % degradation after 28 d
15.3 % degradation after 28 d

10 mg/L initial concentration attained 15.3 % degradation calculated from the CO2 evaluation values after 28 days.

Points of degradation plot (20 mg/L test substance):
0 .5% degradation after 2 d
1.9 % degradation after 5 d
2.8 % degradation after 7 d
4.0 % degradation after 9 d
4.0 % degradation after 12d
4.8 % degradation after 15 d
6.4 % degradation after 21 d
7.9 % degradation after 28 d
7.6% degradation after 28 d
7.9 % degradation after 28 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (20 mg/L):
8.8% degradation after 2 d
33.4 % degradation after 5 d
44.7 % degradation after 7 d
51.3 % degradation after 9 d
63.4% degradation after 12d
83.1 % degradation after 15 d
89.6 % degradation after 21 d
91.1 % degradation after 28 d
91.7 % degradation after 28 d
91.7 % degradation after 28 d
The reference substance at 20 mg/L initial concentration attained 91.7 % degradation calculated from the CO2 evaluation values after 28 days, which exceeded 60% degradation requirement to validate the test.

Any other information on results incl. tables

CO₂-evolution in the of blank control

Day	Produced CO2(mgCO2)	% degradation
2	1.29	1.3
5	0.67	2.0
7	0.10	2.1
9	0.00	2.1
12	1.59	3.7
15	3.26	6.9
21	1.65	8.6
28	1.81	10.4
28	0.00	10.4
28	0.00	10.4

 

The total CO₂evolution in the blank at the end of the test was less than 50 mg CO₂per 3 liters medium, which validated the test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test material is not readily biodegradable according to OECD Guideline 301B given that the degradation rates calculated from CO2 evolution values were less than 60 %.

Executive summary:

In an assessment of ready biodegradability study, the test material attained <10% and 15.4% biodegradation in 28 days at initial concentration of 20 mg/L and 10 mg/L, respectively. The test substance was determined to not be readily biodegradable according to OECD Guideline 301B given that the degradation rates calculated from CO₂evolution were less than 60 %.