4-Methyl-N-[3-(triethoxysilyl)propyl]-2-pentanimine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

07 February 2018 - 03 May 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13 April 2004

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

After 0h; 24h and 48h exposure, all replicates of control and treatment groups were sampled (5 ml), one sample per replicate, 4 samples per treatment group and control
Of each sampled treatment, one of the analytical samples from 0h, 24h and 48h was sent to the analytical laboratory at the test site for chemical analysis. The remaining samples were stored as retain samples in the freezer (<=18°C) until finalization of the study.

Vehicle:

no

Details on test solutions:

The stock solution was prepared as Water Soluble Fraction (WSF) by adding 400 mg test item to 1000 ml test medium and shaking for 24 h using an overhead shaker at 20.3 - 21.5 °C. The stock solution was allowed to settle for 1.5 h before decanting the upper part of the liquid column for use in the test. This decanted stock solution was used as highest tst item loading rate in the test. The negative control (test medium) was treated in the same way as the test item solution.
4 replicates, with 5 daphnids each, were prepared for each test item treatment and the negative control. The further test item loading rates were prepared by diluting the stock solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flee
- Source: federal environmental Agency, Berlin
- test organisms used were 0.17 - 21.75 h old at the start of the test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

the test was performed with Elendt M4 medium according to OECD 202 (2004)

Test temperature:

20.1-21.1

pH:

control: 7.5-7.9
test item treatment: 7.6-9.9

Dissolved oxygen:

conrol: 8.2-8.3
test item treatment: 8.0-8.2

Nominal and measured concentrations:

nominal: 0; 25; 50; 100; 200; 400 mg/l
measured (geometrical mean): 0; 22.68; 43.85; 84.83; 170.63; 305.25 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass beakers
- Type (delete if not applicable): open
- fill volume: 40 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light, 8h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : the swimming capability of the daphnids was determined after 24h and 48h exposure. pH and oxygen concentration was measured after 0h; 24h, and 48h, the test vessels were kept in an icubator with constant temperature

RANGE-FINDING STUDY
- Test concentrations: 50; 100; 200 mg/l
- Results used to determine the conditions for the definitive study: no immobility in each concentration was observed after 48h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

265.7 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

170 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

84.8 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Nominal test item loading rate (mg/l)	Daphnia introduced				Immobile Daphnia (replicates 1-4)								Immobility(%)
	0 h				24 h				48 h
	1	2	3	4	1	2	3	4	1	2	3	4	24 h	48 h
control	5	5	5	5	0	0	0	0	0	0	0	0	0	0
25	5	5	5	5	0	0	0	0	0	0	0	0	0	0
50	5	5	5	5	0	0	0	0	0	0	0	0	0	0
100	5	5	5	5	0	0	0	0	0	0	0	0	0	0
200	5	5	5	5	2	0	0	1	3	0	0	1	15	20
400	5	5	5	5	1	0	1	2	2	2	4	4	20	20

Validity criteria fulfilled:

yes

Conclusions:

EC50 = 265.7 mg/l

Executive summary:

In a valid Daphnia magna acute immobilisation test according to OECD 202 under GLP conditions, the calculated EC50 = 265.7 mg/l based on analytically measured test item concentration (geometric mean concentration).

Description of key information

In a valid Daphnia magna acute immobilisation test according to OECD 202 under GLP conditions, the calculated EC50 = 265.7 mg/l based on analytically measured test item concentration (geometric mean concentration).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

265.7 mg/L