[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 January 2018 to 16 March 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Reliable experimental study following OECD 202 guidelines (2004) and EC No. 440/2008 Method C.2 adopted 2008 and GLP standards.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test item is listed as ATHC (CAS # 24817-92-3) and is pale yellow slightly viscous liquid obtained from the Sponsor, Vertellus, with 99.4% purity and stored at room temperature in the dark.

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

The test was carried out using first instar Daphnia magna derived from in-house laboratory cultures.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20°C to 21 °C

pH:

7.7 to 8.1

Dissolved oxygen:

8.6 to 9.3 mg O2/L

Nominal and measured concentrations:

A preliminary media preparation trial indicated that a saturated solution method of preparation was appropriate for this test item. Based on the results of the range-finding test a "limit test" was conducted at a concentration of 100% v/v saturated solution which corresponds to an initial loading rate 50 mg/L.

Reference substance (positive control):

yes

Remarks:

potassium dichromate as the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 other: % v/v saturated solution.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

100% v/v saturated solution prepared from a test solution with a loading rate of 50 mg/L.

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

Test solution prepared with a loading rate of 50 mg/L resulting in a 100% v/v saturation solution.

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 other: % v/v saturated solution.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

100% v/v saturated solution prepared from a test solution with a loading rate of 50 mg/L.

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

Test solution prepared with a loading rate of 50 mg/L resulting in a 100% v/v saturation solution.

Results with reference substance (positive control):

The results from the positive control with potassium dichromate were within the normal ranges for this reference item.

Validity criteria fulfilled:

yes

Remarks:

The test was considered valid since none of the control daphnids showed immobilisation or other signs of disease or stress and the oxygen concentration at the end of the test was equal to or greater than 3 mg/L in the control and test vessels.

Conclusions:

The test substance did not induce acute immobilisation of Daphnia magna at the nominal concentration limit test after 48 h of exposure; therefore, there were no toxic effects at saturation. The effects concentrations were based on the nominal test concentration of 100% v/v saturated solution which was equivalent to an initial nominal loading rate of 50 mg/L. The effects concentrations were reported both as 48-h EC50 > 100% v/v saturated solution and 48-h EL50 >50 mg/L. The no effects concentrations were reported both as 48-h NOEC of 100% v/v saturated solution and 48-h NOELR of 50 mg/L.

Description of key information

The key study was a static acute invertebrate toxicity test on the effects of the substance on the freshwater invertebrate Daphnia magna following OECD Guideline 202. The test substance did not induce acute immobilisation of Daphnia magna at the nominal concentration limit test after 48 h of exposure; therefore, there were no toxic effects at saturation. The effects concentrations were based on the nominal test concentration of 100% v/v saturated solution which was equivalent to an initial nominal loading rate of 50 mg/L. The effects concentrations were reported both as 48-h EC50 > 100% v/v saturated solution and 48-h EL50 >50 mg/L. The no effects concentrations were reported both as 48-h NOEC of 100% v/v saturated solution and 48-h NOELR of 50 mg/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

50 mg/L