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EC number: 817-766-4 | CAS number: 91891-42-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 Oct - 15 Dec 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene
- EC Number:
- 817-766-4
- Cas Number:
- 91891-42-8
- Molecular formula:
- C6F12O
- IUPAC Name:
- 1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: MA-3-HO-ANL 1600356
- Expiration date of the lot/batch: 31 Aug 2020
- Purity test date: 05 Feb 2018
- Purity: 96.9%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature container flushed with nitrogen
- Stability under test conditions: Yes
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations sampled.
- Volume: 42.5 mL, one complete vial (see sampling method).
- Sampling method: Three extra sample vials for each concentration were made, without inoculation to be sacrificed for analytic monitoring (one each for t=0h, t=24h, and t=72 h) and incubated under similar conditions to the inoculated vials.
- Sample storage conditions before analysis: Samples were analyzed on the day of sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Method: The test substance is volatile. Therefore, 42.5mL air-tight vials were completely filled with medium and closed with a septum-sealed screw cap. MA-3 (2.5 µL) was then injected through the septum with gas-tight syringe to achieve a loading rate of 100 mg/L. Thereafter the septum was sealed using parafilm. Vials were rotated slowly for four days, and then centrifuged at 500g for 90 minutes to pull dissolved test substance into a single drop at the bottom of the vial. A dilution series was also made of additional 100% saturated samples. Dilutions were made by injecting exact volumes of the saturated solution through the septa of vials that were complete filled with medium. Excess medium was allowed to drain through a vent needle during injection.
- Controls: Blank only
- Evidence of undissolved material: Test solutions were clear and colorless. Undiluted (100% saturated) samples contained a visible small droplet of undissolved test substance at the bottom of the vials.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Common name: Pseudokirchneriella subcapitata
- Strain: NIVA CHL 1
- Source (laboratory, culture collection): In-house laboratory culture
.
- Method of cultivation: Stock cultures were maintained in M1 medium (Nederlandse Praktijk Richtlijn no. 6505).
NaNO3, 500 mg/l
K2HPO4, 39.5 mg/l
MgSO4∙7H2O, 75 mg/l
Na2CO3, 20 mg/l
C6H8O7∙H2O, 6 mg/l
NH4NO3, 330 mg/l
CaCl2∙2H2O, 35 mg/l
C6H5FeO7∙xH2O, 6 mg/l
H3BO3, 2∙9 mg/l
MnCl2∙4H2O, 1.81 mg/l
ZnCl2, 0.11 mg/l
CuSO4∙5H2O, 0.08 mg/l
(NH4)6Mo7O24∙4H2O, 0.018 mg/l
ACCLIMATION
Cells for the final test were taken directly from stock culture. Algae were acclimated only for preliminary testing. Three days before the start of the test, cells from the algal stock culture were inoculated in culture medium (M2 according to OECD 201) at a cell density of 1e+04 cells/mL. The pre-culture was maintained under the same conditions as used in the test. The cell density was measured immediately before use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 21 - 23 °C
- pH:
- 7.5 - 7.8
- Dissolved oxygen:
- n/a
- Salinity:
- n/a
- Conductivity:
- n/a
- Nominal and measured concentrations:
- see Table 1
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass VOA vials, completely filled and closed with septa. Fill volume ca. 42.5 m/L
- Agitation: No
- Initial cells density: 1E+04 cells/mL
- Control end cells density: 114.3E+04 cells/mL
- No. of vessels per concentration (replicates): Three replicates per day, sacrificed for cell counts.
- No. of vessels per control (replicates): Six replicates per day, sacrificed for cell counts.
GROWTH MEDIUM
- Standard medium used: yes, cells grown in M1. Preacclimation in adjusted M2 before test.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard medium (adjusted M2) prepared in reverse osmosis purified water. M2 (per OECD 201) was adjusted by addition of 300 mg/L NaHCO3 and 6 mM HEPES buffer, final pH 7.1 ± 0.3
- Ca/mg ratio: 1
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: Continuous
- Light intensity and quality: Fluorescent (TL-D) lamps with a light intensity within the range of 84 to
85 μE/(m²∙s). Test vessels were placed randomly and randomly repositioned every day.
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Cells were counted using a microscope and a counting chamber to determine inoculum density. Thereafter, cell densities were determined by spectrophotometric measurement of samples at 680 nm using a spectrophotometer with cuvettes (pathlength = 10 mm).
- Appearance: at the end of the test, microscopic examination was done on the 10% dilution to observe for any abnormal appearance of the algae.
TEST CONCENTRATIONS
- Range finding study: yes, done after initial limit test
- Test concentrations: Control, undiluted WSF, and 1.0% and 10% dilutions of WSF
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 270 µg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI, 154 - 620 µg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 6.9 µg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI, 2.1 - 14 µg/L
- Details on results:
- - Exponential growth in the control: yes (Table 3, 4, Fig. 1)
- Observation of abnormalities: None observed. Microscopic observations at the end of the test revealed a normal and healthy appearance of the algal cells exposed to 10% saturated solution (the lowest concentration showing a significant effect on cell density) when compared to the control.
- Any stimulation of growth found in any treatment: no
- Effect concentrations exceeding solubility of substance in test medium: no
- Other: Test vials were sacrificed at each time point. Decline in average cell number between time points does not reflect a reduction in cell count within the same test chambers. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 1.05 mg/L. Historical range for the reference substance at the contract lab lies between 0.82 and 2.6 mg/L
- Other: Reference substance toxicity assay conducted between the rangefinder and the definitive study, appr 36 days before the definitive test. - Reported statistics and error estimates:
- Growth rate data were normally distributed by Shapiro-Wilk's test, p(W) = 0.047 > 0.01. Variance of growth rate data were not homogeneous by Levene's test, p(F) = 0.007 < 0.01. The NOEC could not determined due to excessive variability between exposure levels relative to biological effect. The EC10 was determined instead. EC10 and EC50 were determined based on probit analysis using linear maximum likelihood regression with the percentages of growth rate inhibition v. logarithms of the corresponding time-weighted average concentrations. Analysis was done using ToxRat Professional v3.2.1 (ToxRat Solutions GmbH, Germany)
Any other information on results incl. tables
Table 3, cell densities
Time (hr) |
Replicate |
Control |
0.20 µg/L |
5.4 µg/L |
6.8 µg/L |
21 µg/L |
301 µg/L |
0 |
1 |
1.000 |
1.000 |
1.000 |
1.000 |
1.000 |
1.000 |
2 |
1.000 |
1.000 |
1.000 |
1.000 |
1.000 |
1.000 |
|
3 |
1.000 |
1.000 |
1.000 |
1.000 |
1.000 |
1.000 |
|
4 |
1.000 |
||||||
5 |
1.000 |
||||||
6 |
1.000 |
||||||
Mean |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
|
24 |
1 |
6.709 |
5.221 |
5.221 |
4.649 |
3.262 |
2.267 |
2 |
4.032 |
4.490 |
8.490 |
4.439 |
4.350 |
2.569 |
|
3 |
4.865 |
3.987 |
3.631 |
4.725 |
6.309 |
1.348 |
|
4 |
4.146 |
||||||
5 |
4.356 |
||||||
6 |
2.359 |
||||||
Mean |
4.4 |
4.6 |
5.8 |
4.6 |
4.6 |
2.2 |
|
48 |
1 |
18.188 |
32.523 |
34.348 |
20.109 |
23.537 |
4.821 |
2 |
18.322 |
34.634 |
20.459 |
23.581 |
11.282 |
5.081 |
|
3 |
24.510 |
28.497 |
29.935 |
21.852 |
11.384 |
6.983 |
|
4 |
11.759 |
||||||
5 |
28.656 |
||||||
6 |
23.581 |
||||||
Mean |
20.8 |
31.9 |
28.2 |
21.8 |
15.4 |
5.6 |
|
72 |
1 |
111.464 |
131.173 |
119.681 |
103.738 |
18.029 |
18.532 |
2 |
111.388 |
130.511 |
102.955 |
57.306 |
26.278 |
11.428 |
|
3 |
98.904 |
134.423 |
100.539 |
87.298 |
65.669 |
6.054 |
|
4 |
116.552 |
||||||
5 |
128.635 |
||||||
6 |
118.816 |
||||||
Mean |
114.3 |
132.0 |
107.7 |
82.8¹ |
36.7¹ |
12.0¹ |
1, statistically significant effect on yield
Table 4, section and average growth rates
Section |
Replicate |
Control |
0.20 µg/L |
5.4 µg/L |
6.8 µg/L |
21 µg/L |
301 µg/L |
0-24 hr |
1 |
1.903 |
1.653 |
1.653 |
1.537 |
1.182 |
0.966 |
2 |
1.394 |
1.502 |
2.139 |
1.49 |
1.47 |
0.944 |
|
3 |
1.582 |
1.383 |
1.29 |
1.553 |
1.842 |
0.299 |
|
4 |
1.422 |
||||||
5 |
1.472 |
||||||
6 |
0.858 |
||||||
Mean |
1.439 |
1.513 |
1.694 |
1.527 |
1.498 |
0.736 |
|
24-48 hr |
1 |
0.997 |
1.829 |
1.884 |
1.465 |
1.976 |
0.607 |
2 |
1.514 |
2.043 |
0.88 |
1.67 |
0.953 |
0.682 |
|
3 |
1.617 |
1.967 |
2.11 |
1.531 |
0.59 |
1.645 |
|
4 |
1.042 |
||||||
5 |
1.884 |
||||||
6 |
2.302 |
||||||
Mean |
1.559 |
1.946 |
1.624 |
1.555 |
1.173 |
0.978 |
|
48-72 h |
1 |
1.813 |
1.395 |
1.248 |
1.641 |
-0.267 |
1.347 |
2 |
1.805 |
1.327 |
1.616 |
0.888 |
0.846 |
0.811 |
|
3 |
1.395 |
1.551 |
1.212 |
1.385 |
1.752 |
-0.143 |
|
4 |
2.294 |
||||||
5 |
1.502 |
||||||
6 |
1.617 |
||||||
Mean |
1.738¹ |
1.424 |
1.359 |
1.305 |
0.777 |
0.671 |
|
0-72 hr |
1 |
1.571 |
1.626 |
1.595 |
1.547 |
0.964 |
0.973 |
2 |
1.571 |
1.624 |
1.545 |
1.349 |
1.09 |
0.812 |
|
3 |
1.531 |
1.634 |
1.537 |
1.49 |
1.395 |
0.6 |
|
4 |
1.586 |
||||||
5 |
1.619 |
||||||
6 |
1.593 |
||||||
Mean |
1.579² |
1.628 |
1.559 |
1.462 |
1.149 |
0.795 |
|
Percent |
Inhibition |
─ |
-(3.1) |
1.2 |
7.4 |
27 |
50³ |
1, CV of control section rates, 26%
2, CV of control average growth rates, 1.8%
3, effect statistically significant
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- exponential increase >16/test period (114), <7% CV for average growth rate (1.8%), <35% CV for section specific growth rate (26%)
- Conclusions:
- MA-3 72-h EC50, 270 µg/L; MA-3 72-h EC10, 6.9 µgL (OECD 201)
- Executive summary:
Toxicity of MA-3 to the freshwater alga Pseudokirchneriella subcapitata was assessed according to OECD201. The test substance is highly volatile. All tests were conducted in glass VOA vials, completely filled with medium and closed with a septum cap. All transfers were made through the septum with gas-tight syringe. A water soluble fraction was made at a loading rate of 100 mg/L, with residual undissolved test material allowed to remain in the test vials. Dilutions of the 100 mg/L stock had no residual undissolved material. Exposure concentrations were analytically determined in parallel test vials. Analytically determined concentrations declined throughout the test except in the undiluted test solution containing undissolved material. Time-weighted average concentrations were used to calculate effect concentration. The 72-hour EC50 (growth rate) was 270 µg/L. The 72-hour EC10 was 6.9 µg/L.
The study was conducted according to internationally accepted test guidelines and in accord with GLP criteria, with test substance concentrations were confirmed analytically. Test substance concentrations were not stable during the test. The highest test concentration, at which the bulk of effects were observed, was a different treatment than the dilutions (it is possible that the EC50 would have been higher or not attained if all samples were treated identically). The study is deemed reliable with restrictions. It is suitable for Risk Assessment, Classification & Labeling, and PBT Analysis.
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