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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 January 2018 - 11 July 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
Version / remarks:
September 2014
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
source: municipal wastewater treatment plant Breisgauer Bucht
concentration: 4 mg dry solids (d.s.) per litre
sampling data: 17 January 2018
dry solid content of activated sludge: 3.8 g/l (triplicate measurements)
the activated sludge was washed twice with tape water by settling the sludge, decanting the supernatant an re-suspending the sludge. In order to keep the tIC of the blank at a low level, the activated sludge was pre-
Duration of test (contact time):
28 d
Initial conc.:
2.9 µg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Value:
86.4
St. dev.:
11
Sampling time:
7 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
93.4
St. dev.:
12.7
Sampling time:
14 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
89.6
St. dev.:
9.3
Sampling time:
21 d
Key result
Parameter:
% degradation (CO2 evolution)
Value:
83.4
St. dev.:
12.1
Sampling time:
28 d
Details on results:
Toxicity control: The degradation extend in the toxicity control was 79% within 14 days. The test substance had no inhibitory effect on the inoculum.
Reference item: The reference substance sodium benzoate reached the pass level for ready biodegradability within 7 days (80.6%)
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Under the test conditions the substance was ready biodegradable
Executive summary:

In an valid OECD 310, CO2 Headspace, Test under GLP the test substance was ready biodegradable.

Description of key information

In an valid OECD 310, CO2 Headspace Test under GLP the test substance is ready biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information