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EC number: 210-933-7 | CAS number: 626-17-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994; specific dates not available.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- GLP study conducted by Japanese laboratories at the request of Japanese government. Report is translated by approved translation company.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Benzene-1,3-dicarbonitrile
- EC Number:
- 210-933-7
- EC Name:
- Benzene-1,3-dicarbonitrile
- Cas Number:
- 626-17-5
- Molecular formula:
- C8H4N2
- IUPAC Name:
- benzene-1,3-dicarbonitrile
- Test material form:
- solid: flakes
- Details on test material:
- Test substance name: Isophthalonitrile
Chemical name: 1,3-dicyanobenzene
CAS number: 626-17-5
EC number: 210-933-7
LOT number: JB1906150101
Purity: 99.8 % (w/w %)
Appearance: Off white solid flakes
Manufacture date: 15 June 2019
Expiry date:15 June 2020
Storage condition: Room temperature (15-25oC. below 70 RH%), protected from humidity
Constituent 1
- Specific details on test material used for the study:
- Not specified
Method
- Target gene:
- Histidine and Tryptophan
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 prepared by enzyme induction from seven-week-old Sprague-Dawley male rats using combined administration of phenobarbital (PB) and 5,6-benzoflavone (BF) (Kikkoman, Lot No. RAA-317, prepared on October 27, 1994) was used. The doses of PB and BF were PB 30 mg/kg on Day 1, PB 60 mg/kg on Day 2, PB 60 mg/kg and BF 80 mg/kg on Day 3, and PB 60 mg/kg on Day 4. Because all were intraperitoneally administered, rat dissection and S9 preparation were on Day 5.
- Test concentrations with justification for top dose:
- 0, 50, 150, 500, 1500, 5000 μg per plate
- Vehicle / solvent:
- Acetone
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide, 2-Aminoanthracene
- Details on test system and experimental conditions:
- 0.1 mL of the test substance preparation solution, 0.5 mL of phosphate buffer (0.5 mL S9 mix in the S9 mix addition test), 0.1 mL of the test bacteria solution, and 2 mL of top agar were mixed in a small test tube, and then poured into a synthetic medium plate and hardened. As control groups, several commonly used solvents and positive control substance solutions were used instead of the test substance preparation.
Culturing was conducted at 37°C for 48 hours, and the number of mutant colonies produced was calculated. The presence or absence of antibacterial activity was determined from the state of the fungal film on the surface of the agar observed with the naked eye or under a stereomicroscope. In the dose setting study, three plates each were used for the solvent and positive control groups and one for each dose. In this test, three samples were used for both control groups and each dose, and the average value and the standard deviation were obtained. The dose setting test was performed once, and the main test was performed twice on the same dose, and the reproducibility of the results was confirmed. - Rationale for test conditions:
- As per guideline.
- Evaluation criteria:
- The test substance was determined to be mutagenic (positive) in this test system when, among the five types of test bacteria used, the average number of mutant colonies on the plate containing the test substance was higher than that of the solvent control in one or more test bacteria whether the S9 mix was added or the S9 mix was not added and when the increase was reproducible or dose-dependent.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- The DCB doses were tested with a common ratio of 2 in a range from 313 to 5000 μg per plate in both the S9 mix addition test and the S9 mix-free test. As a result, in these two tests, no increase in the number of mutant colonies more than twice that of the solvent control value was observed for the five types of test bacteria used in the S9 mix addition test and the S9 mix-free test.
In all of the DCB tests conducted, the positive control group showed an increase in the number of mutant colonies for all of the test bacteria, but the number of mutant colonies measured in the solvent control group was within the range of historical control values. This confirmed the effectiveness of the test system.
Dose Setting Test
When DCB was tested at a common ratio of about 3 in a range from 50 to 5000 μm per plate, no antibacterial activity was observed in any of the test bacteria in the S9 mix addition test and the S9-free test.
Therefore, the maximum dose in this study was 5000 μg per plate for both the S9 mix addition test and the S9 mix-free test.
Main Tests
The results from the two main tests are shown in Tables 2 and 3, respectively. The DCB doses were tested with a common ratio of 2 in a range from 313 to 5000 μg per plate in both the S9 mix addition test and the S9 mix-free test. As a result, in these two tests, no increase in the number of mutant colonies more than twice that of the solvent control value was observed for the five types of test bacteria used in the S9 mix addition test and the S9 mix-free test.
In all of the DCB tests conducted, the positive control group showed an increase in the number of mutant colonies for all of the test bacteria, but the number of mutant colonies measured in the solvent control group was within the range of historical control values. This confirmed the effectiveness of the test system.
Based on these results, 1,3-dicyanobenzene was determined to be non-mutagenic (negative) in the test system used. - Remarks on result:
- other:
- Remarks:
- No mutagenic
Applicant's summary and conclusion
- Conclusions:
- Based on these results, 1,3-dicyanobenzene was determined to be non-mutagenic (negative) in the test system used.
- Executive summary:
The mutagenicity in 1,3-dicyanobenzene was examined in a reverse mutation test using bacteria and a negative result was obtained.
With five strains of Salmonella typhimurium (TA100, TA1535, TA98, TA1537) and Escherichia coli WP2 uvrA serving as the test bacteria, a dose setting test and a main test were conducted using the plate method under the conditions in which an S9 mix was added and not added.When a dose setting test was performed at doses from 50 to 5000 μg per plate, antibacterial activity was not observed in any of the test bacteria in the S9 mix free test and in the S9 mix test. Therefore, the S9 mix free test and S9 mix test were conducted at doses set in a range from 313 to 5000 μg/plate.
As a result, no increase in the number of revertant colonies more than twice that of the solvent control value was observed at any dose in the five types of test bacteria used in the two tests. 1,3-dicyanobenzene was determined not to be mutagenic (negative) in the test systems used.
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