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EC number: 308-020-4 | CAS number: 97808-97-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 May 2019 to 9 October 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Determination of the carbon content of the test item was not conducted according to GLP. The laboratory providing the analysis was accredited with DIN EN ISO/IEC 17025:2005.
Test material
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Water
- Reference substance name:
- C12-C14 alcohol
- IUPAC Name:
- C12-C14 alcohol
- Reference substance name:
- Mono (C12-C14 alkyl) phosphate ester
- IUPAC Name:
- Mono (C12-C14 alkyl) phosphate ester
- Reference substance name:
- Di (C12-C14 alkyl) diphosphate ester
- IUPAC Name:
- Di (C12-C14 alkyl) diphosphate ester
- Reference substance name:
- Tri (C12-C14 alkyl) triphosphate ester
- IUPAC Name:
- Tri (C12-C14 alkyl) triphosphate ester
- Reference substance name:
- C12-C14 alkyl pyrophosphate esters
- IUPAC Name:
- C12-C14 alkyl pyrophosphate esters
- Reference substance name:
- Ortho-phosphoric acid
- IUPAC Name:
- Ortho-phosphoric acid
- Reference substance name:
- unknown
- Molecular formula:
- unknown
- IUPAC Name:
- unknown
- Test material form:
- solid: bulk
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Constituent 5
Constituent 6
Constituent 7
Constituent 8
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 1023S17201, identification details RM1004983
- Expiration date of the lot/batch: 09 July 2020
- Purity test date: 17 April 2019
- Purity:100%
- CAS number: 68412-59-9
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Stability under test conditions: stable under normal conditions
- Solubility and stability of the test substance in the solvent/vehicle: Not soluble in water water.
- Volatility: Negligible.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): AZV Breisgauer Bucht
- Method of cultivation: collected from municipal wastewater treatment plant
- Storage conditions: Not stated
- Storage length: sampled on the 13 August 2019, test started on 15 August 2019
- Pretreatment: the sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge.
- Concentration of sludge: 5.4g/L
- Water filtered: no - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 19.5 mg/L
- Based on:
- ThCO2
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: standard mineral medium as per guideline
- Additional substrate: none
- Solubilising agent (type and concentration if used): test item was added directly to the test vessels due to the low solubility of the test substance, using inert stainless steel foil as a carrier material.
- Test temperature: 21.6 to 22.3°C
- pH: not measured
- CEC (meq/100 g): not stated
- Aeration of dilution water: 30 to 100ml/min, determined by counting air bubbles.
- Suspended solids concentration: 30 mg/l dry solids
- Continuous darkness: not stated
TEST SYSTEM
- Culturing apparatus: flasks of at least 1.5L
- Number of culture flasks/concentration: 3 flasks
- Method used to create aerobic conditions: continuous aeration with CO2 free air, kept mixed with magnetic stirrers
- Test performed in closed vessels due to significant volatility of test substance: System was stated as sealed
- Details of trap for CO2 and volatile organics if used: 47.88g of NaOH were dissolved in 6000ml deionised water in closed vessels to produce a 0.2M NaOH solution. The CO2-free air production system consisted of an air compressor, three 1000 mL gas wash bottles filled with dry soda lime in series followed by one bottle filled with 0.1 M NaOH (sodium hydroxide). At the end of the system was one gas wash bottle filled with demineralised water, followed by an empty one to catch any drops of condensation water. A colour change of the soda lime from white to blue indicates that the CO2 absorption capacity is depleted. The CO2-free air is passed on to an air distributor with two input and 22 output channels and through PE-tubes.
SAMPLING
- Sampling frequency: 0, 4, 7, 11, 14, 21, 28 days
- Sampling method: 6ml of NaOH sampled from the first of two CO2 absorber flasks connected in line and the Inorganic Carbon determined. On day 28 2ml of 4M hydrochloric acid (HCl) was added into each reactor to release the CO2 dissolved in the water. On day 29 the IC was determined in both CO2 absorber flasks in line.
- Sterility check if applicable: not stated
- Sample storage before analysis: no information provided about delay between sampling and analysis or storage
- Analysis method: Inorganic carbon was measured with total carbon analysers (TOC-L and TOC5050A, Shimadzu) by purging the inorganic carbon with H3PO4(25%) using a non dispersive infrared (NDIR) detector.
CONTROL AND BLANK SYSTEM
- Inoculum blank: three flasks
- Abiotic sterile control: three flasks
- Toxicity control: one flask
Reference substance
- Reference substance:
- other: Sodium benzoate
Results and discussion
- Preliminary study:
- None
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 73.1
- Sampling time:
- 28 d
- Remarks on result:
- other: range 65.4 to 80.5
- Details on results:
- - The test item reached the criterion for ready biodegradability for surfactants in detergents (60% of ThCO2 within 28 days), but did not fullfil the 10 day window (not applied to surfactants).
- The degradation extent in the toxicity control was above 25% in 14 days based on ThCO2 (67.3%).
BOD5 / COD results
- Results with reference substance:
- - Sodium benzoate added to the reference vessels at a corresponding concentration of 20mg/l organic carbon.
- The biodegradation of the reference compound reached the pass level of 60% ThCO2 by day 4, passing the validity criteria.
Any other information on results incl. tables
Table 1: Ultimate biodegradation after x days [% of ThCO2]
reactor |
day |
0 |
4 |
7 |
11 |
14 |
21 |
28 |
29 |
7 8 9 |
Test flasks |
0 |
10.7 |
26.4 |
40.8 |
49.2 |
59.7 |
71.4 |
73.4 |
0 |
13.0 |
24.4 |
38.4 |
45.3 |
55.5 |
65.2 |
65.4 |
||
0 |
7.5 |
26.3 |
41.9 |
52.3 |
70.9 |
80.8 |
80.5 |
||
mean |
0 |
10.4 |
25.7 |
40.3 |
48.9 |
62.0 |
72.5 |
73.1 |
|
4 5 6 |
reference flasks |
0 |
70.6 |
77.2 |
81.2 |
84.1 |
84.1 |
88.8 |
88.2 |
0 |
52.7 |
76.3 |
81.5 |
84.0 |
87.8 |
89.5 |
89.6 |
||
0 |
74.8 |
80.8 |
89.0 |
91.0 |
91.9 |
94.0 |
94.8 |
||
mean |
0 |
66.1 |
78.1 |
83.9 |
86.4 |
87.9 |
90.8 |
90.9 |
|
10 |
Toxicity control Test item Reference item |
0 |
40.0 |
50.0 |
61.5 |
67.3 |
73.5 |
80.8 |
83.4 |
Table 2: Mean CO2-evolution of blank flasks after x days
Day |
0 |
4 |
7 |
11 |
14 |
21 |
28 |
29 |
CO2-evolution [mg/l] |
0 |
9.4 |
12.5 |
15.4 |
16.7 |
19.4 |
22.6 |
23.9 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The ready biodegradability of the test substance Phosphoric acid, mixed lauryl and myristyl esters was determined to be 73.1% based on ThCO2 after 28 days, fulfilling the criterion for ready biodegradability for detergents.
- Executive summary:
The ready biodegradability of the test substance Phosphoric acid, mixed lauryl and myristyl esters was assessed in a study carried out according to the OECD 301B guidelines (modified sturm test) and in compliance with GLP. It should be noted that assessment of the carbon content of the test item was not conducted according to GLP, but was accredited with DIN EN ISO/IEC 17025:2005. Biodegradation was assessed using test substance at an initital ThCO2 concentration of 19.5 -20mg/l and inoculum from activated sludge from a domestic sewage works. Biodegradation was measured over a 28 day period and measured on average 73.1% biodegradation based on ThCO2 after 28 days (ranging from 65.4 to 80.5%). The 60% biodegradation threshold within the 10 day window was not achieved, however as this test substance is a surfactant, this is criteria does not apply. No signs of toxicity were found in the toxicity controls. Based on this the test substance is considered readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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