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EC number: 208-174-1 | CAS number: 513-86-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- Acetoin
- EC Number:
- 208-174-1
- EC Name:
- Acetoin
- Cas Number:
- 513-86-0
- Molecular formula:
- C4H8O2
- IUPAC Name:
- 3-hydroxybutan-2-one
- Test material form:
- liquid
- Remarks:
- a colourless liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Doses:
- The starting dose could be selected from the fixed dose levels of 5, 50, 300, and 2000 mg/kg body weight. Available information indicated that the test item is likely to be non-toxic with regard to acute toxicity. A limit dose of 2000 mg/kg body weight was therefore used as a starting dose. One group of 3 females was dosed. Test item-related mortality was not observed during 24 hours and therefore in a second step another 3 females were treated at the same dose level.
- No. of animals per sex per dose:
- The starting dose could be selected from the fixed dose levels of 5, 50, 300, and 2000 mg/kg body weight. Available information indicated that the test item is likely to be non-toxic with regard to acute toxicity. A limit dose of 2000 mg/kg body weight was therefore used as a starting dose. One group of 3 females was dosed. Test item-related mortality was not observed during 24 hours and therefore in a second step another 3 females were treated at the same dose level.
Results and discussion
- Preliminary study:
- The test item Acetoin was administered to 6 females Wistar rats at a limit dose of 2000 mg/kg. No mortality occurred during the study. No clinical signs related to the administration of the test item were observed during the first 4 hours or after the 14-day observation period. Stagnation of body weight in three animals between the first and second week was observed. The macroscopical examination of the animals at the end of the study did not reveal treatment-related changes.
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- 0
Any other information on results incl. tables
All (6/6 females) animals survived the limit dose of 2000 mg/kg body weight.
Table1Administration Results
Sex |
Dose |
ID |
Result |
Sex |
Dose |
ID |
Result |
♀ |
2000 mg/kg |
1 |
alive |
♀ |
2000 mg/kg |
4 |
alive |
2 |
alive |
5 |
alive |
||||
3 |
alive |
6 |
alive |
Table3Body Weight
Sex |
Dose |
ID |
Body Weight (g) |
Body Weight Difference (g) |
||||
Initial |
Week 1 |
Week 2 |
Week 1-Initial |
Week 2-Initial |
Week 2-Week 1 |
|||
♀ |
2000 mg/kg |
1 |
170 |
205 |
205 |
35 |
35 |
0 |
2 |
185 |
205 |
220 |
20 |
35 |
15 |
||
3 |
170 |
195 |
200 |
25 |
30 |
5 |
||
4 |
180 |
205 |
210 |
25 |
30 |
5 |
||
5 |
190 |
220 |
220 |
30 |
30 |
0 |
||
6 |
190 |
210 |
210 |
20 |
20 |
0 |
All animals were necropsied. During necropsy, no macroscopic findings were observed.
Table4NecropsyResults
Sex |
Dose |
ID |
Result |
Sex |
Dose |
ID |
Result |
♀ |
2000 mg/kg |
1 |
no findings |
♀ |
2000 mg/kg |
4 |
no findings |
2 |
no findings |
5 |
no findings |
||||
3 |
no findings |
6 |
no findings |
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- The LD50 of the test item Acetoin is greater than 2000 mg/kg body weight after single oral administration to Wistar rats.
- Executive summary:
Based onAnnex 2d Test Procedure with a Starting Dose of 2000 mg/kg body weight of OECD Guideline 423itcan be concluded that thetest item Acetoinis classified in GHS Category 5/Unclassified with a LD50cutoff value equal to or greater than5000 mg/kg body weight,after single oral administration to Wistar rats.
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