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EC number: 616-651-4 | CAS number: 787582-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-01-28 to 2008-02-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1988-05-30
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984-04-04
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test item is not soluble in water. Therefore, the test item was directly dosed into each test flask and tap water was added. For complete suspension of the test item, this mixture was stirred for about 24 hours at 21 °C in the dark / diffuse light before adding the inoculum. The test flasks were tightly closed to avoid water loss by evaporation.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Species / Origin:
Activated Sludge, micro organisms from a domestic waste water treatment plant was supplied by the municipal sewage treatment plant Darmstadt, Germany.
Conditioning:
The activated sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and again centrifuged. The latter procedure was repeated twice. The washed sludge was resuspended in tap water and sewage feed (50 mL per L) was added. The sludge was kept at room temperature under continuous aeration until use over night. Immediately before use, the dry weight of the activated sludge was determined and diluted to 3.8 mg/L with tap water. The pH of the activated sludge was 7.8 and therefore no adjustment necessary. - Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21°C during dissolution phase (24 hours); 19-21 °C during pre-incubation (3 hours); 18-20°C during evaluation period
- pH:
- At the start of the study the pH of the activated sludge was 7.8 and therefore no adjustment was necessary.
During the study pH values of 7.4 at the start and 8.6 at the end of study (after 3h) were determined. - Dissolved oxygen:
- The concentration of dissolved oxygen did not drop below 2.5 mg O2/L during the incubation period and just before the measurements of the respiration rates the oxygen concentrations were at least 8.5 mg O2/L and thus >6.5 mg O2/L.
- Nominal and measured concentrations:
- Nominal concentrations: 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks of approximately 1 litre volume and Karlsruher flasks of 250 mL volume
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 3.8 g/L
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: deionised water
Synthetic sewage:
16 g peptone
11 g meat extract
3 g urea
0.7g NaCl
0.4 g CaCl2 * 2H20
0.2 g MgSO4 * 7H2O
2.8 g K2HP04
filled up to 1 liter with deionised water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no detials available
- Light intensity: no detials available
EFFECT PARAMETERS MEASURED
respiration rate (Karlsruher flask)
The pH and the oxygen concentrations were determined at the start and at the end of the incubation period in all treatments. The water temperature was measured in one control medium at the start and the end of the incubation period.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: factor 3.2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Based on measured inhibition rates, the 3 -hour EC10, EC20 and EC50 could not be quantified because up to the highest nominal test concentration of 1000 mg/L less than 10% inhibition was noted after three hours incubation. No dose related response was found. The test according to OECD 209 guideline does not provide a threshold value for the determination of a NOEC. If a deviation of less ≤ 15% between test item treated activated sludge and control is considered not to be an effect, the NOEC of the test item will be established above 1000 mg/L.
Validity Criteria of the Study
Inoculum Control: The respiration rates of the two controls did not differ by more than 15 %.
Reference Item: The 3-hour EC50 of the reference item 3,5-Dichlorophenol was determined to be 10.4 mg/L and is thus in the range of 5 to 30 mg/L for the used activated sludge batch.
Dissolved Oxygen: The concentration of dissolved oxygen did not drop below 2.5 mg O2/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 8.5 mg O2/L and thus >6.5 mg O2/L. - Results with reference substance (positive control):
- The 3 -hour EC50 of 3,5 -Dichlorophenol was calculated to be 10.4 mg/L with 95% confidence limits of 7.4 - 14.6 mg/L.
- Reported statistics and error estimates:
- The 3-hour EC50 including the 95 %-confidence limits of the reference item was calculated by Probit analysis. The 3-hour EC20 and EC50 of the test item could not be calculated due to the low toxicity of the test item to micro organisms under the tested conditions.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test according to OECD 209 guideline does not provide a threshold value for the determination of a EC50. Up to the highest nominal concentration of 1000 mg/L no respiration inhibition was detected (EC50 > 1000 mg/L, NOEC = 1000 mg/L).
- Executive summary:
The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions according to OECD 209 and EU Method C.11. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours. The test concentrations were 10, 32,100, 320 and 1000 mg/L test item and 3.2,10 and 32 mg/L 3,5-Dichlorophenol. Additionally two inoculum controls were evaluated. The test item is not soluble in water. Therefore, the test item was directly weighted into the test vessels and stirred for about 24 hours before using in the experiment. The respiration rates of the activated sludge treated with the test item at concentrations between 10 mg/L and 1000 mg/L differed by less than 10% from control. Based on measured inhibition rates, the 3-hour EC10, EC20 and EC50 could not be quantified because up to the highest nominal test concentration of 1000 mg/L less than 10 % inhibition was noted after three hours incubation. No dose related response was found. The test according to OECD 209 guideline does not provide a threshold value for the determination of a NOEC. If a deviation of less < 15% between test item treated activated sludge and control is considered not be an effect, the NOEC of the test item will be established to be1000 mg/L. The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be10.4 mg/L with 95 % confidence limits of 7.4 -14.6 mg/L. Up to the highest nominal concentration of 1000 mg/L no respiration inhibition was detected (EC50 > 1000 mg/L, NOEC = 1000 mg/L).
Reference
Description of key information
The test according to OECD 209 guideline does not provide a threshold value for the determination of a EC50. Up to the highest nominal concentration of 1000 mg/L no respiration inhibition was detected (EC50 > 1000 mg/L, NOEC = 1000 mg/L) (reference 6.1.7-1).
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions according to OECD 209 and EU Method C.11. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours. The test concentrations were 10, 32,100, 320 and 1000 mg/L test item and 3.2,10 and 32 mg/L 3,5-Dichlorophenol. Additionally two inoculum controls were evaluated. The test item is not soluble in water. Therefore, the test item was directly weighted into the test vessels and stirred for about 24 hours before using in the experiment. The respiration rates of the activated sludge treated with the test item at concentrations between 10 mg/L and 1000 mg/L differed by less than 10% from control. Based on measured inhibition rates, the 3-hour EC10, EC20 and EC50 could not be quantified because up to the highest nominal test concentration of 1000 mg/L less than 10 % inhibition was noted after three hours incubation. No dose related response was found. The test according to OECD 209 guideline does not provide a threshold value for the determination of a NOEC. If a deviation of less < 15% between test item treated activated sludge and control is considered not be an effect, the NOEC of the test item will be established to be1000 mg/L. The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be10.4 mg/L with 95 % confidence limits of 7.4 -14.6 mg/L. Up to the highest nominal concentration of 1000 mg/L no respiration inhibition was detected (EC50 > 1000 mg/L, NOEC = 1000 mg/L) (reference 5.1.7 -1).
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